Spectrofluorimetric Studies of the Interaction Between Quinine Sulfate and Riboflavin

Patil, D. T.; Bhattar, S. L.; Kolekar, Govind B.; Patil, Shivajirao R.

doi:10.1007/s10953-010-9643-5

Cited by 15 publications

(12 citation statements)

References 24 publications

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“…The above spectral observation led us to consider that FRET occurs between PVP‐ANPs and FL Na + in aqueous solution. The quenching results fit into the well known Stern–Volmer relationship .

\frac{F_{0}}{F} = 1 + k_{q} τ_{0} ([], Q) = 1 + K_{S V} ([], Q)

where F o and F are the fluorescence intensities of the donor (PVP‐ANPs) in the absence and in the presence of the acceptor (FL Na + ) respectively; K q , K SV , τ o and [Q] are the concentration of fluorescein sodium, the Stern‐Volmer quenching constant, the average fluorescence lifetime of PVP‐ANPs without FL Na + and the concentration of FL Na + respectively.…”

Section: Resultsmentioning

confidence: 94%

Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples

et al. 2015

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Based on the known complexation ability between polyvinyl pyrrolidone (PVP) and fluorescein sodium (FL Na(+)), fluorescent PVP capped anthracene nanoparticles (PVP-ANPs) were prepared using a reprecipitation method for detection of fluorescein in aqueous solution using the fluorescence resonance energy transfer (FRET) approach. A dynamic light scattering histogram of PVP-ANPs showed narrower particle size distribution and the average particle size was 15 nm. The aggregation-induced enhanced emission (AIEE) of PVP-ANPs was red shifted from its monomer by 1087.22 cm(-1). The maximum emission was seen to occur at 420 nm. The presence of FL Na(+) in the vicinity of PVP-ANPs quenched the fluorescence of PVP-ANPs because of its adsorption on the surface of PVP-ANPs in aqueous suspension. The FL Na(+) and PVP-ANPs were brought close enough, typically to 7.89 nm, which was less than the distance of 10 nm that is required between the energy donor-acceptor molecule for efficient FRET. The quenching results fit into the Stern-Volmer relationship even at temperatures greater than ambient temperatures. The thermodynamic parameters determined from FRET results helped to propose binding mechanisms involving hydrophobic and electrostatic molecular interaction. The fluorescence quenching results were used further to develop an analytical method for estimation of fluorescein sodium from ophthalmic samples available commercially in the market.

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\frac{F_{0}}{F} = 1 + k_{q} τ_{0} ([], Q) = 1 + K_{S V} ([], Q)

Section: Resultsmentioning

confidence: 94%

Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples

et al. 2015

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“…However, the slope and binding constant obtained are 1.0085 and 1.99 Â 10 5 l mol À1 respectively. Since the slope provides the number of bound ligand is equal to one, we intend that one ligand binds per molecule of nanoparticles and the binding constant value indicating that a strong dye nanoparticles interaction [19][20][21].…”

Section: Kinetics Of Quenching Of Agnps Fluorescencementioning

confidence: 99%

Evaluation of interparticle interaction between colloidal Ag nanoparticles coated with trisodium citrate and safranine by using FRET: Spectroscopic and mechanistic approach

Mokashi

Gore

Sudarsan

et al. 2012

Journal of Photochemistry and Photobiology B: Biology

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“…It has a high fluorescence quantum yield on account of its circular structure. Owing to the medical applications and its use as fluorescence standard, the photophysics of QS was found to be the topic of broad research in the past . Photophysical behavior of Trp and QS designates significant overlap between emission spectrum of Trp with excitation spectrum of QS.…”

Section: Introductionmentioning

confidence: 99%

An Investigation of Fluorescence Resonance Energy Transfer between Tryptophan and Quinine Sulphate

Mahanwar

Patil

Naik

et al. 2019

Macromolecular Symposia

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Fluorescence resonance energy transfer (FRET) between tryptophan (Trp) and quinine sulphate (QS) has been investigated in aqueous medium at room temperature by absorption, emission, and time resolved fluorescence spectroscopy. Absorbance spectral characteristics rule out the possibility of ground state complex formation between them. Time resolved decay indicates dynamic type of quenching. Saturation of efficiency of energy transfer reveals the existence of FRET. Efficiency of energy transfer and distance between donor and acceptor pair were determined according to Förster's non radiative energy transfer theory.

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Spectrofluorimetric Studies of the Interaction Between Quinine Sulfate and Riboflavin

Cited by 15 publications

References 24 publications

Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples

Polyvinyl pyrrolidone capped fluorescent anthracene nanoparticles for sensing fluorescein sodium in aqueous solution and analytical application for ophthalmic samples

Evaluation of interparticle interaction between colloidal Ag nanoparticles coated with trisodium citrate and safranine by using FRET: Spectroscopic and mechanistic approach

An Investigation of Fluorescence Resonance Energy Transfer between Tryptophan and Quinine Sulphate

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