Spectrofluorometric Determination of Putrescine Using Orthophthaldehyde as a Fluorophore: A Novel Method

Abstract

This paper concerns spectrofluorometric analysis of putrescine using orthophthaladehyde as a fluorophore in aqueous alkaline medium. Wavelengths of excitation and emission in acid, neutral, and alkaline media were different. There is a maximum intensity of fluorescence in alkaline medium 24 h after complexation compared with other media. Putrescine and orthophthaladehyde are used at an equimolar ratio, and the product is kinetically stable in alkaline medium. Calibration curves obtained gave limits of detection and quantification of 39 and 65 ng/mL, respectively. The correlation coefficient obtained in alkaline medium was 0.992 at pH 12. Results obtained largely showed a good reproducibility of our method.

Orthophthaldehyde as a fluorescent probe for the feasible and sensitive assay of heptaminol: application to dosage forms and real human plasma

“…Chemical derivatization using o-phthaladehyde (OPA) in the presence of 2-mercapto-ethanol (2-ME) has been widely used for the determination of several compounds containing primary amine groups, including biogenic amines [21,22] and pharmaceutical compounds. [23][24][25] Isoindole is a reaction product with high fluorescence activity.…”