Spectrophotometric analysis evaluating apical microleakage in retrograde filling using GIC, MTA and biodentine: an in-vitro study

Nepal, Manisha; Shubham, Snigdha; Tripathi, Rupam; Khadka, Jwolan; Kunwar, Deepa; Gautam, Vanita; Gautam, Narayan

doi:10.1186/s12903-020-1025-9

Cited by 21 publications

(13 citation statements)

References 25 publications

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“…As part of modern endodontic surgery protocols, a variety of retrograde filling materials are used, such as intermediate restorative material (IRM) [3], mineral trioxide aggregate (MTA) [4], and Biodentine [4].…”

Section: Introductionmentioning

confidence: 99%

The Invasion of Bacterial Biofilms into the Dentinal Tubules of Extracted Teeth Retrofilled with Fluorescently Labeled Retrograde Filling Materials

et al. 2020

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In this study, we evaluated the invasion of bacteria into the dentinal tubules of retrofilled extracted human teeth, and the influence of different fluorescently labeled retrograde filling materials on the bacterial invasion and viability, by means of confocal laser scanning microscopy (CLSM). The root apices of extracted teeth were cut, prepared, and filled retrogradely using either intermediate restorative material (IRM), mineral trioxide aggregate (MTA), or Biodentine. The roots were filled with Enterococcus faecalis bacteria from their coronal part for 21 days. Then, 3-mm-long apical segments were cut to get root axial slices, and the bacteria were fluorescently stained and evaluated by CLSM. Bacterial penetration into the dentinal tubules favored the bucco-lingual directions. The filling materials penetrated up to 957 µm into the tubuli, and the bacteria, up to 1480 µm (means: 130 and 167 μm, respectively). Biodentine fillings penetrated less and the associated bacteria penetrated deeper into the tubuli compared to MTA or IRM (p = 0.004). Deeper filling penetration was associated with shallower penetration of both dead and live, or live alone, bacteria (p = 0.015). In conclusion, the current study enables better understanding of the microbiological–pathological course after endodontic surgical procedures. It was found that even with retrograde fillings, bacteria invade deep into the dental tubules, where deeper filling penetration prevents deeper penetration of the bacteria and adversely affects the viability of the bacteria.

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Section: Introductionmentioning

confidence: 99%

The Invasion of Bacterial Biofilms into the Dentinal Tubules of Extracted Teeth Retrofilled with Fluorescently Labeled Retrograde Filling Materials

et al. 2020

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“…Both materials used in this study as apical plugs showed similar attachment of osteoblast cells on their surfaces [18] and significant antimicrobial effect against E. faecalis [19]. In addition, they showed good marginal adaptation and similar sealing ability [20,21]. The thickness of the apical plug plays an important role in maintaining an adequate apical seal.…”

Section: Discussionmentioning

confidence: 86%

Comparison of MTA versus Biodentine in Apexification Procedure for Nonvital Immature First Permanent Molars: A Randomized Clinical Trial

et al. 2022

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This study aimed to evaluate the radiological and clinical outcomes of Biodentine apical plugs compared to mineral trioxide aggregate (MTA) in treating immature molars with apical lesions in children. Materials and Methods: Thirty immature roots of 24 permanent lower first molars with apical lesions were randomly divided into two groups: group 1 (15 roots) treated with MTA apical plugs and group 2 (15 roots) treated with Biodentine apical plugs. Treatment radiological outcomes were assessed using the periapical index (PAI) scale after 6 and 12 months of treatment. The presence or absence of apical calcified barrier (ACB) was assessed after 12 months of treatment. The visual analog scale (VAS) was used to compare the postoperative pain between the two groups after 1, 3, 7, and 14 days of treatment. PAI scores between the two groups were compared using the Mann–Whitney U test, the presence or absence of the ACB was compared using the chi-square test, and the VAS scores were compared using the t-test. The statistical significance threshold was set at 0.05. Results: There were no statistically significant differences in the PAI between the two groups at 6 and 12 months postoperatively. After 12 months, four cases in the Biodentine group showed ACB formation, whereas ACB was not found in any case treated with MTA. The VAS scores were statistically lower in the MTA group on the first day after treatment. Nevertheless, these scores were not statistically significantly different after 3, 7, and 14 days of treatment between the two groups. Conclusions: Biodentine can be used as an apical plug to treat immature permanent molars with apical lesions in a single visit in children. Biodentine showed favorable outcomes in apical lesions healing, which was comparable to MTA but with a decreased treatment time associated with its use.

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“…Contrarily, the dye extraction technique used in this study recovers all of the dye that leaked through the apex by dissolving it in acid, thus avoiding the drawbacks of sectioning the root. It also quantitatively measures the optical density of the solution by using a spectrophotometer, thus producing accurate results for the microleakage studies [46,47]. The lowest microleakage was reported for the endodontic pressure syringe group, with significant difference with that of the modified disposable syringe and the reamer technique.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Different Obturation Techniques in Primary Teeth on the Apical Microleakage using Endoflas: A Comparative In Vitro Study

Irzooqee

Haidar

Abdul-Kareem

2023

International Journal of Dentistry

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Objectives. This study was carried out to quantitatively evaluate and compare the sealing ability of Endoflas by using different obturation techniques. Materials and Methods. After 42 extracted primary maxillary incisors and canines were decoronated, their canals were instrumented with K files of size ranging from #15 to #50. In accordance with the obturation technique, the samples were divided into three experimental groups, namely, group I: endodontic pressure syringe, group II: modified disposable syringe, and group III: reamer technique, and two control groups. Dye extraction method was used for leakage evaluation. Data were analyzed using one-way ANOVA and Dunnett’s T3 post hoc tests. The level of significance was set at p < 0.05 . Results. Endodontic pressure syringe had significantly less leakage than the modified disposable syringe and reamer techniques ( p < 0.05 ). Meanwhile, no significant difference was found in the mean leakage between the modified disposable syringe and the reamer techniques. Conclusion. Amongst all the techniques used in this study, endodontic pressure syringe could be preferred as an obturation technique in primary teeth when used with Endoflas obturation material because of its potential to provide good apical seal.

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Spectrophotometric analysis evaluating apical microleakage in retrograde filling using GIC, MTA and biodentine: an in-vitro study

Cited by 21 publications

References 25 publications

The Invasion of Bacterial Biofilms into the Dentinal Tubules of Extracted Teeth Retrofilled with Fluorescently Labeled Retrograde Filling Materials

The Invasion of Bacterial Biofilms into the Dentinal Tubules of Extracted Teeth Retrofilled with Fluorescently Labeled Retrograde Filling Materials

Comparison of MTA versus Biodentine in Apexification Procedure for Nonvital Immature First Permanent Molars: A Randomized Clinical Trial

The Effect of Different Obturation Techniques in Primary Teeth on the Apical Microleakage using Endoflas: A Comparative In Vitro Study

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