Spectrofluorometric and HPLC Determinations of Ribavirin in Capsules Based on Fluorescence Derivatization of the Sugar Moiety

Abstract

Simple and sensitive spectrofluorometric and HPLC methods for the determination of ribavirin (RIB) were developed. The methods were based on the reaction of the 5′-hydroxyl group of the sugar moiety in RIB with dansyl chloride in a bicarbonate solution (pH 10.5) to form a fluorescent derivative. The first method was based on measuring the fluorescence intensity of the derivative in dichloromethane at 529 nm (excitation at 382 nm). The second method was HPLC separation of the derivative on a reversed-phase C18 column with fluorescence detection. The linear ranges were 200 -900 and 50 -1000 ng/mL for the spectrofluorometric and HPLC methods, respectively. The derivatization product was characterized by spectroscopic methods. The proposed methods were successfully applied to analysis of the capsules.

Design and Fabrication of a Biomimetic Nanochannel for Highly Sensitive Arginine Response in Serum Samples

“…Firstly, the Arg derivation of the α‐amine group with 5‐(dimethylamino)naphthalene‐1‐sulfonyl chloride (DNS‐Cl) was synthesized in bicarbonate solution (pH 10.4), as it has strong and stable fluorescent signals. This process followed the reported procedure 21. Then, a PET membrane modified with C4AH was soaked in 1 m M DNS‐Arg solutions for 1 h, allowing the reaction to take place between C4AH and Arg.…”

“…This process followed the reported procedure. [21] Then, a PET membrane modified with C4AH was soaked in 1 mm DNS-Arg solutions for 1 h, allowing the reaction to take place between C4AH and Arg. The fluorescent signal was observed after washing the PET membrane with deionized water several times (Figure 9).…”

Validated Stability-Indicating Liquid Chromatographic Method for the Determination of Ribavirin in the Presence of Its Degradation Products: Application to Degradation Kinetics

“…Later on different analytical methods were reported for the determination of ribavirin either in pure form or in pharmaceutical preparations and biological fluids. These methods include spectrofluorimetry (8,9), HPLC (10 -13) and IR (14). None of the reported methods were described as a stability-indicating method for the determination of ribavirin except for a TLC method (15); however, this reported method did not introduce a degradation kinetic study.…”

Chiral recognition of Arg based on label-free PET nanochannel

“…3B) focused on the inner membrane surface. We used an Arg fluorescent derivative (Arg-F), which was synthesized by derivatization of the a-amine group with 5-(dimethylamino)naphthalene-1-sulfonyl chloride (DNS-Cl) 19 (Fig. 3A) to interact with BSA.…”