Spectrophotometric determination of phosphorus as phosphate in organic compounds and materials of biological origin using a flow-injection manifold with a mixing chamber

Evaluation of serum electrolyte concentrations in Garole sheep of Sundarban region

“…Measurement of potassium (K+) and Sodium (Na+) was carried out by using flame photometry method (George et al, 1954) and Phosphorus (P3-) was determined by using spectrophotometry method (Burns et. al, 1992).…”

“…Phosphorus content was determined measuring the color intensity with the help of digital spectrophotometer (Model : Labtronics LT31) at 660 nm wave length as per method (Burns et al, 1992).…”

Assay of plant tissues for elemental content by flow injection analysis

“…In the designed two-line arrangement, the sample was injected in a HC1 carrier stream, merged with the vanadomolybdic acid reagent (added by confluence), and after appropriate reaction time, the product was measured at 400 nm. Burns et al (1992) determined P in organic compounds and materials of biological origin (including plants) by an entirely different approach. They developed a flow manifold with a mixing chamber where the P sample content was settled after measuring the turbidance at 560 nm of a colloidal insoluble blue dye salt solution formed by reaction of P with molybdate and crystal violet prepared in PVA.…”

Turbidimetric and Nephelometric Flow Analysis: Concepts and Applications

“…In order to investigate the feasibility of isothermal distillation in flow injection analysis, Zagatto et al [47] proposed a turbidimetric FI system with the Nessler reagent for the determination of total nitrogen in plant material. The merging zones approach was employed to add Nessler's reagent in a discrete way so as to avoid baseline drift, which happens when this reagent is added continuously, [48] and to diminish reagent consumption. The influence of surfactant, flow rates, alkalinity, ionic strength, collector stream pH, reagent concentration, and sample volume in ammonia distillation are discussed.…”

“…[49] Nevertheless, as an alternative to colorimetric procedures, different turbidimetric methodologies have been proposed (Table 2). Burns et al [48] developed a FI manifold with a mixing chamber for the determination of phosphate with molybdate and crystal violet. The insoluble blue dye salt is kept in colloidal solution with PVA and measured at 560 nm.…”