Spectral monitoring of photoirradiated skin lipids: MS and IR approaches

Merle, Chloé; Laugel, Cécile; Baillet‐Guffroy, Arlette

doi:10.1016/j.chemphyslip.2008.03.011

Cited by 9 publications

(22 citation statements)

References 24 publications

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“…Numerous methods to qualitatively and quantitatively analyze skin tissue have been reported based on HPLC-MS, GC-MS, FTIR and NMR (Norlén et al , 1998; Merle et al , 2008; Robosky et al , 2008; Abaffy et al , 2010). The above methods usually require sample preprocessing, such as extraction, lyophilization and derivatization, and often require large amounts of sample.…”

Section: Discussionmentioning

confidence: 99%

Direct Analysis in Real Time Mass Spectrometry (DART-MS) Analysis of Skin Metabolome Changes in the Ultraviolet B-Induced Mice

Park

Kim

Jang

et al. 2013

Biomolecules and Therapeutics

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Ultraviolet (UV) radiation is a major environmental factor that leads to acute and chronic reactions in the human skin. UV exposure induces wrinkle formation, DNA damage, and generation of reactive oxygen species (ROS). Most mechanistic studies of skin physiology and pharmacology related with UV-irradiated skin have focused on proteins and their related gene expression or single- targeted small molecules. The present study identified and analyzed the alteration of skin metabolites following UVB irradiation and topical retinyl palmitate (RP, 5%) treatment in hairless mice using direct analysis in real time (DART) time-of-flight mass spectrometry (TOF-MS) with multivariate analysis. Under the negative ion mode, the DART ion source successfully ionized various fatty acids including palmitoleic and linolenic acid. From DART-TOF-MS fingerprints measured in positive mode, the prominent dehydrated ion peak (m/z: 369, M+H-H2O) of cholesterol was characterized in all three groups. In positive mode, the discrimination among three groups was much clearer than that in negative mode by using multivariate analysis of orthogonal partial-least squares-discriminant analysis (OPLS-DA). DART-TOF-MS can ionize various small organic molecules in living tissues and is an efficient alternative analytical tool for acquiring full chemical fingerprints from living tissues without requiring sample preparation. DART-MS measurement of skin tissue with multivariate analysis proved to be a powerful method to discriminate between experimental groups and to find biomarkers for various experiment models in skin dermatological research.

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Section: Discussionmentioning

confidence: 99%

Direct Analysis in Real Time Mass Spectrometry (DART-MS) Analysis of Skin Metabolome Changes in the Ultraviolet B-Induced Mice

Park

Kim

Jang

et al. 2013

Biomolecules and Therapeutics

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“…These molecules, especially the unsaturated lipids, are sensitive to UV exposure (4). The effects of UV on lipids have been widely studied (5–7) and three major consequences were found: (1) β scission (fragmentation of the carbon chain), (2) hydrogenation of the double bond of unsaturated compounds and (3) formation of oxygenated entities from unsaturated lipids.…”

Section: Introductionmentioning

confidence: 99%

“…This work deals with the correlation between the formation of new entities due to UV exposure and the supramolecular organization of the SC lipids. A previous work has already described the UVA effects on the crystalline order of the lipids but only a small part focused on the oxygenated entities formed (7). In this article, the transformation of lipids through UV irradiation is extensively studied, by mass spectrometry (MS) and IR spectroscopy.…”

Section: Introductionmentioning

confidence: 99%

“…Previous work showed that after UVA irradiation at 60 or 120 J cm −2 applied to lipid films, despite a small decrease in the packing, the different lipid classes remain globally organized in an orthorhombic state (7). In another study (15) the effect on lipid films of UVA irradiation at 120 J cm −2 was compared to UVB exposures at 0.15 and 1.5 J cm −2 .…”

Section: Introductionmentioning

confidence: 99%

“…The term “film” is widely used when lipids are auto‐associated into a liquid‐crystal organization (orthorhombic or hexagonal phases). These lamellar phases are characterized at a molecular level throughout the article from the frequencies of IR absorption bands classically used to describe the lipid organization (7,17).…”

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confidence: 99%

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Effect of UVA or UVB Irradiation on Cutaneous Lipids in Films or in Solution

Merle¹,

Laugel

Baillet‐Guffroy

2010

Photochem & Photobiology

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The barrier function of the skin is largely due to the stratum corneum which is essentially composed of lipids. Different external factors, such as UV irradiation, affect this skin layer and are responsible for a destabilization of the supramolecular organization of its constituted lipids. In this work, mass spectrometry and infrared spectroscopy are combined to study the correlation between the formation of oxidative compounds by UV irradiation and the lipid organization. Experiments were carried out on unsaturated lipids in film or solution form, exposed to UVA or UVB irradiation. UV exposure leads to the formation of oxygenated entities in the case of lipids with an unsaturated fatty acid moiety, resulting in a decrease in their packing which is greater when the lipids are in solution. The packing decrease is even greater following UVB irradiation.

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Characterization of triglycerides photooxidation under solar radiations: A stepwise Raman study

Assi

Michael‐Jubeli

Jacques

et al. 2023

Analytical Science Advances

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Triglycerides (TGs) are one of the main components of the glycerolipid family. Their main task in cells is to store excess fatty acids. TG energy storage is mainly concentrated in adipocytes. TGs and free fatty acids constitute the majority (57.5%) of the skin surface lipids (SSLs). TGs are essential for the formation of the skin water barrier. This work is the second part of a global study that aims to evaluate the effect of solar radiations on SSLs using vibrational spectroscopy. In the first part of this work, a stepwise characterization of free fatty acids was performed, and different spectral descriptors were used to follow the different structural modifications during the photo‐oxidation process, that is hydrogen abstraction, formation of hydroperoxides and peroxyl radicals as primary oxidation products and the formation of aldehydes, ketones, alcohol as secondary products. In this second part, the photo‐oxidation of TGs was evaluated using Raman spectroscopy. A decrease in the CH2/CH3 stretching bands ratio that confirmed the hydrogen abstraction, an increase in the 1165/1740 cm−1 ((δ(OH) and υ(C–O))/ν(C=O) (ester)) ratio indicated the formation of secondary oxidation products such as hydroperoxides. And finally, an increase in the 1725/1740 cm−1 (υ(C=O) (ald.)/υ(C=O) (ester)) ratio and the trans ν(C=C)/cis ν(C=C) ratio highlighted the formation of aldehydes, alcohols, ketone, trans secondary products and others.

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Spectral monitoring of photoirradiated skin lipids: MS and IR approaches

Cited by 9 publications

References 24 publications

Direct Analysis in Real Time Mass Spectrometry (DART-MS) Analysis of Skin Metabolome Changes in the Ultraviolet B-Induced Mice

Direct Analysis in Real Time Mass Spectrometry (DART-MS) Analysis of Skin Metabolome Changes in the Ultraviolet B-Induced Mice

Effect of UVA or UVB Irradiation on Cutaneous Lipids in Films or in Solution

Characterization of triglycerides photooxidation under solar radiations: A stepwise Raman study

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