Speckle-microscopy for blood flow measurements in the smallest microvessel

Ulyanov, Sergey S.; Ganilova, Yulia

doi:10.1117/12.500259

Cited by 1 publication

(1 citation statement)

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“…The whole mice scale 3D imaging with acceptable depth resolution of 0.1 mm fills the gap between the conventional 2D microscopic and macroscopic imaging, which enables the studies of the whole-body pathology models. With the large volume of information, the system provides capability of analyzing the microscopic details of macroscopic region of interest in the context of the intact body 28 , such as the transport of cancer cells in blood vessels 29 . High speed in vivo volumetric imaging with high contrast and high resolution offers superior advantages compared with the current tissue transparency techniques used to avoid scattering effects in imaging the details of fixed organs.…”

Section: Discussionmentioning

confidence: 99%

Deep learning enhanced NIR-II volumetric imaging of whole mice vasculature

Wu¹,

Yang²,

Ma³

et al. 2023

OEA

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Fluorescence imaging through the second near-infrared window (NIR-II,1000-1700 nm) allows in-depth imaging. However, current imaging systems use wide-field illumination and can only provide low-contrast 2D information, without depth resolution. Here, we systematically apply a light-sheet illumination, a time-gated detection, and a deep-learning algorithm to yield high-contrast high-resolution volumetric images. To achieve a large FoV (field of view) and minimize the scattering effect, we generate a light sheet as thin as 100.5 μm with a Rayleigh length of 8 mm to yield an axial resolution of 220 μm. To further suppress the background, we time-gate to only detect long lifetime luminescence achieving a high contrast of up to 0.45 Ι contrast . To enhance the resolution, we develop an algorithm based on profile protrusions detection and a deep neural network and distinguish vasculature from a low-contrast area of 0.07 Ι contrast to resolve the 100 μm small vessels. The system can rapidly scan a volume of view of 75 × 55 × 20 mm 3 and collect 750 images within 6 mins. By adding a scattering-based modality to acquire the 3D surface profile of the mice skin, we reveal the whole volumetric vasculature network with clear depth resolution within more than 1 mm from the skin. High-contrast large-scale 3D animal imaging helps us expand a new dimension in NIR-II imaging.

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Section: Discussionmentioning

confidence: 99%