Spectrophotometric Determination of Nifedipine and Nicardipine in their Pharmaceutical Preparations

Abstract

Genotoxic exposure to tobacco carcinogens resulting in DNA damage is an important mechanism of oral squamous cell carcinoma (OSCC) etiology. Nucleotide Excision Repair (NER) pathway removes bulky DNA adducts, generated from tobacco exposure thereby playing a major role in initiation of OSCC. In addition to mutations, epigenetic modifications have also been shown to target DNA repair genes thereby modulating oral tumor genesis. We therefore examined the role of epigenetic alterations modulating expression of three NER genes; XPC, XPB and XPD involved in removal of adducts caused by major classes of tobacco carcinogens and their contribution to OSCC Methylation status of NER genes was assessed using methylation specific PCR (MSP) in biopsies taken from 52 OSCC patients, their surrounding margins and 27 normal controls. The mRNA levels were determined using quantitative real time PCR (qRT-PCR) and Chromatin immuno precipitation (ChIP) analysis was performed to examine histone modifications in selected NER genes.We did not observe any significant difference in promoter methylation of NER genes between OSCC patients and controls. Increased XPB mRNA levels (p 0.04) and higher prevalence of H3 acetylation of XPB (p 0.04) gene were observed in OSCC patients as compared to controls.Our findings suggest the epigenetic modifications regulating the expression of XPB gene may be involved in OSCC etiology.

UV‐Light Photoelectrochemical Sensor Based on the Copper Tetraamino‐Phthalocyanine‐modified ITO Electrode for the Detection of Nifedipine in Drugs and Human Serum

“…Therefore, the determination of NP level in pharmaceutical formulations is indispensable for the control of industrial product quality and for ensuring the suitable dose for the patients. Modern instrumental analysis techniques such as high‐performance liquid chromatography (HPLC), gas chromatography, capillary gas chromatography, and spectrophotometry were established and applied to the determination of NP. Although HPLC and other combination technologies have high sensitivity and selectivity, these analytical methods involve costly and time‐consuming processes.…”

Metformin Loaded Carbopol Gel for lowering the Intra-Abdominal Visceral Fat

“…Briefly, cellophane membrane was stretched over the end of an open-ended glass tube and made water tight using a rubber band. The tube was immersed vertically in a 100 mL beaker containing 125 mL of phosphate buffer (pH 7.4) maintained in a thermostatically controlled shaker, 50 stroke/min maintained at 37.0 ± 1.0°C (WiseBath, WSB-45 DAIHAN Scientific Co., Ltd, Korea) [24]. METHCL gel formulation (1.0 g gel equivalent to 6mg METHCL) was placed into the glass tube.…”