Spectrophotometric analysis of bioactive metabolites and fermentation optimisation of
            <i>Streptomyces</i>
            sp. HU2014 with antifungal potential against
            <i>Rhizoctonia solani</i>

Zhu, Hongxia; Hu, Linfeng; Рожкова, Т. О.; Wang, Xinfa; Li, Chengwei

doi:10.1080/13102818.2023.2178822

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…A difference in the nature of molecules produced by Streptomyces sp. HU2014 was observed by spectrophotometric analyses of cell-free filtrate (CFF) after different incubation times [ 47 ]. Indeed, it has been described that Streptomyce s strains arborized a complex life cycle, which is closely related to specialized metabolite production [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, it has been described that Streptomyce s strains arborized a complex life cycle, which is closely related to specialized metabolite production [ 48 ]. The bioactive molecules produced depend on both culture conditions and strains [ 47 , 49 , 50 , 51 ]. Moreover, according to Campos-Avelar et al (2021), among the 59 Streptomyces isolates tested, all of them can reduce AFB1 concentration in co-culture with A. flavus .…”

Section: Discussionmentioning

confidence: 99%

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Effect of Streptomyces roseolus Cell-Free Supernatants on the Fungal Development, Transcriptome, and Aflatoxin B1 Production of Aspergillus flavus

Maud

Boyer

Durrieu

et al. 2023

Toxins

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Crop contamination by aflatoxin B1 (AFB1), an Aspergillus-flavus-produced toxin, is frequently observed in tropical and subtropical regions. This phenomenon is emerging in Europe, most likely as a result of climate change. Alternative methods, such as biocontrol agents (BCAs), are currently being developed to reduce the use of chemicals in the prevention of mycotoxin contamination. Actinobacteria are known to produce many bioactive compounds, and some of them can reduce in vitro AFB1 concentration. In this context, the present study aims to analyze the effect of a cell-free supernatant (CFS) from Streptomyces roseolus culture on the development of A. flavus, as well as on its transcriptome profile using microarray assay and its impact on AFB1 concentration. Results demonstrated that in vitro, the S. roseolus CFS reduced the dry weight and conidiation of A. flavus from 77% and 43%, respectively, and was therefore associated with a reduction in AFB1 concentration reduction to levels under the limit of quantification. The transcriptomic data analysis revealed that 5198 genes were differentially expressed in response to the CFS exposure and among them 5169 were downregulated including most of the genes involved in biosynthetic gene clusters. The aflatoxins’ gene cluster was the most downregulated. Other gene clusters, such as the aspergillic acid, aspirochlorine, and ustiloxin B gene clusters, were also downregulated and associated with a variation in their concentration, confirmed by LC-HRMS.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of Streptomyces roseolus Cell-Free Supernatants on the Fungal Development, Transcriptome, and Aflatoxin B1 Production of Aspergillus flavus

Maud

Boyer

Durrieu

et al. 2023

Toxins

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Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12

Oskay

2024

International Journal of Secondary Metabolite

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An actinobacterium newly isolated from soil during a screening study was identified as Streptomyces sp. MC12 (GenBank accession number: PP757795) based on 16S rRNA analysis. For secondary metabolite production, fermentation was carried out in ISP 2 broth at 30°C, pH 7.3, for seven days under shaking conditions at 180 rpm. As a result of fermentation studies, the antagonistic effect of the crude extract, obtained through ethyl acetate extraction, against various microorganisms was determined. The MIC values of the extract against Staphylococcus aureus and Escherichia coli were 101.3 µg/mL and 153.6 µg/mL, respectively. It was also found to exhibit strong antifungal activity against Penicillium spp. Streptomyces sp. MC12, which displays both antifungal and antibacterial properties, is considered a potential secondary metabolite producer for future studies, particularly in pharmacology and the biocontrol of fungal pathogens.

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Biocontrol of Mycotoxigenic Fungi by Actinobacteria

Maud,

Barakat,

Bornot

et al. 2024

JoF

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Actinobacteria are well known for their production of metabolites of interest. They have been previously studied to identify new antibiotics in medical research and for their ability to stimulate plant growth in agronomic research. Actinobacteria represents a real source of potential biocontrol agents (BCAs) today. With the aim of reducing the use of phytosanitary products by 50% with the different Ecophyto plans, a possible application is the fight against mycotoxin-producing fungi in food matrices and crops using BCAs. To deal with this problem, the use of actinobacteria, notably belonging to the Streptomyces genus, or their specialized metabolites seems to be a solution. In this review, we focused on the impact of actinobacteria or their metabolites on the development of mycotoxigenic fungi and mycotoxin production on the one hand, and on the other hand on their ability to detoxify food matrices contaminated by mycotoxins.

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Spectrophotometric analysis of bioactive metabolites and fermentation optimisation of Streptomyces sp. HU2014 with antifungal potential against Rhizoctonia solani

Cited by 3 publications

References 41 publications

Effect of Streptomyces roseolus Cell-Free Supernatants on the Fungal Development, Transcriptome, and Aflatoxin B1 Production of Aspergillus flavus

Effect of Streptomyces roseolus Cell-Free Supernatants on the Fungal Development, Transcriptome, and Aflatoxin B1 Production of Aspergillus flavus

Taxonomic characterization and secondary metabolite production of newly isolated Streptomyces sp. MC12

Biocontrol of Mycotoxigenic Fungi by Actinobacteria

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