Spectrophotometric determination of penicillins in pure and pharmaceutical formulations using Folin‐Ciocalteu reagent

Abstract

A new combination of time, temperature, and alkali is described for the spectrophotometric determination of amoxicillin and ampicillin using Folin-Ciocalteu reagent. The method is based on the development of blue-coloured product due to the reduction of tungstate and/or molybdate in Folin-Ciocalteu reagent by amoxicillin and ampicillin in alkaline medium. The chromogenic reaction has λ(max) at 720 and 740 nm with molar absorptivity 1.6295 × 10⁴ and 0.1085 × 10⁴ l mol⁻¹ cm⁻¹ in the Beer's Law range 2-10 µg mL⁻¹ and 10-70 µg mL⁻¹ for amoxicillin and ampicillin, respectively. The method is reproducible, quick, inexpensive, and particularly helpful in determining the drug content in commercial dosage forms.

Development and validation of RP-HPLC method for determination of amoxicillin residues and application to NICOMAC coating machine

“…2 AMO is official in British Pharmacopeia (BP), 1 European Pharmacopeia (EP) 3 and United States Pharmacopeia (USP) 4 , they include HPLC method for its determination. It is still a limited number of analytical methods that are reported for the determination of AMO including kinetics degradation, [5][6][7] spectrophotometric, [8][9][10][11][12][13] UHPLC UPLC and mass spectrometry, [14][15][16][17][18][19] thin layer chromatography (TLC), [20][21][22] capillary electrophoresis, [23][24][25][26] high performance liquid chromatography (HPLC), [27][28][29][30][31] in vitro dissolution studies, [32][33][34][35][36] amoxicillin residues in animal tissues using SPE-LC, 37 SPE-cation exchange, 38 in eggs using HPLC-FLD, 39 or HPLC-MS 40 and in commercial meat and milk samples 41 using HPLC-FLD. According to the best of our knowledge there is no validated method for the determination of amoxicillin residues and application to cleaning machine in pharmaceutical industries.…”

A rapid microtitre plate Folin-Ciocalteu method for the assessment of polyphenols

“…Originally this method was developed for the colorimetric determination of tyrosine, a phenolic nonessential amino acid [4]. Thereafter, this method has been used for the determination of several compounds including plant phenolics, drugs [5][6][7], vitamin C [8] and other constituents in a wide range of samples ranging from plant extracts to urine and bee products, in particular. The spectrophotometer-cuvette method is also frequently quoted.…”

Green Synthesis and Characterization of Magnetic Antibiotic Delivery System Using Linden Extract

“…Amoxicillin were analyzed in all supernatant specimens. The method described by Singh and Maheshwari (2010) was used for the determination of Amox (Singh and Maheshwari, 2010). In sum, 1.25 mL of 10% (w/v) Na2CO3 solution was added to 500 µL of the solution and tubes were left to react in a water bath at 98° C for 40 min.…”