Spectrofluorimetric Determination of Certain Fluoroquinolone Through Charge Transfer Complex Formation

Du, Lei; Lin, Ai Ping; Yang, Ya Qin

doi:10.1081/al-200026694

Cited by 14 publications

(6 citation statements)

References 13 publications

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“…HPLC methods generally require complex and expensive equipment, provision for use and disposal of solvents, labor-intensive sample preparation procedure and personal skilled in chromatographic techniques. Charge-transfer spectrofluorimetry (CTF) has been found to be useful for the determination of quinolone in real samples [23][24][25][26][27] , showing several advantages such as low interference level, low detection limit, high sensitivity, good analytical selectivity, easy and less time consuming compared with the above mentioned methods. A new spectrofluorimetric method for determination of AMI, CIP, DIF, ENO, ENR, LOM, LEV, NOR, OFL and PEF was reported through CT complexation with BRO is described in this paper and having been satisfactory applied to the determination of studied drugs in commercial formulations and urine samples.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Determination of Certain Fluoroquinolones Through Charge Transfer Complex Formation

Salem¹,

Fada²,

Khater³

2007

American J. of Pharmacology and Toxicology

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A highly sensitive spectrofluorimetric method was developed for the first time, for the analysis of ten fluoroquinolones (FQs) antibacterials, namely amifloxacin (AMI), ciprofloxacin (CIP), difloxacin (DIF), enoxacin (ENO), enrofloxacin (ENR), lomefloxacin (LOM), levofloxacin (LEV), norfloxacin (NOR), ofloxacin (OFL) and pefloxacin (PEF) in their pharmaceutical dosage forms or in biological fluids through charge transfer (CT) complex formation with bromanil (BRO). The BRO was found to react with these drugs to produce stable complexes and the fluorescence intensity of the complexes was greatly enhanced. The formation of such complexes was also confirmed by ultravioletvisible measurements. The different experimental parameters that affect the fluorescence intensity were carefully studied. At the optimum reaction conditions, the drug-BRO complexes showed excitation maxima ranging from 275 to 290 nm and emission maxima ranging from 450 to 470 nm. Rectilinear calibration graphs were obtained in the concentration range 0.02 to 3.1 µg.mL -1 for the studied drugs. The method has been successfully applied to determine their pharmaceutical dosage forms with good precision and accuracy compared to official and reported methods as revealed by tand F-tests. They are also applied for the determination of studied drugs in human urine samples.

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Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Determination of Certain Fluoroquinolones Through Charge Transfer Complex Formation

Salem¹,

Fada²,

Khater³

2007

American J. of Pharmacology and Toxicology

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“…The majority of infrared measurements on such CT complexes have been concerned with the shifts in the vibrational frequencies of donors or acceptors. Decreases in the vibration frequency of a particular band have been used as evidence for a particular site of a CT interaction [26] . The infrared spectra of the complexes show some difference compared with the sum of the spectra of the two components.…”

Section: Effect Of Ct Reagentmentioning

confidence: 99%

“…HPLC methods generally require complex and expensive equipment, provision for use and disposal of solvents, labor-intensive sample preparation procedure and personal skilled in chromatographic techniques. Charge-transfer spectrofluorimetry (CTF) has been found to be useful for the determination of quinolone in real samples (23)(24)(25)(26)(27), showing several advantages such as low interference level, low detection limit, high sensitivity, good analytical selectivity, easy and less time consuming comparing with the above methods. A new spectrofluorimetric method for determination of AMI, CIP, DIF, ENO, ENR, LOM, LEV, NOR, OFL and PEF was reported through CT complexation with TFQ in this paper, having been satisfactory applied to the determination of studied drugs in commercial formulations and urine samples.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric Study of the Charge-transfer Complexation of Certain Fluoroquinolones with 2,3,5,6-tetrafluoro-p-bezoquinone

Geffken¹,

Salem²

2006

American J. of Applied Sciences

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A highly sensitive spectrofluorimetric method was developed for the first time, for the analysis of ten fluoroquinolones (FQs) antibacterials, namely amifloxacin (AMI), ciprofloxacin (CIP), difloxacin (DIF), enoxacin (ENO), enrofloxacin (ENR), lomefloxacin (LOM), levofloxacin (LEV), norfloxacin (NOR), ofloxacin (OFL) and pefloxacin (PEF) in their pharmaceutical dosage forms or in biological fluids through charge transfer (CT) complex formation with fluoranil (TFQ). The TFQ was found to react with these drugs to produce stable complexes and the fluorescence intensity of the complexes was greatly enhanced. The formation of such complexes was also confirmed by both infrared and ultraviolet-visible measurements.The different experimental parameters that affect the fluorescence intensity were carefully studied. At the optimum reaction conditions, the drug-TFQ complexes showed excitation maxima ranging from 270 to 285 nm and emission maxima ranging from 450 to 460 nm. Rectilinear calibration graphs were obtained in the concentration range 0.02 to 3.1 µg mL -1 for the studied drugs. The method has been successfully applied to determine their pharmaceutical dosage forms with good precision and accuracy compared to official and reported methods as revealed by t-and F-tests. They also applied for the determination of studied drugs in human urine samples.

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“…Several techniques like voltammetry [ 4 ], flow injection electrogenerated chemiluminescence [ 5 ], spectrofluorometry [ 6 , 7 ], spectrophotometry [ 8 , 9 ], high-performance liquid chromatography [ 10 , 11 ], and liquid chromatography tandem mass spectrometry [ 12 , 13 ] have been used for the determination of fluoroquinolones in pharmaceutical and biological products. Among them, spectrophotometric method has several advantages such as simplicity, fast, and low cost.…”

Section: Introductionmentioning

confidence: 99%

“…Spectrophotometry was successfully used for pharmaceutical analysis, involving quality control of commercialized product and pharmacodynamic studies. Spectrophotometric methods for the determination of fluoroquinolones could be classified according to the different reactions: (i) charge-transfer complexation based on the reaction of FQs as electron donors with p-acceptors such as 2,3-dichloro-5,6-dicyano-q-benzoquinone, 7,7,8,8-tetracyanoquinodimethane, q-chloranil, q-nitrophenol, and tetracyanoethylene [ 7 , 14 – 16 ]; (ii) oxidative coupling reaction using oxidative coupling with 3-methyl-2-benzothiazolinonehydrazone hydrochloride and cerium (IV) ammonium sulfate, Fe(III)-MBTH, tris( o -phenanthroline) iron(II), and tris (bipyridyl) iron(II) [ 17 , 18 ]; (iii) ion-pair complex formation with acid-dye reagents such as Sudan III, methyl orange, supracene violet 3B, tropaeolin 000, bromophenol blue, bromothymol blue, bromocresol green, and bromocresol purple [ 8 , 14 , 19 , 20 ]. These methods were related with some major drawbacks such as having narrow linearity range, requiring heating and close pH control, long time for the reaction to complete, and low stability of the colored product formed.…”

Section: Introductionmentioning

confidence: 99%

Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue

Dung

Dong

et al. 2018

Journal of Analytical Methods in Chemistry

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In this paper, we reported a new, simple, accurate, and precise extractive spectrophotometric method for the determination of fluoroquinolones (FQs) including ciprofloxacin (CFX), levofloxacin (LFX), and ofloxacin (OFX) in pharmaceutical formulations. The proposed method is based on the ion-pair formation complexes between FQs and an anionic dye, bromothymol blue (BTB), in acidic medium. The yellow-colored complexes which were extracted into chloroform were measured at the wavelengths of 420, 415, and 418 nm for CFX, LFX, and OFX, respectively. Some effective conditions such as pH, dye concentration, shaking time, and organic solvents were also systematically studied. Very good limit of detection (LOD) of 0.084 µg/mL, 0.101 µg/mL, and 0.105 µg/mL were found for CFX, LFX, and OFX, respectively. The stoichiometry of the complexes formed between FQs and BTB determined by Job's method of continuous variation was 1 : 1. No interference was observed from common excipients occurred in pharmaceutical formulations. The proposed method has been successfully applied to determine the FQs in some pharmaceutical products. A good agreement between extractive spectrophotometric method with high-performance liquid chromatography mass spectrometry (HPLC-MS) for the determination of FQs in some real samples demonstrates that the proposed method is suitable to quantify FQs in pharmaceutical formulations.

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Spectrofluorimetric Determination of Certain Fluoroquinolone Through Charge Transfer Complex Formation

Cited by 14 publications

References 13 publications

Spectrofluorimetric Determination of Certain Fluoroquinolones Through Charge Transfer Complex Formation

Spectrofluorimetric Determination of Certain Fluoroquinolones Through Charge Transfer Complex Formation

Spectrofluorimetric Study of the Charge-transfer Complexation of Certain Fluoroquinolones with 2,3,5,6-tetrafluoro-p-bezoquinone

Determination of Fluoroquinolones in Pharmaceutical Formulations by Extractive Spectrophotometric Methods Using Ion-Pair Complex Formation with Bromothymol Blue

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