Spectrophotometric determination of a substance trifusol in a veterinary suppository

Abstract

The purpose of the work was to develop of a method for the quantitative determination of piperidinium 2-[5-(2-furyl)-4-phenyl-1,2,4triazol-3-ylthio] acetate (trifuzol) as part of a dosage form -an effervescent intrauterine suppository using spectrophotometry in the ultraviolet region spectrum and its validation according to State Pharmacopoeia of Ukraine. Materials and methods.The study was used a working standard sample of trifuzol, intrauterine effervescent veterinary suppositories, 1.0 g of trifuzol, as a solvent -purified water. Analytical equipment: Specord 200 spectrophotometer, electronic scales ABT-120-5DM, ultrasonic bath ELMASONICE 60 H, class A measuring dishes. Method of spectrophotometric analysis was used.Results. A spectrophotometric method has been developed and validated for the quantitative determination of trifuzol in a dosage form -an intrauterine effervescent veterinary suppository, based on measuring the absorption of an aqueous solution of the drug at 278 nm.The methodology tally the requirements of State Pharmacopoeia of Ukraine for such validation characteristics as specificity, linearity, precision, correctness, and robustness. The analysis of the predicted total uncertainty of the analysis was showed the reproducibilityof the method and the possibility of its application in other laboratories. Conclusions.A method for the quantitative determination of trifuzol in the composition of the dosage form, an effervescent intrauterine suppository, according to the requirements of State Pharmacopoeia of Ukraine, was developed and validated. It was proved that according to such validation characteristics as linearity, specificity, precision, correctness, and robustness, the technique is correct.