Spectrophotometric Determination of Humic Substances in Water by Sorption on XAD-2 Resin Followed by Desorption with Sodium Dodecyl Sulfate

Sorouradin, Mohammad-Hussein; Hiraide, Masataka; Kawaguchi, Hiroshi

doi:10.2116/analsci.9.863

Cited by 7 publications

(5 citation statements)

References 2 publications

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“…A wavelength of 400 nm is the lowermost wavelength of visible light (400-780 nm) (Lambert, Shurvell, Lightner, & Cooks, 2014). Given that 400 nm is the visible light wavelength at which the absorbance of humic acid is highest (Kumada, 1965;Sorouradin, Hiraide, & Kawaguchi, 1993), this wavelength was adopted in the study. One millilitre of the formerly prepared DBS solutions, from every triplicate, was diluted 10 times with deionized water, and the absorbance at the 400 nm UV-vis light spectrum and 222.5 nm UV light spectrum was measured using the Shimadzu UV-1208 spectrophotometer (Tokyo, Japan).…”

Section: Measurement Of Absorbance At 2225 and 400 Nmmentioning

confidence: 99%

Precise estimation of dodecylbenzenesulfonate in aqueous solution containing dissolved organic matter extracted from soil using UV‐spectrometry

Hossain

Ishiguro

2021

European J Soil Science

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Precise estimation of sodium dodecylbenzenesulfonate (DBS) is essential for understanding its adsorption in soils, transport, toxicity, fate, and its application in the remediation of contaminated soil and groundwater. DBS can be easily identified by its conjugated double bond systems of benzene rings under the ultraviolet (UV) spectrum of a spectrophotometer. However, benzene group components of dissolved organic matter (DOM) also absorb light in the UV spectrum, and this effect is responsible for the excess quantities of DBS that are falsely measured by spectrophotometers. This study was conducted to propose a method for accurately measuring the DBS concentration of a solution containing DOM. To elucidate the influence of DOM during the UVspectroscopic measurement of DBS in an aqueous solution, 222.5 nm UV and 400 nm UV-vis spectra were measured. The DOM was extracted from a highly humic non-allophanic volcanic ash (Andosol) soil by using 1 mmol NaCl/L and 100 mmol NaCl/L solutions as the extractants. The absorbances at both 222.5 and 400 nm increased in proportion to the increase in the DOM concentration. The relationship between the absorbances at the two different wavelengths could be expressed as a linear relationship. On the other hand, the absorbance at 400 nm did not increase with the increase in the DBS concentration, whereas the absorbance at 222.5 nm increased with the increase in the DOM concentration. Therefore, we inferred that the influence of DOM on the absorbance value of the DBS-DOM complex solution at 222.5 nm could be eliminated by using the linear relationship between the two different absorbances of the DBS-free DOM solution. This method makes it possible to easily measure the DBS concentration of a solution from soil water, streams or industrial effluents containing DOM, without using reagents. Highlights• Dodecylbenzenesulfonate in a solution is overestimated in the presence of dissolved organic matter.• Dodecylbenzenesulfonate becomes precisely detectable using 222.5 and 400 nm spectrophotometry.

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Section: Measurement Of Absorbance At 2225 and 400 Nmmentioning

confidence: 99%

Precise estimation of dodecylbenzenesulfonate in aqueous solution containing dissolved organic matter extracted from soil using UV‐spectrometry

Hossain

Ishiguro

2021

European J Soil Science

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“…Generally, a direct determination of HA and FA is limited by the relatively higher detection limits of existing spectrophotometric [14][15][16] and fluorescent techniques. 17,18 This problem has been approached by the application of preconcentration techniques involving, chroma-tographic methods using adsorption resins such as XAD-2 and XAD-8, DEADE-cellulose, Duolite A-7 and activated charcoal, 19,20 freeze drying or roto-evaporation, 21 liquid extraction, 22 ultrafiltration, 23 and adsorption. 24 Of the separation and preconcentration methods studied previously, the most extensively applied has been column chromatography using non-ionic XAD resins.…”

Section: Introductionmentioning

confidence: 99%

Trace humic and fulvic acid determination in natural water by cloud point extraction/preconcentration using non-ionic and cationic surfactants with FI-UV detection

Wuilloud

Sadi

et al. 2003

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A preconcentration and determination method for humic and fulvic acids at trace levels in natural water samples was developed. Cloud point extraction was successfully employed for the preconcentration of humic acid (HA) and fulvic acid (FA) prior to the determination by using a flow injection (FI) system coupled to a spectrophotometric UV-Vis detector. The quantitative extraction of HA and FA within the pH range 1-12 was obtained by neutralization of the anionic charge on the humic substances with a cationic surfactant, hexadecyltrimethylammonium bromide (CTAB). This generated a hydrophobic species that was subsequently incorporated (solubilized) into the micelles of a non-ionic surfactant polyethylene glycol, tert-octylphenyl ether (Triton X-114). The FI method for HA and FA determination was developed by injection of 100 microl of the extracted surfactant-rich phase using an HPLC pump with spectrophotometric detection at 350 nm. A 50 ml sample solution preconcentration allowed an enrichment factor of 167. The limit of detection (LOD) obtained under the optimal conditions was 5 microg l(-1). The precision for ten replicate determinations at 0.2 mg l(-1) HA was 3.1% relative standard deviation (RSD), calculated from the peak heights. The calibration using the preconcentration system for HA and FA was linear with a correlation coefficient (r2) of 0.9997 at levels near the detection limits up to at least 1 mg l(-1). The method was successfully applied to the determination of HA and FA in natural water samples (river water).

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“…However, 400 nm is the lowermost wavelength of the visible light (400-780 nm) spectra (Lambert et al, 2014). Kumada (1965) and Sorouradin et al (1993) stated that the absorbance of humic acid is highest at the 400 nm visible light spectrum when using spectrometry. We employed this 400 nm UV-vis spectrum to evaluate the opacity of the DBS-DOM complex solution as an indicator of the presence of DOM.…”

Section: Spectrometric Measurement Of Absorbances Using the Uv And Uv...mentioning

confidence: 99%

Influence of high pH state of dodecylbenzenesulfonate and dissolved organic matter complex solution on the ultraviolet spectrometry of dodecylbenzenesulfonate

Hossain

Ishiguro

2022

J Surfact & Detergents

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Dodecylbenzenesulfonate (DBS) is an anionic surfactant that is the most commonly used ingredient in modern cleaning agents. Globally, billions of liters of untreated DBS are released daily into natural water bodies, which may lead to fatal consequences in micro and macro living entities, and destroy the natural ecosystem. Therefore, a simple, precise, rapid, and inexpensive method for measuring DBS is crucial for developing countries. The conventional ultraviolet (UV) spectrophotometric method cannot accurately estimate the DBS concentration in a solution containing a considerable quantity of dissolved organic matter (DOM). Recently, a new spectrometric method was developed using the 222.5 nm UV and 400 nm UV-visible (UV-vis) spectra to precisely estimate DBS concentration in the DBS-DOM complex solution. However, this newly developed method is yet to be validated under higher pH conditions. The accuracy of DBS measurements from the DBS-DOM complex solution under lower pH (5.5 and 6.5) and higher pH (12.5) conditions was compared in this study using the previously developed method. With the higher pH, the influence of the electrolyte increased the absorbance under the 222.5 nm UV spectrum. However, such influence decreased with the increase in DBS and DOM concentration. The UV-vis absorbance at 400 nm decreased under higher pH values when the DOM concentration increased, owing to the conformational change in DOM. Despite the contrasting trends of the absorbances in the two spectra, the studied method was proven to be equally applicable and efficient, even under higher pH conditions.

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Spectrophotometric Determination of Humic Substances in Water by Sorption on XAD-2 Resin Followed by Desorption with Sodium Dodecyl Sulfate

Cited by 7 publications

References 2 publications

Precise estimation of dodecylbenzenesulfonate in aqueous solution containing dissolved organic matter extracted from soil using UV‐spectrometry

Precise estimation of dodecylbenzenesulfonate in aqueous solution containing dissolved organic matter extracted from soil using UV‐spectrometry

Trace humic and fulvic acid determination in natural water by cloud point extraction/preconcentration using non-ionic and cationic surfactants with FI-UV detection

Influence of high pH state of dodecylbenzenesulfonate and dissolved organic matter complex solution on the ultraviolet spectrometry of dodecylbenzenesulfonate

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