Spectinomycin, gentamicin, and routine disc diffusion testing: An alternative for the treatment and monitoring of multidrug-resistant Neisseria gonorrhoeae?

Martins, Jéssica Motta; Scheffer, Mara Cristina; Machado, Hanalydia de Melo; Schörner, Marcos André; Golfetto, Lisléia; Santos, Thais Matos; Barazzetti, Fernando Hartmann; Albuquerque, Victor Cavadas Barreto de; Bazzo, Maria Luiza

doi:10.1016/j.mimet.2022.106480

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…The disk diffusion test was performed according to CLSI M02-A12 (2015) for bacteria [ 22 ]. Sterile Petri dishes (90 mm Ø) were used using 25 mL of medium.…”

Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory and Antimicrobial Activities of Portuguese Prunus avium L. (Sweet Cherry) By-Products Extracts

et al. 2022

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The bioactivity of natural by-products in food and pharmaceutical applications is the subject of numerous studies. Cherry production and processing generates large amounts of biowaste, most of which is not used. The recovery of these by-products is essential for promoting the circular economy and to improving sustainability in the food industry. In this work, we explored the anti-inflammatory and antimicrobial potential of two different extracts from stems, leaves, and flowers of Portuguese cherries. The anti-inflammatory potential was studied on lipopolysaccharide (LPS)-stimulated mouse macrophages (RAW 264.7) by evaluating the effect of by-products on cellular viability and nitric oxide (NO) production. Disc diffusion and minimum inhibitory concentration (MIC) were used to determine antimicrobial activity. The cherry by-products had no cytotoxic effect on RAW 264.7 cells, and were able to inhibit nitrite production in a dose-dependent manner. Moreover, all aqueous infusions showed good antioxidant activity against NO radicals. Moreover, leaf extracts showed the best activity against most of the strains studied. The results revealed, for the first time, interesting anti-inflammatory and antimicrobial properties of cherry by-products. This could potentially be of interest for their therapeutic use in the treatment of inflammation-related diseases or in controlling the growth of microorganisms.

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“…The disk diffusion test was performed according to CLSI M02-A12 (2015) for bacteria [ 22 ]. Sterile Petri dishes (90 mm Ø) were used using 25 mL of medium.…”

Section: Methodsmentioning

confidence: 99%

Anti-Inflammatory and Antimicrobial Activities of Portuguese Prunus avium L. (Sweet Cherry) By-Products Extracts

et al. 2022

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“…Disc diffusion is also a simpler and cheaper method that has comparable accuracy to agar dilution. 24,26,27 Therefore, this method can be used as an alternative in resource-limited settings, such as in Indonesia. 26,27…”

Section: Discussionmentioning

confidence: 99%

“…24,26,27 Therefore, this method can be used as an alternative in resource-limited settings, such as in Indonesia. 26,27…”

Section: Discussionmentioning

confidence: 99%

Susceptibility of Neisseria gonorrhoeae against a dual treatment antibiotics regimen in primary health centres in Surabaya, Indonesia

Hidayati,

Sawitri,

Rositawati

et al. 2024

Aust J Gen Pract

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also showed that gonorrhoea represented 4.1% of new sexually transmitted infection (STI) cases from 2013 to 2015. 2 The management and control of gonorrhoea has been complicated by the emergence of antimicrobial resistance (AMR) in Neisseria gonorrhoeae. 3,4 In the 1930s, sulphonamides were the first effective antibiotics for gonorrhoea, but resistance developed within the decade. In the 1940s, penicillin became the drug of choice for gonorrhoea. However, chromosomal-mediated resistance rapidly developed, resulting in a more than 100-fold increment in the penicillin dose required to cure gonorrhoea. In the 1970s, the emergence of penicillinase-producing N. gonorrhoeae (PPNG) rendered penicillin ineffective. Similarly, the introduction of tetracycline, spectinomycin and fluoroquinolones to treat gonorrhoea was followed by widespread resistance, leading to their discontinuation as drugs of choice. 5,6 In the 1990s, third generation cephalosporins, ceftriaxone and cefixime, were introduced for gonorrhoea. In 2010, due to the decreasing susceptibility of N. gonorrhoeae against these antimicrobials and frequent coinfection with chlamydia, a dual treatment regimen with an increased dose of cephalosporins and either doxycycline or azithromycin was recommended to curb the resistance to cephalosporins. [4][5][6][7] Nevertheless, the minimum inhibitory concentrations (MICs) of the constituents of this regimen gradually increased. In 2017-18, the WHO reported 0-21% gonococci isolates with reduced susceptibility (RS) to ceftriaxone, 0-22% with RS to cefixime and 0-60% with resistance to azithromycin worldwide. 3 In 2019, there were 97.1% gonococci isolates resistant to tetracycline but none with RS to ceftriaxone in Jakarta, Indonesia. 8 In 2021, the Australian Gonococcal Surveillance Program reported 0.9% gonococci isolates with RS, one isolate with resistance to ceftriaxone

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Epidemiological profile and genetic resistance of Neisseria gonorrhoeae infection in women in a poor region of São Paulo, Brazil

Tavares,

Alverga,

Felix

et al. 2024

Acta Tropica

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Spectinomycin, gentamicin, and routine disc diffusion testing: An alternative for the treatment and monitoring of multidrug-resistant Neisseria gonorrhoeae?

Cited by 5 publications

References 29 publications

Anti-Inflammatory and Antimicrobial Activities of Portuguese Prunus avium L. (Sweet Cherry) By-Products Extracts

Anti-Inflammatory and Antimicrobial Activities of Portuguese Prunus avium L. (Sweet Cherry) By-Products Extracts

Susceptibility of Neisseria gonorrhoeae against a dual treatment antibiotics regimen in primary health centres in Surabaya, Indonesia

Epidemiological profile and genetic resistance of Neisseria gonorrhoeae infection in women in a poor region of São Paulo, Brazil

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