Spectrophotometric determination of lactic acid

Borshchevskaya, L. N.; Gordeeva, T. L.; Kalinina, A. N.; Синеокий, С. П.

doi:10.1134/s1061934816080037

Cited by 173 publications

(86 citation statements)

References 7 publications

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“…Others use the catalyzing effect of lactate oxidase enzyme along with either an electrochemical oxygen sensor [ 36 ] or a spectrophotometer [ 37 , 38 ] to build a relationship between lactate concentration and oxygen consumption. Another non-commercial spectrophotometeric method which utilizes UV/Visible region to determine lactate concentration is based on the reaction of lactate ions with iron(III) chloride at 390 nm [ 39 ]. The limit of detection for these indirect methods is commonly ≤2.0 mmol/L over the physiological range of 1.0 to 25 mmol/L [ 40 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and Quantitative Determination of Lactate Using Optical Spectroscopy—Towards a Noninvasive Tool for Early Recognition of Sepsis

Budidha

Mamouei

Baishya

et al. 2020

Sensors

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Uninterrupted monitoring of serum lactate levels is a prerequisite in the critical care of patients prone to sepsis, cardiogenic shock, cardiac arrest, or severe lung disease. Yet there exists no device to continuously measure blood lactate in clinical practice. Optical spectroscopy together with multivariate analysis is proposed as a viable noninvasive tool for estimation of lactate in blood. As an initial step towards this goal, we inspected the plausibility of predicting the concentration of sodium lactate (NaLac) from the UV/visible, near-infrared (NIR), and mid-infrared (MIR) spectra of 37 isotonic phosphate-buffered saline (PBS) samples containing NaLac ranging from 0 to 20 mmol/L. UV/visible (300–800 nm) and NIR (800–2600 nm) spectra of PBS samples were collected using the PerkinElmer Lambda 1050 dual-beam spectrophotometer, while MIR (4000–500 cm−1) spectra were collected using the Spectrum two FTIR spectrometer. Absorption bands in the spectra of all three regions were identified and functional groups were assigned. The concentration of lactate in samples was predicted using the Partial Least-Squares (PLS) regression analysis and leave-one-out cross-validation. The regression analysis showed a correlation coefficient (R2) of 0.926, 0.977, and 0.992 for UV/visible, NIR, and MIR spectra, respectively, between the predicted and reference samples. The RMSECV of UV/visible, NIR, and MIR spectra was 1.59, 0.89, and 0.49 mmol/L, respectively. The results indicate that optical spectroscopy together with multivariate models can achieve a superior technique in assessing lactate concentrations.

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Section: Discussionmentioning

confidence: 99%

Identification and Quantitative Determination of Lactate Using Optical Spectroscopy—Towards a Noninvasive Tool for Early Recognition of Sepsis

Budidha

Mamouei

Baishya

et al. 2020

Sensors

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“…The levels of lactate, released in the cell medium as a consequence of the glycolytic metabolism, was measured in both normoxic and hypoxic conditions by the spectrophotometric method described by Borshchevskaya et al [ 95 ], with some changes. To perform the assay, the cells were subjected to treatment with DR2B and DR2C extracts (500 μg/mL) for 24 h, both alone and in the presence of tBOOH (5 μM; 2 h exposure), then, the cell medium was collected for lactate levels determination.…”

Section: Methodsmentioning

confidence: 99%

A Polyphenol Rich Extract from Solanum melongena L. DR2 Peel Exhibits Antioxidant Properties and Anti-Herpes Simplex Virus Type 1 Activity In Vitro

et al. 2018

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DR2B and DR2C extracts, obtained by ethanolic maceration of peel from commercially and physiologically ripe aubergine berries, were studied for the antioxidative cytoprotective properties and anti-HSV-1 activity, in line with the evidence that several antioxidants can impair viral replication by maintaining reducing conditions in host cells. The antioxidative cytoprotective effects against tBOOH-induced damage were assessed in Caco2 cells, while antiviral activity was studied in Vero cells; polyphenolic fingerprints were characterized by integrated phytochemical methods. Results highlighted different compositions of the extracts, with chlorogenic acid and delphinidin-3-rutinoside as the major constituents; other peculiar phytochemicals were also identified. Both samples reduced reactive oxygen species (ROS) production and exhibited scavenging and chelating properties. DR2C partly counteracted the tBOOH-induced cytotoxicity, with a remarkable lowering of lactate metabolism under both normoxia and hypoxia; interestingly, it increased intracellular GSH levels. Furthermore, DR2C inhibited the HSV-1 replication when added for 24 h after viral adsorption, as also confirmed by the reduction of many viral proteins’ expression. Since DR2C was able to reduce NOX4 expression during HSV-1 infection, its antiviral activity may be correlated to its antioxidant properties. Although further studies are needed to better characterize DR2C activity, the results suggest this extract as a promising new anti-HSV-1 agent.

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“…Besides the measurement of weight loss, degradation was followed also by the determination of the main degradation product, lactic acid. The monomer forms a complex with iron(III) chloride hexahydrate which has an adsorption maximum at 390 nm in the UV-Vis spectrum (Borshchevskaya et al, 2016). 3 ml reagent solution (3 mg/ml) was added to 75 l degradation medium to form the complex.…”

Section: Degradation Studiesmentioning

confidence: 99%