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The aim of this study was to evaluate the prevalence of mycotic mastitis in different mammary gland statuses. The study was conducted on 304 dairy cows from ten farms in two north-eastern regions in Algeria; Guelma and Souk Ahras with 922 and 199 samples, respectively, forming thus a total number of 1,121 milk samples. A total of 321 milk samples were collected from clinical mastitis, 544 milk samples from subclinical mastitis and 256 milk samples from healthy mammary glands. Mycological analyses revealed that 10.17% of the treated samples were positive recording 114 species of fungi including 88 yeasts and 26 moulds. The most frequent species was Candida kefyr followed by C. albicans, C. guilliermondii, C. famata, C. tropicalis, C. colliculosa, C. krusei, C. rugosa, C. glabrata, C. parapsilosis, C. inconspicua, Trichosporon sp., Rhodotorula glutinis and Saccharomyces fragilis. Mould species have also been isolated from samples of both healthy milk and clinical mastitis milk. Aspergillus amstelodami (from glaucus group), A. fumigatus and Geotrichum candidum were identified, while the other species including Penicillium sp. and Cladosporium sp. were not identified.

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Abstract.-In a previous study, Thymus numidicus has showed a high antifungal activity. Here, GC/MS analysis was used for the chemical determination of the essential oil from T. numidicus. Samples were obtained by hydrodistillation from plants gathered in three different places during the biological cycle. Essential oils were high in phenolic monoterpens and in their precursors, and in non-phenolic oxygenated and non-oxygenated monoterpens. The arrangement of several statistical tools has enabled a relation between the chemical composition variation of essential oils during the biological cycle and the abiotic conditions of each place. Essential oils collected during the periods: end of March and end of April were low in monoterpens, high in oxygenated monoterpens and phenols. The periods corresponding to July were typical from the biological cycle end, with high level in p-cymene and low level in phenols and linalool.Key words : essential oil -GC/MS -p-cymene -thymol -Thymus numidicus.Résumé.-Dans une étude précédente, Thymus numidicus avait montré une grande activité antifongique. Ici, l'analyse CPG/SM a été utilisée pour la déter-mination chimique de l'huile essentielle de T. numidicus. Les échantillons sont obtenus par hydrodistillation des plantes cueillies dans trois sites différents pendant le cycle biologique. Les huiles essentielles présentent des teneurs élevées en monoterpènes phénoliques et en leurs précurseurs, et en monoterpènes non phénoliques oxygénés et non oxygénés. L'utilisation de plusieurs calculs statistiques a conduit à une relation entre la variation de la composition chimique des huiles essentielles obtenues pendant le cycle biologique et les conditions abiotiques de chaque site. Les huiles essentielles de la période de fin mars et de fin avril présentent une teneur faible en monoterpènes et élevée en monoterpènes oxygénés et en phénols. Les échantillons du début juin contiennent davantage de phénols. Les périodes de juillet sont caractéristiques de la fin du cycle biologique, avec une teneur élevée en p-cymène et faible en phénols et en linalol.Mots clés : huile essentielle -CPG/SM -p-cymène -thymol -Thymus numidicus.