Speckle contrast optical spectroscopy, a non-invasive, diffuse optical method for measuring microvascular blood flow in tissue

Valdés, Claudia P.; Varma, Hari M.; Kristoffersen, Anna K.; Dragojević, Tanja; Culver, Joseph P.; Durdurán, Turgut

doi:10.1364/boe.5.002769

Cited by 139 publications

(131 citation statements)

References 28 publications

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“…Raw images are corrected for smear effects of EMCCD frame transfer [13, 21]. From the corrected images, the measured K s ( r ) are then calculated using (1) as modified for instrumentation noises (i.e., shot noise and dark noise) [16, 22]. Multiple detectors are defined in the selected ROI and each detector includes 3 × 3 pixel windows (each window contains 7 × 7 pixels with a pixel size of 8 μ m) [13].…”

Section: Methodsmentioning

confidence: 99%

Noncontact 3-D Speckle Contrast Diffuse Correlation Tomography of Tissue Blood Flow Distribution

Huang

Irwin

Zhao

et al. 2017

IEEE Trans. Med. Imaging

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Recent advancements in near-infrared diffuse correlation techniques and instrumentation have opened the path for versatile deep tissue microvasculature blood flow imaging systems. Despite this progress there remains a need for a completely noncontact, noninvasive device with high translatability from small/testing (animal) to large/target (human) subjects with trivial application on both. Accordingly, we discuss our newly developed setup which meets this demand, termed noncontact speckle contrast diffuse correlation tomography (nc_scDCT). The nc_scDCT provides fast, continuous, portable, noninvasive, and inexpensive acquisition of three-dimensional tomographic deep (up to 10 mm) tissue blood flow distributions with straightforward design and customization. The features presented include a finite-element-method implementation for incorporating complex tissue boundaries, fully noncontact hardware for avoiding tissue compression and interactions, rapid data collection with a diffuse speckle contrast method, reflectance-based design promoting experimental translation, extensibility to related techniques, and robust adjustable source and detector patterns and density for high resolution measurement with flexible regions of interest enabling unique application-specific setups. Validation is shown in the detection and characterization of both high and low contrasts in flow relative to the background using tissue phantoms with a pump-connected tube (high) and phantom spheres (low). Furthermore, in vivo validation of extracting spatiotemporal three-dimensional blood flow distributions and hyperemic response during forearm cuff occlusion is demonstrated. Finally, the success of instrument feasibility in clinical use is examined through intraoperative imaging of mastectomy skin flap.

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Section: Methodsmentioning

confidence: 99%

Noncontact 3-D Speckle Contrast Diffuse Correlation Tomography of Tissue Blood Flow Distribution

Huang

Irwin

Zhao

et al. 2017

IEEE Trans. Med. Imaging

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“…More recently, some other new optical technologies have been developed for deep-tissue blood perfusion analysis based on diffuse laser speckles [31,32]. The correction of CCD noise is also studied [32].…”

Section: Diffuse Speckle Contrast Analysismentioning

confidence: 99%

Optical methods for blood perfusion measurement—theoretical comparison among four different modalities

Dong

Poh

et al. 2015

J. Opt. Soc. Am. A

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Blood perfusion in human tissue can be measured in vivo by means of various optical methods, which seem to be very different from one another. The most prominent examples of them are laser Doppler flowmetry, laser speckle contrast imaging, diffuse correlation spectroscopy, and the most recently developed diffuse speckle contrast analysis. In this paper, we claim that these four seemingly different modalities are examining different aspects of the same entity-the temporal autocorrelation function of scattered photons. We will show how the observables in each modality can be theoretically derived from the temporal autocorrelation function, and will discuss the merits and drawbacks of each modality in its practical use.

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“…Diffuse speckle contrast analysis and speckle contrast optical spectroscopy approach the problem by using the relationship between a speckle contrast parameter and DCS theory. 8,9 Speckle contrast optical tomography (SCOT) extends the concept using an analytical Born approximation in the inverse problem on transmissionbased measurement of parallel-plane tissue phantoms. 10 We move forward from these studies in the direction of tomographic imaging by developing a robust technique combining the benefits of CCD detection and our FEMbased DCT flow reconstruction.…”

Section: Introductionmentioning

confidence: 99%

“…Speckle contrast contributions due to hardware noise (i.e., shot noise and dark noise) are accounted for correcting the speckle contrast computation, K meas = (σ/µ), of windowed regions. 9,14 Details of these corrections can be found elsewhere. 9 Briefly, dark images are used to mitigate speckle contrast influences from dark noise, I c = I − I D , where I is the original intensity of a single pixel and I D is the intensity of dark current.…”

mentioning

confidence: 99%

“…9,14 Details of these corrections can be found elsewhere. 9 Briefly, dark images are used to mitigate speckle contrast influences from dark noise, I c = I − I D , where I is the original intensity of a single pixel and I D is the intensity of dark current. The shot noise of I C follows Poisson statistics, σ s (I C ) =  µ(I C ), which are incorporated into the correction calculation.…”

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confidence: 99%

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Speckle contrast diffuse correlation tomography of complex turbid medium flow

et al. 2015

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Purpose: Developed herein is a three-dimensional (3D) flow contrast imaging system leveraging advancements in the extension of laser speckle contrast imaging theories to deep tissues along with our recently developed finite-element diffuse correlation tomography (DCT) reconstruction scheme. This technique, termed speckle contrast diffuse correlation tomography (scDCT), enables incorporation of complex optical property heterogeneities and sample boundaries. When combined with a reflectance-based design, this system facilitates a rapid segue into flow contrast imaging of larger, in vivo applications such as humans. Methods: A highly sensitive CCD camera was integrated into a reflectance-based optical system. Four long-coherence laser source positions were coupled to an optical switch for sequencing of tomographic data acquisition providing multiple projections through the sample. This system was investigated through incorporation of liquid and solid tissue-like phantoms exhibiting optical properties and flow characteristics typical of human tissues. Computer simulations were also performed for comparisons. A uniquely encountered smear correction algorithm was employed to correct pointsource illumination contributions during image capture with the frame-transfer CCD and reflectance setup. Results: Measurements with scDCT on a homogeneous liquid phantom showed that speckle contrastbased deep flow indices were within 12% of those from standard DCT. Inclusion of a solid phantom submerged below the liquid phantom surface allowed for heterogeneity detection and validation. The heterogeneity was identified successfully by reconstructed 3D flow contrast tomography with scDCT. The heterogeneity center and dimensions and averaged relative flow (within 3%) and localization were in agreement with actuality and computer simulations, respectively. Conclusions: A custom cost-effective CCD-based reflectance 3D flow imaging system demonstrated rapid acquisition of dense boundary data and, with further studies, a high potential for translatability to real tissues with arbitrary boundaries. A requisite correction was also found for measurements in the fashion of scDCT to recover accurate speckle contrast of deep tissues. C 2015 American Association of Physicists in Medicine. [http://dx

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Speckle contrast optical spectroscopy, a non-invasive, diffuse optical method for measuring microvascular blood flow in tissue

Cited by 139 publications

References 28 publications

Noncontact 3-D Speckle Contrast Diffuse Correlation Tomography of Tissue Blood Flow Distribution

Noncontact 3-D Speckle Contrast Diffuse Correlation Tomography of Tissue Blood Flow Distribution

Optical methods for blood perfusion measurement—theoretical comparison among four different modalities

Speckle contrast diffuse correlation tomography of complex turbid medium flow

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