Spectrophotometric and fluorometric assay of superoxide ion using 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole

Olojo, Rotimi; Xia, Ruohong; Abramson, Jonathan J.

doi:10.1016/j.ab.2005.01.032

Cited by 117 publications

(62 citation statements)

References 24 publications

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“…The product of reaction of NBD-Cl with superoxide generates a new absorption maximum at 470 nm. Of particular note, hydrogen peroxide, NAD(P)H and NAD + were reported not to interfere with this reaction [33].…”

Section: Spectrophotometric Probesmentioning

confidence: 99%

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Use of spectroscopic probes for detection of reactive oxygen species

Bartosz

2006

Clinica Chimica Acta

271

174

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Section: Spectrophotometric Probesmentioning

confidence: 99%

“…The product of NBD-Cl reaction with superoxide shows fluorescence, albeit only in organic solvent. Therefore, fluorimetric quantification of superoxide production is possible with this probe if the sample is mixed with an organic solvent prior to measurement [33].…”

Section: Fluorescence Probesmentioning

confidence: 99%

Use of spectroscopic probes for detection of reactive oxygen species

Bartosz

2006

Clinica Chimica Acta

271

174

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“…XTT is a probe that readily reacts with superoxide (Sutherland and Learmonth, 1997). Its reduction by superoxide resulted in a time-dependent increase in absorbance at 470 nm (Olojo et al, 2005). More potent electron donors increased the rate and amount of reduction of XTT.…”

Section: Methodsmentioning

confidence: 99%

Designing Calcium Release Channel Inhibitors with Enhanced Electron Donor Properties: Stabilizing the Closed State of Ryanodine Receptor Type 1

Ye¹,

Yaeger²,

Owen³

et al. 2011

Mol Pharmacol

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New drugs with enhanced electron donor properties that target the ryanodine receptor from skeletal muscle sarcoplasmic reticulum (RyR1) are shown to be potent inhibitors of single-channel activity. In this article, we synthesize derivatives of the channel activator 4-chloro-3-methyl phenol (4-CmC) and the 1,4-benzothiazepine channel inhibitor 4-[-3{1-(4-benzyl) piperidinyl}propionyl]-7-methoxy-2,3,4,5-tetrahydro-1,4-benzothiazepine (K201, JTV519) with enhanced electron donor properties. Instead of activating channel activity (ϳ100 M), the 4-methoxy analog of 4-CmC -ATPase type 1. Moreover, the FKBP12 protein, which stabilizes RyR1 in a closed configuration, is shown to be a strong electron donor. It seems as if FKBP12, K201, its dioxole derivative, and 4-MmC inhibit RyR1 channel activity by virtue of their electron donor characteristics. These results embody strong evidence that designing new drugs to target RyR1 with enhanced electron donor characteristics results in more potent channel inhibitors. This is a novel approach to the design of new, more potent drugs with the aim of functionally modifying RyR1 single-channel activity.

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“…iii) monitoring of the gene transfection [12]; iv) fluorescent tagging of sugars for visualization of cells showing high uptake of the corresponding sugar [13][14][15]; v) derivatization of various compounds in spectrofluorimetry, chromatography and electrophoresis [16][17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%