Spectrofluorimetric method for the determination of piroxicam and pyridoxine

Escandar, Graciela M.; Bystol, Adam J.; Campiglia, Andrés D.

doi:10.1016/s0003-2670(02)00595-0

Cited by 62 publications

(32 citation statements)

References 29 publications

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“…According to labels of used gel and tablets the artificial real sample was prepared in the same proportion as that of a commercial formulation. 31 The results for both analytes are shown in Table 4. Good recovery results for both drugs show that excipients do not interfere in the determination of PX and PY.…”

Section: Real Samplementioning

confidence: 99%

“…Among B complex vitamins, pyridoxine, PY, (2-methyl-3-hydroxy-4,5-bis(hydroxyl-methyl)-pyridine (Scheme 1)) is one of the members of the vitamin B6 group, and possesses a significant native fluorescence in solution, which often interferes in the spectrofluorimetric determination of PX. 31 Because PY is essential for both protein and red blood cell metabolism, 32 several methods have been developed for its determination, including fluorescent ones. 33,34 Escander et al 31 determined PX and PY in pharmaceutical preparations by fluorimetry after separating them by solid-phase extraction.…”

Section: Introductionmentioning

confidence: 99%

“…31 Because PY is essential for both protein and red blood cell metabolism, 32 several methods have been developed for its determination, including fluorescent ones. 33,34 Escander et al 31 determined PX and PY in pharmaceutical preparations by fluorimetry after separating them by solid-phase extraction. To the best of our knowledge, there is not any report about spectrofluorimetric simultaneous determination of these two compounds in pharmaceutical preparations without separation.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

2006

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One of the most serious problems that can occur in classical quantitative analysis is the presence of one or more spectral interferent-chemical species which affect the instrument response and which are unaccounted for in the calibration process. Advances in chemometric methods allow quantitative analysis in the presence of unidentified interferents if a threeway experimental data matrix is available for each sample. This property is the so-called "second-order advantage" 1 and it is based on the earlier work done in the psychometrics field. 2,3 The use of this advantage in analytical chemistry was proposed by Ho et al. 4 (rank annihilation factor analysis, RAFA), for the multicomponent analysis of fluorescent mixtures using excitation-emission matrix (EEM). Later, Lorber 5 and Sanchez and Kowalski 6 proposed new and simple solutions for the method of Ho et al. and now it is called the generalized rank annihilation method (GRAM).At present, analytical chemistry laboratories have instrumentation that easily generates multidimensional data structures of experimental data for each sample. Fluorescence is a particularly interesting technique because it allows, in a very straightforward manner, the acquisition of this type of information, of which the most typical example is the excitation-emission matrix (EEM). Several algorithms that use second-order data are described in the literature. [7][8][9] Of these, the generalized rank annihilation method (GRAM) 6,11,12 has interesting properties since it only requires two data matrices for quantification: one from the calibration sample and the other from the unknown sample. Recently, it has been proved that GRAM predicts results that are similar to those from the parallel factor analysis (PARAFAC) when only two samples are used, 9 so both methods can be useful as calibration methods for second-order data. But, unlike PARAFAC, GRAM is a non-iterative method which tries to implement the second-order advantage. Second-order calibration using GRAM has several distinct advantages. 13The most pronounced advantage is that it makes possible the quantification of the analyte(s) of interest in samples containing interferents. Those interferents can be completely unknown, that is, it is not necessary that they have been incorporated in the standards used for calibration. Another advantage is that it is possible to recover the individual instrument responses of the analyte(s) of interest, e.g., the excitation and emission spectra can be obtained for the individual analyte(s). This makes it possible to perform qualitative analysis and to check the identity of the analytes. The Kowalski group has proposed the trilinear decomposition (TLD) method. 14,15 In this work we focused our attention on the ability of GRAM for analyzing the second-order data.Fluorescence spectroscopy is a versatile tool mainly used because of its selectivity and sensitivity. Fluorescence spectra can be recorded in difference modes such as emission, excitation, synchronous and excitation-emission matrix (EEM). T...

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Section: Real Samplementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

2006

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“…Several methods have been reported for the assay of NSAIDs in pharmaceuticals and biological fluids; in general these works deal with a single compound or a few compounds, and employ capillary electrophoresis [10][11][12][13][14][15], spectrofluorimetry [16][17][18][19][20], flow injection fluorimetry [21] or gas chromatography coupled to mass spectrometry (GC/MS) [22][23][24]. GC methods are timeconsuming and have been largely replaced by HPLC methods with ultra-violet (UV) [25][26][27][28][29][30][31][32][33][34][35] detection, electrochemical detection [36], or mass spectrometry [37].…”

Section: Introductionmentioning

confidence: 99%

Determination of non-steroidal anti-inflammatory drugs in pharmaceuticals and human serum by dual-mode gradient HPLC and fluorescence detection

Ibrahim

Boyer

Bouajila

et al. 2007

Journal of Chromatography B

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“…Different analytical techniques have been described for PX assaying in pharmaceutical formulations, including UV-Vis spectrophotometry [3][4][5][6][7], spectrofluorimetry [8][9][10], flow injection spectrophotometry [11], voltammetry [12,13], capillary zone electrophoresis [14] and HPLC [15,16]. Stability indicating chromatographic methods [17][18][19][20] have been reported for PX determination with special interest to check for the potential impurities.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorimetric determination of 2-aminopyridine as a potential impurity in piroxicam and tenoxicam within the pharmacopoeial limit

Barary

Abdel-Hay

Sabry

et al. 2004

Journal of Pharmaceutical and Biomedical Analysis

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Spectrofluorimetric method for the determination of piroxicam and pyridoxine

Cited by 62 publications

References 29 publications

Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

Simultaneous Spectrofluorometric Determination of Piroxicam and Pyridoxine Using Generalized Rank Annihilation Method

Determination of non-steroidal anti-inflammatory drugs in pharmaceuticals and human serum by dual-mode gradient HPLC and fluorescence detection

Spectrofluorimetric determination of 2-aminopyridine as a potential impurity in piroxicam and tenoxicam within the pharmacopoeial limit

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