Spectral analysis and antibacterial effect of cold methanolic extract of Artemisia absinthium L.

Sultan, Muhammad H.; Moni, Sivakumar Sivagurunathan

doi:10.1590/1519-6984.264869

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…Artemisia medicinal raw Separations 2023, 10, 545 2 of 14 material is an excellent source of biologically active compounds. The aerial parts of different Artemisia plants contain high levels of volatile [10] and non-volatile bioactive substances such as phenolics [11,12], fatty acids [13,14], organic acids [15], amino acids [13], coumarins [16], lignans [17], minerals [18,19], alkaloids [20], sterols [21], tannins [22], and carotenoids [23]. Artemisia extracts have attracted more attention in the last decades and various investigations of polyphenols, and their relation to biological activities have been conducted [24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Phenolic Diversity and Antioxidant Activity of Artemisia abrotanum L. and Artemisia absinthium L. during Vegetation Stages

Saunoriūtė,

Ragažinskienė,

Ivanauskas

et al. 2023

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Over the past ten years, particular attention has been paid to Artemisia L. genus plants and their chemical composition. Artemisia abrotanum L. and Artemisia absinthium L., which are traditional medicinal, aromatic plants with a strong digestive activity that is determined by the various phytochemicals. The research aim was to determine the variation in polyphenols in the samples of different vegetation stages of two Artemisia species and to evaluate the antioxidant activity of methanolic extracts in vitro. Phytochemical analysis of the Artemisia samples was carried out using spectrophotometric methods and HPLC-PDA techniques, while antioxidant activity was determined using DPPH, ABTS, FRAP, and CUPRAC assays. Significant differences in the diversity of phenolic compounds were found among Artemisia species during vegetation stages. Chlorogenic acid, 3,5-dicaffeoylquinic acid, and rutin were predominant among the phenolic compounds. The study provides valuable insights into the composition of phenolic compounds and flavonoids in Artemisia plants at different growth stages, shedding light on their potential pharmacological effects and antioxidant activities. These findings contribute to the knowledge of the quality and safety of herbal materials derived from Artemisia species.

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Section: Introductionmentioning

confidence: 99%

Phenolic Diversity and Antioxidant Activity of Artemisia abrotanum L. and Artemisia absinthium L. during Vegetation Stages

Saunoriūtė,

Ragažinskienė,

Ivanauskas

et al. 2023

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Phytochemical profiling and in silico evaluation of Artemisia absinthium compounds targeting Leishmania N-myristoyltransferase: molecular docking, drug-likeness, and toxicity analyses

Boudou,

Belakredar,

Berkane

et al. 2024

Front. Chem.

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BackgroundArtemisia absinthium has long been recognized for its therapeutic properties against various diseases. Among these is leishmaniasis, a parasitic infection that remains a global health challenge. Targeting Leishmania N-myristoyltransferase (NMT), a crucial enzyme for parasite survival, represents a promising therapeutic approach. The bioactive compounds in A. absinthium could potentially inhibit NMT and serve as new treatment options for leishmaniasis.AimThis study aims to investigate the phytochemical composition, drug-likeness, and molecular dynamics of A. absinthium bioactive compounds targeting Leishmania NMT, identifying potent inhibitors that could serve as new drug candidates.MethodThe extract of A. absinthium was analyzed using High-Performance Liquid Chromatography (HPLC), identifying nine phenolic compounds, with kaempferol (10.72%) and chlorogenic acid (4.43%) being the most abundant. Drug-likeness and toxicity were evaluated using SwissADME and OSIRIS Property Explorer, focusing on adherence to Lipinski’s rule of five and Ghose’s filter. Molecular docking studies were conducted to evaluate the binding affinity of these compounds to NMT. Molecular dynamics (MD) simulations were performed to assess the stability and flexibility of the NMT-apigenin complex.ResultsMolecular docking identified apigenin as the most potent NMT inhibitor, with a binding energy of −9.6 kcal/mol, forming significant hydrogen bonds with threonine residues 203 and 189. Drug-likeness analysis revealed that most compounds adhered to Lipinski’s rule of five, indicating favorable pharmacokinetic properties. MD simulations confirmed the stability of the NMT-apigenin complex, with root mean square deviation (RMSD) values of 0.04 nm, root mean square fluctuation (RMSF) values between 0.05 and 0.35 nm, and radius of gyration (Rg) values ranging from 2.24 to 2.30 nm. Normal mode analysis further supported the complex’s stability and flexibility.ConclusionThe findings of this study underscore the potential of Artemisia absinthium compounds, particularly apigenin, as promising candidates for the development of new anti-leishmaniasis drugs. The potent inhibition of Leishmania NMT by apigenin, along with its favorable pharmacokinetic and stability profiles, supports its further exploration in antileishmanial drug development.

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Spectral analysis and antibacterial effect of cold methanolic extract of Artemisia absinthium L.

Cited by 2 publications

References 19 publications

Phenolic Diversity and Antioxidant Activity of Artemisia abrotanum L. and Artemisia absinthium L. during Vegetation Stages

Phenolic Diversity and Antioxidant Activity of Artemisia abrotanum L. and Artemisia absinthium L. during Vegetation Stages

Phytochemical profiling and in silico evaluation of Artemisia absinthium compounds targeting Leishmania N-myristoyltransferase: molecular docking, drug-likeness, and toxicity analyses

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