Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence

Moussa, Bahia Abbas; Mahrouse, Marianne Alphonse; Hassan, Mahmoud Ali; Fawzy, Michael Gamal

doi:10.2478/acph-2014-0005

Cited by 20 publications

(9 citation statements)

References 15 publications

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“…Linezolid can be determined by HPLC-UV [22], UHPLC-PDA [23], quinone-based fluorophores [24], and LC-MS-MS [25]. HPLC-UV and LC-MS-MS are two widely used methods in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

UPLC-MS/MS Determination of Linezolid and Heme in Plasma of Infected Patients and Correlation Analysis

Wang

Lan

et al. 2021

BioMed Research International

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Linezolid can cause serious haematological toxicity, such as thrombocytopenia and aneamia. Heme, composed of iron and porphyrin, is an important component of hemoglobin. In order to investigate the relationship between the concentration of linezolid and heme in the plasma of infected patients, a UPLC-MS/MS method that can determine the concentrations of linezolid and heme simultaneously was developed and validated. A total of 96 healthy subjects and 81 infected patients, who received blood routine blood tests, were included and determined by the UPLC-MS/MS method. The results showed that the concentration of linezolid was 5.08 ± 3.46 μ g / mL in infected patients who were treated with linezolid. The heme in healthy subjects was 7.05 ± 8.68 μ g / mL , and it was significantly decreased to 0.88 ± 0.79 μ g / mL in infected patients ( P < 0.01 ). Spearman correlation analysis showed that linezolid had a high negative correlation with platelet (PLT) ( R = − 0.309 ). Heme had a high positive correlation with hemoglobin (Hb) ( R = 0.249 ) in healthy subjects and infected patients. The ROC analysis showed that heme had diagnostic value to distinguish low Hb (110 g/L). In conclusion, there was a positive correlation between heme and Hb, and this correlation was also observed in infected patients. A high concentration of linezolid was inclined to decrease PLT. Monitoring of heme and linezolid helps in the early diagnose of low Hb and PLT.

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“…Linezolid can be determined by HPLC-UV [22], UHPLC-PDA [23], quinone-based fluorophores [24], and LC-MS-MS [25]. HPLC-UV and LC-MS-MS are two widely used methods in clinical practice.…”

Section: Discussionmentioning

confidence: 99%

UPLC-MS/MS Determination of Linezolid and Heme in Plasma of Infected Patients and Correlation Analysis

Wang

Lan

et al. 2021

BioMed Research International

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“…Forced degradation conditions of the drugs were based on the above ICH guidelines in addition to different previously reported researches. [33][34][35][36][37] The light effect is considered an important factor in the stability of drugs. Great problems of stability arise by the light of the shorter and longer wavelength, in particular from ultraviolet and visible.…”

Section: Forced Degradation Studymentioning

confidence: 99%

TLC-spectrodensitometric method for simultaneous determination of dapagliflozin and rosuvastatin in rabbit plasma: stability indicating assay and kinetic studies

et al. 2020

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“…It is widely used for treatment of respiratory infections (bronchitis, pneumonia) and urinary infections. A few analytical techniques have been constructed for the quantification of Gemifloxacin in pure forms, such as, Spectrophotometric method [25][26] , HPTLC 27 , RP-HPLC [28][29] , LC-MS 30 , UV-Spectrofluorometric method 31 and Zone electrophoresis 32 .…”

Section: Gemifloxacin Chemically Is [{Rs}7-[{4z}-3-{aminomethyl}-mentioning

confidence: 99%

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2019

IJPSR

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Simple, sensitive and selective methods are developed for the spectrophotometric determination of drugs, viz., Cetirizine hydrochloride, Duloxetine hydrochloride, Esmolol hydrochloride, Gemifloxacin, Moxifloxacin and Phenylephrine Hydrochloride based on their reactivity towards Chloramine-T (ChL-T). The method is based on the oxidation of drugs by ChL-T (Excess amount) in presence of acidic medium and estimating the amount of un-reacted ChL-T by Safranin-O (SO) dye at λmax 524 nm. The amount of ChL-T reacted is equal to the drug concentration in this method. The concentration of ChL-T is 10 µgmL-1 and Safranin-O Dye is 10 µgmL-1. The calibration curves obeyed Beer's law over the concentration range of 2.5-17.5 µgmL-1 (CET), 5-35 µgmL-1 (DUL), 12-84 µgmL-1 (ESM), 10-70 µgmL-1 (GEM) & 2.5-17.5 µgmL-1 (MOX). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of International Conference on Harmonization. INTRODUCTION: Cetirizine dihydrochloride chemically is [2-[4-[(4-chlorophenyl) phenyl methyl]-1 piperazinyl] ethoxy] acetic acid di HCl. Cetirizine di HCl is belonged to the group of the second-generation h1 antagonist, inhibits allergic reaction mediated by histamine.

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Spectrofluorimetric determination of gemifloxacin mesylate and linezolid in pharmaceutical formulations: Application of quinone-based fluorophores and enhanced native fluorescence

Cited by 20 publications

References 15 publications

UPLC-MS/MS Determination of Linezolid and Heme in Plasma of Infected Patients and Correlation Analysis

UPLC-MS/MS Determination of Linezolid and Heme in Plasma of Infected Patients and Correlation Analysis

TLC-spectrodensitometric method for simultaneous determination of dapagliflozin and rosuvastatin in rabbit plasma: stability indicating assay and kinetic studies

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