Spectrophotometric Determination of Polyphenols in Green Teas with 18-Molybdodiphosphate

Al-Shwaiyat, Mohammed K.E.A.; Денисенко, Т. А.; Miekh, Yuliia V.; Vishnikin, Andriy B.

doi:10.23939/chcht12.02.135

Cited by 11 publications

(7 citation statements)

References 25 publications

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“…The determination of the total phenolic content quantity was carried out with the Folin–Chokalteu reagent at 725 nm [ 72 , 73 ]. The content of phenylpropanoids was analyzed by direct spectrophotometry of solutions at 330 nm [ 74 ].…”

Section: Methodsmentioning

confidence: 99%

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

Aksenova

Nechaeva

Zubova

et al. 2023

Plants

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Plant tissue cultures are considered as potential producers of biologically active plant metabolites, which include various phenolic compounds that can be used to maintain human health. Moreover, in most cases, their accumulation is lower than in the original explants, which requires the search for factors and influences for the intensification of this process. In this case, it is very promising to use the precursors of their biosynthesis as potential “regulators” of the various metabolites’ formation. The purpose of our research was to study the effect of L-phenylalanine (PhA, 3 mM), trans-cinnamic acid (CA, 1 mM) and naringenin (NG, 0.5 mM), as components of various stages of phenolic metabolism, on accumulation of various phenolic compound classes, including phenylpropanoids, flavans and proanthocyanidins, as well as the content of malondialdehyde in in vitro callus culture of the tea plant (Camellia sinensis L.). According to the data obtained, the precursors’ influence did not lead to changes in the morphology and water content of the cultures. At the same time, an increase in the total content of phenolic compounds, as well as phenylpropanoids, flavans and proanthocyanidins, was noted in tea callus cultures. Effectiveness of precursor action depends on its characteristics and the exposure duration, and was more pronounced in the treatments with PhA. This compound can be considered as the most effective precursor regulating phenolic metabolism, contributing to a twofold increase in the total content of phenolic compounds, flavanes and proanthocyanidins, and a fourfold increase in phenylpropanoids in tea callus cultures.

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Section: Methodsmentioning

confidence: 99%

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

Aksenova

Nechaeva

Zubova

et al. 2023

Plants

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“…The total PC content was determined using the Folin–Chiocalteu reagent [ 79 , 80 ]. An amount of 75 µL of the FC reagent was added to 75 µL of the ethanol extract or ethanol (control), and 150 µL of a 20% Na 2 CO 3 solution and 1200 µL of distilled water were added after 3 min.…”

Section: Methodsmentioning

confidence: 99%

Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (Camellia sinensis L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols

et al. 2022

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Tea plants (Camellia sinensis L.) are phenol-accumulating crops that are widely used for public health. The healing effect of tea leaf products is due to the biosynthesis of such phenolic compounds (PCs) as flavans, which have P-vitamin capillary-strengthening activity. Due to their limited habitat and the value of their specialized metabolites of a phenolic nature, a promising approach is to establish in vitro cultures from them that retain the ability to form PCs, which is characteristic of ex vivo tea plants. The aim of this study was to investigate the effect of exogenic H2O2 (0.01 mM; 0.1 mM; 1 mM) on the growth, morphology, degree of stress response, and accumulation of various phenolic compounds in tea plant callus cultures of different ages (24 or 36 days) grown under different cultivation conditions (darkness or light). According to the results obtained, the H2O2 effect on tea callus cultures of different ages did not cause changes in their morphophysiological characteristics, both after 2 h of exposure (rapid response of callus culture, RRCC) and after 48 h (delayed response of callus culture, DRCC). The determination of the malondialdehyde (MDA) content, which serves as an indicator of changes in the level of lipid peroxidation (LPO) and the presence of stress responses in plant cells, indicated either its maintenance at the control level, a decrease, or an increase. All these effects depended on the growth conditions of the tea callus cultures (darkness or light), their age, the duration of exposure (rapid or delayed response), and the H2O2 concentration. Similar trends were noted for the total content of PCs as well as the amount of flavans, proanthocyanidins (soluble and insoluble forms), and lignin. The plant cell responses reflected changes in its adaptation programs, when specialized metabolites act as a target for the action of H2O2, thereby contributing to an increase in their resistance.

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“…In addition to the two chromogenic reagents mentioned above, there are also new chromogenic reagents for the determination of total tea polyphenols by visible spectrophotometry. Al-Shwaiyat et al used 18-MPC (18-molybdodiphosphate heteropoly complex) as a chromogenic reagent to determine the total tea polyphenols in green tea, showing a higher selectivity than Folin-Ciocalteu reagent [34].…”

Section: Spectrophotometrymentioning

confidence: 99%

Recent Advances in Analytical Methods for Determination of Polyphenols in Tea: A Comprehensive Review

Sun

Jiang

Kong

et al. 2022

Foods

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Polyphenols, the most abundant components in tea, determine the quality and health function of tea. The analysis of polyphenols in tea is a topic of increasing interest. However, the complexity of the tea matrix, the wide variety of teas, and the difference in determination purposes puts forward higher requirements for the detection of tea polyphenols. Many efforts have been made to provide a highly sensitive and selective analytical method for the determination and characterization of tea polyphenols. In order to provide new insight for the further development of polyphenols in tea, in the present review we summarize the recent literature for the detection of tea polyphenols from the perspectives of determining total polyphenols and individual polyphenols in tea. There are a variety of methods for the analysis of total tea polyphenols, which range from the traditional titration method, to the widely used spectrophotometry based on the color reaction of Folin–Ciocalteu, and then to the current electrochemical sensor for rapid on-site detection. Additionally, the application of improved liquid chromatography (LC) and high-resolution mass spectrometry (HRMS) were emphasized for the simultaneous determination of multiple polyphenols and the identification of novel polyphenols. Finally, a brief outline of future development trends are discussed.

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Spectrophotometric Determination of Polyphenols in Green Teas with 18-Molybdodiphosphate

Cited by 11 publications

References 25 publications

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

Effects of Hydrogen Peroxide on In Vitro Cultures of Tea (Camellia sinensis L.) Grown in the Dark and in the Light: Morphology, Content of Malondialdehyde, and Accumulation of Various Polyphenols

Recent Advances in Analytical Methods for Determination of Polyphenols in Tea: A Comprehensive Review

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