Spectrophotometric Estimation of Drugs Using N-Bromo Succinamide and Indigo Caramine Couple

Abstract

Simple, specific, accurate and precise UV-visible spectrophotometric methods have been developed for the estimation of five drugs viz., Dobutamine hydrochloride (DOB), Domperidone(DOM), Duloxetine hydrochloride(DUL), Phenylephrine(PHE) and Trimetazidine(TRM). These methods involve the addition of a known excess of NBS to the drugs in acid medium followed by estimation of residual NBS by reacting with a fixed amount of Indigo Caramine and measuring the absorbance at 520nm. Beer's law is obeyed in the concentration range of 0.6-4.2, 0.4-2.8, 0.3-2.1, 0.8-5.6 and 0.4-2.8 µg mL -1 for DOB, DOM, DUL, PHE and TRM respectively. Different variables affecting the reaction were studied and optimized. The proposed methods were applied successfully to the determination of the examined drugs in pure and pharmaceutical dosage forms with good accuracy and precision. The proposed methods were found to be successful for the estimation of these drugs in bulk and their formulations. The results of analysis have been validated statistically for linearity, accuracy, precision, LOD and LOQ.

Structure investigation of solid ion pairs of three imidazole drugs-Rose Bengal using different spectroscopic techniques and Their Biological Activities

“…Butoconazole nitrate, [(±) -1-[4-(p-Chlorophenyl)-2-[2,6-dichlorophenyl) thiol] butyl]-1H-imidazole mononitrate] (III), is an imidazole antifungal; which is primarily used for the local treatment of vulvovaginal candidiasis (infections caused by Candida) [8][9]. Due to the vital importance of these azole antifungals several methods have been reported for the determination of them either in pure forms, dosage forms, or biological fluids like chromatography [10][11][12][13][14][15][16], spectrophotometric [17][18][19][20], electrometric [21][22]. Structure investigation is an important part of drug development due to the need to identify the low level of impurities and degradation of drugs.…”

Spectrophotometric and Spectrofluorimetric Determination of Terazosin in Tablets by Eosin Y

“…Therefore it was indicated for the treatment of hypertension, or to improve urination in men with benign prostatic hyperplasia (1,2). Analytical literature review shows a number of methods to determine trerazosin in pharmaceutical preparations and biological fluids such as: reversed phase high performance liquid chromatography, HPLC (3)(4)(5), high performance thin layer chromatography, HPTLC (4,6), Electrochemical (7)(8)(9)(10)(11) spectrophotometric methods (12)(13)(14)(15)(16)(17)(18), and spectroflurimetric methods (19)(20)(21) The aim from this work is the development of two methods That are simple, accurate, sensitive and fast utilizing spectrophotometric and spectrofluorimetric and applying to pharmaceutical preparations using the eosineY dye. The present methods do not need to control the temperature or extraction process.…”

Simple spectrophotometric method for estimation of drugs using chloraminet and indigo caramine dye couple

“…It acts as a stimulator for alpha receptors in certain areas of the body. It is used locally, as decongestant, for non-specific and allergic conjunctivitis, sinusitis and nasopharyngitis [10][11][12][13][14][15]. Chemically Tramadol is (2-(dimethylaminomethyl)-1-(3methoxyphenyl) cyclohexanol) ( Figure 1C).…”