Spectrophotometric Assay of 5,5′‐(Salicylidene)bis‐(2‐thiobarbituric acid)

Abstract

The spectrophotometric assay of 5,5'-(salicylidene)bis-(2-thiobarbituric acid), which shows interesting pharmacological perspectives, is proposed, Spektrornetrische Bestimmung der 5,5'-(Salicyliden)bis-(2-thiobarbitursaure) applying the conventional and differential photometries. The conventional photometry is developed at pH 4.00 (NaOAc-HOAc buffer). The differential photometry is realized for two series of pH (pH 10.5/13.5 and pH 2.0/10.5). A comparative study between the different photometries indicates a higher precision for the differential photometry in the event of pH 2.0/10.5.Es wird eine spektrometrische Bestimmung der 5,5'-(Salicyliden)bis-(2thiobarbitursaure), die interessante pharmakologische Perspektiven bietet, vorgeschlagen. Neben der UV-Spektrometrie bei pH = 4.0 wird die Differenzspektrometrie bei verschiedenen pH-Werten untersucht. Dabei zeigt sich die Differenzspektrometrie mit Verwendung der pH-abhangigen Absorption bei pH = 2 und pH = 10.5 den anderen Methoden uberlegen.Gravimetric and spectrophotometric methods for barbituric acids were the most utilized and accepted by organizations as AOAC, appearing as official methods in the different Pharmacopeias. At present spectrophotometric methods, together with the chromatographic ones, are used to a higher extent. They have the advantage of good accuracy and precision in general, and they are accesible, well studied and some of them are very fast methods. In this paper conventional and differential spectrophotometric determination of 5,5'-(salicylidene) bis-(2-thiobarbituric) acid (1) are proposed, which offers interesting pharmacological perspectives. The 5,5'-(salicylidene) bis-(barbituric) acid analogue has been assayed as a sympathomimetic agent on dogs, cats, rabbits and mice, showing an outstanding and long vasopressor effect; it causes an increase of the blood pressure of

Ligating Properties of 5-Nitrobarbituric Acid

“…The 250 C peak for the Mn II complex is due to dehydration of the coordinated water. The peaks at 188.2, 272 and 280.4 C for copper and manganese complexes, respectively, are probably due to 3 ] Á 5H 2 O complexes gave seven and eight peaks, respectively. The ®rst two peaks at 92.6 and 131.6 C for the cobalt complex and 85.5 and 151.0 C for the nickel complex are due to dehydration of the outer sphere water molecules.…”

“…Most pyrimidines have antimicrobial, anti-in¯ammatory and antitumor properties [2,3]. Masoud et al 5-Nitrobarbituric acid was reported for the identi®cation of 4-aminopyridine by microcrystal reaction [45].…”

Hydrogen-Bonded Networks Constructed with 5-Nitrobarbiturate

“…In the past decade, barbituric acid and its simple C5-substituted derivates have found numerous applications in medicinal chemistry. Barbituric acid, formally a cyclic urea derivative, is of particular interest in view of its pharmacological, biochemical, and biological capabilities. − For example, the thymine group of deoxyribonucleic acid (DNA) gives barbituric acid upon biological oxidation . In the present study, 5-nitrobarbituric acid (also named dilituric acid or 5-nitro-2,4,6-trihydroxypyrimidine) is selected as a structural component instead of barbituric acid because the presence of the nitro group provides additional hydrogen-bonding acceptor sites for supramolecular assembly of the host lattice.…”

Electronic Spectral Properties of Substituted Arylazothiobarbiturate Compounds in the Presence of Different Solvents

“…The therapeutic applications of thiobarbituric acid are reported [3]. Most of the pyrimidines are with antimicrobial, anti-inflammatory, and antitumoral activities properties [2,4].…”