Spectrophotometric Assay of Certain Aminoglycosides Using Cyanoacetamide

Zakhari, N. A.

doi:10.1080/00032719008052531

Cited by 20 publications

(6 citation statements)

References 11 publications

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“…In the interest of using this kind of antibiotic scientifically and securely, finding a safe range, an optimum dosage and reasonable intervals, and establishing an individualized therapy, it is very important to develop an accurate and quick examination technique for antibiotic serum concentration. Nowadays, the primary methods for the determination of aminoglycoside antibiotics include microbiological assay (MA) [2,3], high-performance liquid chromatography (HPLC) [4,5], ultraviolet visual spectrophotometry (UV-VIS) [6,7], and immune assay (IA) [8,9]. However, MA is too trivial and time consuming to satisfy the need for a quick determination with severely injured and emergency patients, so it is being gradually phased out.…”

Section: Introductionmentioning

confidence: 99%

Resonance Rayleigh scattering spectra for studying the interaction of aminoglycoside antibiotics with pontamine sky blue and their analytical applications

Hu¹,

Liu²,

Li³

2003

Anal Bioanal Chem

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In a weakly acid medium, some aminoglycoside antibiotics, such as kanamycin (KANA), gentamicin (GEN), tobramycin (TOB), and neomycin (NEO), or acid bisazo dye pontamine sky blue (PSB) can only produce very weak resonance Rayleigh scattering (RRS) signals. However, when the two agents react with each other to form the ion association complexes, the RRS intensity can be enhanced greatly and a new RRS spectrum and a significant enhancement of the RRS intensity in the wavelength range 350-600 nm can be observed. The maximum scattering peak is at 580 nm. There is a linear relationship between the RRS intensity and the antibiotic concentration in the range 0.01-6.0 microg mL(-1) at 580 nm. This RRS method has therefore been developed for the determination of trace levels of aminoglycoside antibiotics. The detection limits (3 sigma) of the four antibiotics, whose order of sensitivity is KANA>NEO>TOB>GEN, are 5.8-6.9 ng mL(-1). This method has a good selectivity and has been successfully applied to the quick determination of antibiotics not only for injections and ear drops, but clinic serum samples as well. In addition, quantum chemistry-based analysis of the reaction mechanism, the factors influencing the RRS spectra, and the reasons for the enhancement of RRS are discussed.

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Section: Introductionmentioning

confidence: 99%

Resonance Rayleigh scattering spectra for studying the interaction of aminoglycoside antibiotics with pontamine sky blue and their analytical applications

Hu¹,

Liu²,

Li³

2003

Anal Bioanal Chem

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“…Few fluorometric methods were published for the determination of cephalexin, based on the reaction with acetyl acetoneformaldhyde, [19] fluorescamine, [20] or measurement of fluorescence intensity after acid hydrolysis and subsequent alkalinization. [21] 2-Cyanoacetamide (2-CAA) is a fluorogenic reagent; this reagent was used in post -column derivatization in HPLC determinations of catecholamines [22] and carbohydrates [23] and also used for the fluorometric determination of some important pharmaceutical compounds such as prenalterol HCl, [24] oxamniquine, [25] ascorbic acid, [26] aminoglycosides, [27] and 3,4-dihydroxyphenylalanine. [28] In the current work, 2-CAA is used for the fluorometric determination of cephalexin in presence of 33% ammonia solution.…”

Section: Introductionmentioning

confidence: 99%

Spectrofluorometric Determination of Cephalexin in Pharmaceutical Preparations and Spiked Human Urine Using 2‐Cyanoacetamide

El‐Wasseef

2007

Spectroscopy Letters

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A simple, accurate, sensitive, and validated method was developed for the spectrofluorometric determination of cephalexin. The method involves the reaction of cephalexin with 2-cyanoacetamide in presence of 33% ammonia solution. The formed fluorescent product exhibited maximum fluorescence intensity at l 439 nm, after excitation at l 339 nm. Different experimental parameters affecting the derivatization reaction were carefully studied and incorporated in the procedure. Under the described conditions, the proposed method was linear over the concentration range 0.04-0.4 mg/ mL. The average percent found was 99.6 + 0.9%. The LOD was 7.76 ng/mL. The proposed method was applied for determination of cephalexin in pharmaceutical preparations as well as in spiked human urine. A mechanism of the reaction is postulated.

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“…These advantages make them important clinical antibacterial drugs. The determination of aminoglycoside antibiotics by UV-Vis has been previously based on the formation of a complex with metal ions [14] or on the color reaction with organic reagents [15], with sensitivities between 1.8×10 3 and 1.8×10 4 l mol -1 cm -1 . However, MA is too trivial, time-consuming and expensive to satisfy rapid determination.…”

Section: Introductionmentioning

confidence: 99%

Study on the interaction of aminoglycoside antibiotics with Titan Yellow by spectrophotometric method and their analytical applications

et al. 2006

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In a weak acidic medium, the reaction of some aminoglycoside antibiotics (AGs) such as kanamycin sulfate (KANA), gentamycin sulfate (GEN), and tobramycin sulfate (TOB) with acid thiazolyl bisazo dye Titan Yellow (TY) can result in the fading of TY. The maximum fading wavelength was located at 409 nm. The molar absorptivities (ε/×10 4 l mol -1 cm -1 ) were 2.0, 1.5 and 2.5 for KANA, GEN and TOB, respectively. The spectral characteristics, effect factors, optimum conditions of the reaction and the influence of foreign substances were also investigated. The procedure is easy and fast. This method has high sensitivity and can be applied to the determination of commercial aminoglycoside antibiotics and serum samples with satisfactory results.

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Spectrophotometric Assay of Certain Aminoglycosides Using Cyanoacetamide

Cited by 20 publications

References 11 publications

Resonance Rayleigh scattering spectra for studying the interaction of aminoglycoside antibiotics with pontamine sky blue and their analytical applications

Resonance Rayleigh scattering spectra for studying the interaction of aminoglycoside antibiotics with pontamine sky blue and their analytical applications

Spectrofluorometric Determination of Cephalexin in Pharmaceutical Preparations and Spiked Human Urine Using 2‐Cyanoacetamide

Study on the interaction of aminoglycoside antibiotics with Titan Yellow by spectrophotometric method and their analytical applications

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