Specificity of CD8+ T cells from subunit-vaccinated and infected H-2b mice recognizing the 38 kDa antigen of Mycobacterium tuberculosis

Zhu, X.; Stauss, HJ; Iványi, J; Vordermeier, H. M.

doi:10.1093/intimm/9.11.1669

Cited by 31 publications

(16 citation statements)

References 45 publications

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“…Apart from the ability to make cytokines, CD8 ϩ T cells may also function as CTLs; the development of CD8 ϩ CTLs during acute murine tuberculosis has been documented (17,24,33,35,36,39). We demonstrated that, 3 weeks postchallenge, the lungs of memory mice contained CD8…”

Section: Cytotoxic Function Of Memory Cd8 ؉ T Cells Previously We Dmentioning

confidence: 75%

“…5). Although several antigens have been shown to be recognized by mycobacterium-specific CD8 ϩ T cells (14,28,35,39), the antigenic repertoire recognized by CD8 ϩ T cells in tuberculosis remains largely uncharacterized. Therefore, we have used infected macrophages as targets for specific CD8 ϩ CTLs to ensure that complete spectrum of antigens is presented to T cells during the assay.…”

Section: Cytotoxic Function Of Memory Cd8 ؉ T Cells Previously We Dmentioning

confidence: 99%

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CD8⁺T Cells Participate in the Memory Immune Response toMycobacterium tuberculosis

2001

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The contribution of CD8؉ T cells to the control of tuberculosis has been studied primarily during acute infection in mouse models. Memory or recall responses in tuberculosis are less well characterized, particularly with respect to the CD8 T-cell subset. In fact, there are published reports that CD8 ؉ T cells do not participate in the memory immune response to Mycobacterium tuberculosis. We examined the CD8 ؉ T-cell memory and local recall response to M. tuberculosis. To establish a memory immunity model, C57BL/6 mice were infected with M. tuberculosis, followed by treatment with anti-mycobacterial drugs and prolonged rest. ؉ and CD8 ؉ T cells in the lungs of immune mice expressed the activation marker CD69 and could be restimulated to produce gamma interferon (IFN-␥). In contrast, <6% of T cells in the lungs of naive challenged mice were CD69؉ at 1 week postchallenge, and IFN-␥ production was not observed at this time point. CD8؉ T cells from the lungs of both naive and memory mice after challenge were cytotoxic toward M. tuberculosis-infected macrophages. Our data indicate that memory and recall immunity to M. tuberculosis is comprised of both CD4؉ and CD8 ؉ T lymphocytes and that there is a rapid response of both subsets in the lungs following challenge. Control of acute tuberculosis in mice involves participation of CD8ϩ T cells (6,22,37), although a recent publication argues for a more important role for this subset in control of persistent infection (38). Recent studies on the development and activation of mycobacterium-specific CD8 ϩ T cells have indicated that substantial numbers of activated CD8 ϩ T lymphocytes with both cytokine-secreting and cytotoxic functions are present in the lungs during the acute phase of infection in mice (13,19,(33)(34)(35). However, whether this acute response culminates in the development of memory CD8 ϩ T-cell populations that can respond robustly to a secondary Mycobacterium tuberculosis challenge is not clear. It is believed that a proper memory response develops after an infection is cleared. CD8 ϩ T cells play a prominent role during memory immune responses in intracellular bacterial infections with Listeria monocytogenes (11) and Chlamydia pneumoniae (31). In the mouse model and in humans, M. tuberculosis can be a persistent or latent infection. To study the memory response in this infection, it is useful to first reduce or eliminate the bacterial burden in the mouse tissues. It has been reported that the treatment of infected mice with antibiotics leads to the development of an immune response capable of reducing the bacterial burden after challenge (4). The major role in combating bacterial challenge during memory immune responses so far has been attributed exclusively to CD4 ϩ T cells (4). Earlier studies have argued against the participation of memory CD8 ϩ T cells in the recall immune responses in a murine model of tuberculosis (3,4,30). However, the development of a memory CD8 ϩ T-cell population during M. tuberculosis infection is supported by the findings that ...

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Section: Cytotoxic Function Of Memory Cd8 ؉ T Cells Previously We Dmentioning

confidence: 75%

Section: Cytotoxic Function Of Memory Cd8 ؉ T Cells Previously We Dmentioning

confidence: 99%

CD8⁺T Cells Participate in the Memory Immune Response toMycobacterium tuberculosis

2001

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“…ϩ T cells specific for epitopes of the 38-kDa glycolipoprotein were isolated (20,36). However, with the exception of hsp65-specific CTLs generated by immunization with a cell line expressing this Ag (19), no other reports document the ability of CD8 ϩ CTLs to lyse M infected with live M. tuberculosis, which is an important component of protection by CTLs.…”

Section: Discussionmentioning

confidence: 99%

CD8+ CTL from Lungs ofMycobacterium tuberculosis-Infected Mice Express Perforin In Vivo and Lyse Infected Macrophages

Serbina¹,

Liu

Scanga³

et al. 2000

The Journal of Immunology

146

112

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CD8+ T lymphocytes have been implicated in the protective immune response against human and murine tuberculosis. However, the functional role that this cell subset plays during the resolution of infection remains controversial. In this study, we demonstrate the presence of Mycobacterium tuberculosis-specific CD8+ CTL in the lungs and lung-draining lymph nodes of mice infected with M. tuberculosis via the aerosol or i.v. route. These cells expressed perforin in vivo and specifically recognized and lysed M. tuberculosis-infected macrophages in a perforin-dependent manner after a short period of in vitro restimulation. The efficiency of lysis of infected macrophages was dependent upon the time allowed for interaction between macrophage and M. tuberculosis bacilli. Recognition of infected targets by CD8+ CTL was β2-microglobulin and MHC class I dependent and was not CD1d restricted. The presented data indicate that CD8+ T cells contribute to the protective immune response during M. tuberculosis infection by exerting cytotoxic function and lysing infected macrophages.

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“…Second, DNA vaccines may contribute to the improved protection against M. tuberculosis infection by priming both CD4 ϩ and CD8 ϩ T cells. DNA immunization can stimulate both T-cell subsets (19) and is more potent than mycobacteria at priming naive CD8 ϩ T cells (3,20). Third, BCG immunization may effectively amplify mycobacterium-specific CD8 ϩ T-cell responses primed by DNA immunization, as the requirements for activation of effector/ memory T cells are less stringent than those for their naive counterparts (18).…”

Section: Vol 69 2001mentioning

confidence: 99%

Priming by DNA Immunization Augments Protective Efficacy ofMycobacterium bovisBacille Calmette-Guerin against Tuberculosis

et al. 2001

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Specificity of CD8+ T cells from subunit-vaccinated and infected H-2b mice recognizing the 38 kDa antigen of Mycobacterium tuberculosis

Cited by 31 publications

References 45 publications

CD8⁺T Cells Participate in the Memory Immune Response toMycobacterium tuberculosis

CD8⁺T Cells Participate in the Memory Immune Response toMycobacterium tuberculosis

CD8+ CTL from Lungs ofMycobacterium tuberculosis-Infected Mice Express Perforin In Vivo and Lyse Infected Macrophages

Priming by DNA Immunization Augments Protective Efficacy ofMycobacterium bovisBacille Calmette-Guerin against Tuberculosis

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Specificity of CD8+ T cells from subunit-vaccinated and infected H-2b mice recognizing the 38 kDa antigen of Mycobacterium tuberculosis

Cited by 31 publications

References 45 publications

CD8+T Cells Participate in the Memory Immune Response toMycobacterium tuberculosis

CD8+T Cells Participate in the Memory Immune Response toMycobacterium tuberculosis

CD8+ CTL from Lungs ofMycobacterium tuberculosis-Infected Mice Express Perforin In Vivo and Lyse Infected Macrophages

Priming by DNA Immunization Augments Protective Efficacy ofMycobacterium bovisBacille Calmette-Guerin against Tuberculosis

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CD8⁺T Cells Participate in the Memory Immune Response toMycobacterium tuberculosis

CD8⁺T Cells Participate in the Memory Immune Response toMycobacterium tuberculosis