Spectrin oxidation correlates with membrane vesiculation in stored RBCs

Wagner, G.; Chiu, DT; Qju, JH; Heath, RH; Lubin, B

doi:10.1182/blood.v69.6.1777.1777

Cited by 91 publications

(11 citation statements)

References 23 publications

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“…Vesiculation and formation of spheroechinocytes are also related to spectrin shortening 27 . The increase of spectrin oxidation during the storage of blood is associated with vesiculation 28 .…”

Section: Discussionmentioning

confidence: 99%

Transformation of membrane nanosurface of red blood cells under hemin action

Козлова

Черныш

Moroz

et al. 2014

Sci Rep

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Hemin is the product of hemoglobin oxidation. Some diseases may lead to a formation of hemin. The accumulation of hemin causes destruction of red blood cells (RBC) membranes. In this study the process of development of topological defects of RBC membranes within the size range from nanoscale to microscale levels is shown. The formation of the grain-like structures in the membrane (“grains”) with typical sizes of 120–200 nm was experimentally shown. The process of formation of “grains” was dependent on the hemin concentration and incubation time. The possible mechanism of membrane nanostructure alterations is proposed. The kinetic equations of formation and transformation of small and medium topological defects were analyzed. This research can be used to study the cell intoxication and analyze the action of various agents on RBC membranes.

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Section: Discussionmentioning

confidence: 99%

Transformation of membrane nanosurface of red blood cells under hemin action

Козлова

Черныш

Moroz

et al. 2014

Sci Rep

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“…ROS levels increase gradually for the first week of storage and then rapidly increase to a maximum by the second week of storage (D'Alessandro et al, 2012 ). Oxidation is shown to reduce the spectrin–actin interactions during storage (Wolfe et al, 1986 ) and correlates with vesicle release (Wagner et al, 1987 ). Aggregation of the mobile pool of band 3 coincides with increased ROS and oxidation of the RBCs (Kriebardis et al, 2007a ; Karon et al, 2012 ; Arashiki et al, 2013 ) and occurs before vesiculation (Karon et al, 2009 ).…”

Section: The Order Of the Storage Lesionmentioning

confidence: 99%

The involvement of cation leaks in the storage lesion of red blood cells

2014

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Stored blood components are a critical life-saving tool provided to patients by health services worldwide. Red cells may be stored for up to 42 days, allowing for efficient blood bank inventory management, but with prolonged storage comes an unwanted side-effect known as the “storage lesion”, which has been implicated in poorer patient outcomes. This lesion is comprised of a number of processes that are inter-dependent. Metabolic changes include a reduction in glycolysis and ATP production after the first week of storage. This leads to an accumulation of lactate and drop in pH. Longer term damage may be done by the consequent reduction in anti-oxidant enzymes, which contributes to protein and lipid oxidation via reactive oxygen species. The oxidative damage to the cytoskeleton and membrane is involved in increased vesiculation and loss of cation gradients across the membrane. The irreversible damage caused by extensive membrane loss via vesiculation alongside dehydration is likely to result in immediate splenic sequestration of these dense, spherocytic cells. Although often overlooked in the literature, the loss of the cation gradient in stored cells will be considered in more depth in this review as well as the possible effects it may have on other elements of the storage lesion. It has now become clear that blood donors can exhibit quite large variations in the properties of their red cells, including microvesicle production and the rate of cation leak. The implications for the quality of stored red cells from such donors is discussed.

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“…Lipid peroxidation disturbs phospholipid asymmetry of RBC membrane, and this leads to the externalization of phosphatidylserine (PS) to the outer leaflet of the membrane and facilitates RBC adhesion to endothelial cells [ 3 ]. Oxidative stress is a possible mechanism contributing to microvesicle formation during storage and therefore causes loss of membrane deformability [ 12 ]. Moreover, a significant decrease in RBC deformability after 2 to 4 weeks of storage was shown in several studies by different methods [ 13–17 ].…”

Section: Introductionmentioning

confidence: 99%

Blood storage alters mechanical stress responses of erythrocytes

Uğurel

Kucuksumer

Eglenen

et al. 2017

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BACKGROUND: Erythrocytes undergo irreversible morphological and biochemical changes during storage. Reduced levels of deformability have been reported for stored erythrocytes. Erythrocyte deformability is essential for healthy microcirculation.OBJECTIVE: The aim of this study is to evaluate shear stress (SS) induced improvements of erythrocyte deformability in stored blood.METHODS: Deformability changes were evaluated by applying physiological levels of SS (5 and 10 Pa) in metabolically depleted blood for 48 hours and stored blood for 35 days with citrate phosphate dextrose adenine-1 (CPDA-1). Laser diffractometry was used to measure erythrocyte deformability before and after application of SS.RESULTS: Erythrocyte deformability, as a response to continuous SS, was significantly improved in metabolically depleted blood, whereas it was significantly impaired in the blood stored for 35 days with CPDA-1 (p≤0.05). The SS-induced improvements of deformability were deteriorated due to storage and relatively impaired according to the storage time. However, deformability of stored blood after exposure to mechanical stress tends to increase at low levels of shear while decreasing at high SS levels.CONCLUSION: Impairment of erythrocyte deformability after storage may contribute to impairments in the recipient’s microcirculation after blood transfusion. The period of the storage should be considered to prevent microcirculatory problems and insufficient oxygen delivery to the tissues.

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Spectrin oxidation correlates with membrane vesiculation in stored RBCs

Cited by 91 publications

References 23 publications

Transformation of membrane nanosurface of red blood cells under hemin action

Transformation of membrane nanosurface of red blood cells under hemin action

The involvement of cation leaks in the storage lesion of red blood cells

Blood storage alters mechanical stress responses of erythrocytes

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