Spectrophotometric Determination of Chlorzoxazone in pure State and Formulations Through Oxidative Coupling of its Hydrolysis Product

Sastry, C. S. P.; Chintalapati, Ravi; Sastry, Bhethanabhotla S.; Lakshmi, C. Vijaya

doi:10.1080/00032710008543205

Cited by 8 publications

(6 citation statements)

References 11 publications

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“…2,3 Various analytical methods for therapeutic monitoring have been reported in the literature for the determination of Chlorzoxazone in commercial dosage form and biological fluids such as high-performance liquid chromatography (HPLC), 4 reverse phaseÀhigh performance liquid chromatography (RP-HPLC), 5 liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), 6 and spectrophotometry. 7 The main problems encountered in using such methods are either the need for derivatization or the need for time-consuming extraction procedures.…”

Section: Introductionmentioning

confidence: 99%

Development of Electrochemical Method for the Determination of Chlorzoxazone Drug and its Analytical Applications to Pharmaceutical Dosage Form and Human Biological Fluids

Abbar

Nandibewoor

2011

Ind. Eng. Chem. Res.

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The electrochemical behavior of chlorzoxazone drug at glassy carbon electrode has been investigated for the first time using cyclic and square wave voltammetric techniques. The dependence of the current on pH, concentration, and scan rate was investigated to optimize the experimental conditions for determination of chlorzoxazone. The anodic peak was characterized and the process was diffusion-controlled. The number of electrons transferred in the oxidation process was calculated and a plausible oxidation mechanism was proposed. In the range of 8.0 × 10–7 to 1.0 × 10–5 M, the current measured by square wave voltammetry presents a good linear property as a function of the concentration of chlorzoxazone with a detection limit of 4.41 × 10–8 M with good selectivity and sensitivity. The proposed method was successfully applied to chlorzoxazone determination under physiological condition in pharmaceutical samples and for the detection of chlorzoxazone in human biological fluids. This method can be employed in clinical analysis, quality control, and routine determination of drugs in pharmaceutical formulations.

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Section: Introductionmentioning

confidence: 99%

Development of Electrochemical Method for the Determination of Chlorzoxazone Drug and its Analytical Applications to Pharmaceutical Dosage Form and Human Biological Fluids

Abbar

Nandibewoor

2011

Ind. Eng. Chem. Res.

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“…The linear range and limit of detection of the proposed method were also compared with the previously reported methods (Table 2). It is observed that the detection limit was better than in the reported HPLC method, 3 and the lower linear range was lower than in the published HPLC methods, 3,4,7,10 spectrophotometric methods, 24,32,33 capillary zone electrophoresis, 37 and the voltammetric method using the gold electrode. 39…”

Section: Analytical Performance Of the Proposed Methodsmentioning

confidence: 76%

“…It is used as an adjunct in the symptomatic treatment of painful muscle spasm. 1,2 phy, [3][4][5][6][7][8][9][10][11][12][13] thin layer chromatography, [14][15][16][17] liquid chromatography-tandem mass spectrometry, 18 packed column supercritical fluid chromatography, [19][20] gas chromatography, [21][22][23] spectrophotometry, [24][25][26][27][28][29][30][31][32][33][34][35] fluorimetry, 36 and capillary zone electrophoresis. 37 Two papers have been described in literature concerning the voltammetric determination of chlorzoxazone based on the oxidation of the drug at the glassy carbon electrode and gold electrode.…”

Section: Introductionmentioning

confidence: 99%

Differential Pulse Anodic Voltammetric Determination of Chlorzoxazone in Pharmaceutical Formulation using Carbon Paste Electrode

Zayed

Issa

2020

ACSi

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The electrochemical behavior of chlorzoxazone at the carbon paste electrode was investigated in 0.04 mol/L Britton-Robinson buffer pH 6.50 using cyclic and differential pulse voltammetric techniques. Cyclic voltammetric studies indicated that the oxidation of the drug was irreversible and controlled mainly by diffusion. Experimental and instrumental parameters were optimized (50 mV/s scan rate, 50 mV pulse amplitude, and 0.04 mol/L Britton-Robinson (BR) buffer pH 6.50 as a supporting electrolyte) and a sensitive differential pulse anodic voltammetric method has been developed for the determination of the drug over the concentration range 0.17–1.68 μg/mL chlorzoxazone, with detection and quantitation limits of 0.05 and 0.16 μg/mL, respectively. The proposed voltammetric method was successfully applied to the determinationof the drug in its pharmaceutical formulation (Myoflex tablets), and in spiked human urine samples.

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“…These methods were applied for the analysis of this compound as a related substance of chlorzoxazone or as an environmental water pollutant. Few colorimetric methods were described for the determination of ACP through coupling with diazotized sulfanilic acid 7 or through oxidative coupling with 3-methyl-2-benzothia zolinone hydrazone (MBTH) in the presence of Fe(III), N, N-dimethyl-p-phenylene diamine (DMPD) in the presence of periodate (IO 4 -), or 2, 6dichloroquinone chlorimide (DCQC) 8 . Fluorometric determination of ACP was investigated by Stewart and Chan 9 based on chemical derivatization of ACP with fluorescamine at pH 4.2 and measuring the fluorescence at 495 nm after excitation at 395 nm.…”

Section: Chlorzoxazonementioning

confidence: 99%

Untitled

2011

IJPSR

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2-amino-4-chlorophenol (ACP) is a synthetic precursor of the skeletal muscle relaxant; chlorzoxazone. A rapid simple colorimetric method was proposed for the determination of 2amino-4-chlorophenol as a potential impurity in chlorzoxazone bulk powder via its reaction with 4-aminoantipyrine in presence of alkaline oxidizing agent (K 3 [Fe(CN) 6 ] / NH 3 ) and measuring the produced red color at 520 nm. Different experimental parameters affecting the formation of the reaction product were studied. The study included the effect of volumes of the used reagents as well as the effect of time on the formation and stability of the reaction product. The formation constant (K f ) of the reaction product was found to be 1.2 x 10 4 indicating a very stable reaction product. Moreover, the Gibbs free energy change of the reaction (ΔG) was found to be -2.3 x 10 4 K.J/mole pointing out to the spontaneous nature of the reaction. The method was successfully applied for the trace analysis of the studied compound in spiked chlorzoxazone powder. The calibration graph was linear over the range of 1-20 µg/ml with average recovery of 100.58±0.89, detection limit of 0.2 µg/ml, and quantitation limit of 0.6 µg/ml. The obtained results were statistically analyzed and were in good agreement with those obtained by the official TLC method.

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Spectrophotometric Determination of Chlorzoxazone in pure State and Formulations Through Oxidative Coupling of its Hydrolysis Product

Abstract: Three simple and sensitive procedures (Methods A, B and C) for the assay of chlorzoxazone (CZZ) in pure form and in formulations are described.

Cited by 8 publications

References 11 publications

Development of Electrochemical Method for the Determination of Chlorzoxazone Drug and its Analytical Applications to Pharmaceutical Dosage Form and Human Biological Fluids

Development of Electrochemical Method for the Determination of Chlorzoxazone Drug and its Analytical Applications to Pharmaceutical Dosage Form and Human Biological Fluids

Differential Pulse Anodic Voltammetric Determination of Chlorzoxazone in Pharmaceutical Formulation using Carbon Paste Electrode

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