Spectrophotometric Determination of Acetylsalicylic Acid, Paracetamol and Ascorbic Acid by Chemometric Methods

Abstract

Acetylsalicylic acid, paracetamol, and ascorbic acid which are used for the pain reliever and fever reducer were analyzed without any prior reservation by means of spectrophotometry-chemometry. The experimental calibration matrix was designed by measuring the absorbance within the range of 200-400 nm for 19 samples of acetylsalicylic acid, paracetamol, and ascorbic acid. Absorbance and concentration values were analyzed using Minitab and other chemometric programs to calculate estimated concentrations by PCR and PLSR. In the first step the synthetic mixtures including acetylsalicylic acid, paracetamol, and ascorbic acid were prepared and absorbance values were obtained according to spectrophotometry. In the second step, amounts of acetylsalicylic acid, paracetamol, and ascorbic acid were calculated in effervescent tablet (Afebryl®). Standard deviations were accomplished. This study encouraged us to apply the developed methods for drug analysis. The methods presented in this paper are rapid and do not need any separation process or preliminary treatments for the analysis.

A novel strategy on the spectrochromatographic analysis of a quaternary mixture by parallel factor analysis model

“…The assay results of PARAFAC application to the chromatographic data tensor were found to be comparable with those obtained using the classical UPLC technique. In previous studies, the PARAFAC decomposition of the UPLC data sets was not reported for determining and quantifying ASC, ACE, and ASP (Dinç, 1999; Dinç & Özdemir, 2004; Doǧan & Duran, 1998; Ertokus & Tugrul, 2018; Eskiköy Bayraktepe & Yazan, 2020; Hajianfl & Ghobadi, 2014; Wesoły, Cetó, del Valle, Ciosek, & Wróblewski, 2016). This is a novelty of this paper with the second‐order advantage that provides the individual contributions of analytes in the presence of overlapping chromatographic and spectral signals.…”

“…As in prescription medicines, the quality control and routine analysis of this multicomponent formulation is also a desired and necessary procedure in the steps before and after drug production. In previously published papers, several conventional analytical methods have been reported for the quantitative resolution of ternary mixtures containing ASC, ACE, and ASP, including spectrophotometry (Dinç, 1999; Dinç & Özdemir, 2004; Doǧan & Duran, 1998; Ertokus & Tugrul, 2018; Hajianfl & Ghobadi, 2014), electrochemical methods (Eskiköy Bayraktepe & Yazan, 2020; Wesoły et al, 2016), and conventional liquid chromatographic methods (Akay et al, 1999; Selimoğlu, Kadıoğlu, & Dinç, 2016; Thomis, Roets, & Hoogmartens, 1984).…”