Spectrophotometric determination of sulfide based on peroxidase inhibition by detection of purpurogallin formation

Spectrophotometric determination of hydrogen sulfide in environmental samples using sodium 1,2-naphthoquinone-4-sulfonate and response surface methodology

“…We compared the results of some of the spectrophotometric methods (Yang et al 2009;Ghadiri et al 2013;Peng et al 2011;Safavi and Ramezani 1997;Zhang et al 2015;Bae et al 2013;Cmelik et al 2010;Evans 1967;Silva et al 2001) for determination of hydrogen sulfide (Table 6). The linear range of the method presented here is satisfactory and relatively wide.…”

Inhibition of Horseradish Peroxidase (HRP) by a Nonhydrophobic Component of Urine: A Caution for Immunoassays

“…They may involve endogenously generated cyano, azide, or other amine compounds capable of interacting with the active site of peroxidase [2,4], hydroxamic acids, hydrazides, and amides that alter spectral behavior of peroxidase as well as its kinetic characteristics [16], or homogentisic acid which inhibits catalysis at high concentrations [17]. They might also be thio-compounds such as sulfide [18], thioureas [19] or thiouracils [11] that seem to inhibit via interference with a thio group in the vicinity of the prosthetic group of the enzyme; L-cystine or cysteine reach uM concentrations in normal urine [6,7] and can also inhibit HRP [20], possibly through a similar mechanism. The water soluble cations Cd +2 are all present in at least trace quantities in urine and are known inhibitors of HRP through either catalytic interference or enzyme conformational change [20].…”

Selective determination of sulphide based on photoluminescence quenching of MPA-capped CdTe nanocrystals by exploiting a gas-diffusion multi-pumping flow method

“…24 Therefore, considering the harmful effects of H 2 S in human health, environment and industrial production quality, a wide range of analytical methodologies have been developed in order to monitor the level of sulphide ions in different types of samples. These analytical methodologies involve spectrophotometry, [25][26][27][28] uorometry, 20,[29][30][31][32][33][34] electrochemical methods, [35][36][37][38] HPLC, 39,40 gas chromatography, 41,42 ion chromatography 43 and capillary electrophoresis. 44 In addition, distinct continuous ow methodologies resorting to a variety of detection techniques were also proposed, including uorometric, 45 spectrophotometric, 46 chemiluminometric 22 and electrochemical 47 detection.…”