Spectrophotometric investigation of DNA in the ultraviolet

Basu, S.; Dasgupta, Nirjhar

doi:10.1016/0005-2787(67)90057-3

Cited by 13 publications

(7 citation statements)

References 14 publications

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“…The nature of increase in Y/X ratio with v-dose is found to be DNA concentration dependent and does show more sensitivity of Y-peak to v-irradiation. Similar increase is also reported for Y/X ratio on thermal denaturation of DNA (Falk, 1964;Basu and Dasgupta, 1967).…”

Section: Behaviour Of Y/x Ratio On V-irradiationsupporting

confidence: 88%

“…It will be wrong to assume that Y-peak is an entirely solvent governed phenomenon. Hyperchromicity change during thermal transition at Y-peak is 14% more than that at X-peak (Basu and Dasgupta, 1967). Y-peak follows Bear Lambert Law for the concentration range tried.…”

Section: Irradiation Effect On Y-peakmentioning

confidence: 82%

“…Another parameter considered here is to compare the ratio Y/X of the two peak's (Voet et al, 1963;Falk, 1964;Basu, 1967;Basu and Dasgupta, 1967). (Wheeler, 1978;Wheeler and Raft, 1973;Raft et al, 1968).…”

Section: Ratio: Y/xmentioning

confidence: 99%

“…Some investigations (Basu and Dasgupta, 1967), in particular, reflect the behaviour of Y-peak when DNA is subjected to thermal denaturation in relation to more prominent, 258 nm peak (X-peak) and it was found that hyperchromicity change at Y-peak is 14% more than at X-peak. Relative sensitivity of Y-peak to ionic change (Basu and Dasgupta, 1967) and thermal transition compared to X-peak requires further explanation. Moreover, the presence of Y-peak, which is sharper than X-peak, speaks of strong ~-~* transition in far UV region.…”

Section: Introductionmentioning

confidence: 99%

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Dependence of?-irradiated Y-peak and melting of DNA on concentration and ionic environment

Attri

Mookerjee

1981

Radiat Environ Biophys

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Y-peak is found to be a function of ionic strength and concentrations of DNA. The Y-peak reveals close dynamic interaction between DNA and solvent system. Electronic transitions responsible for Y-peak are not the same transitions that are responsible for X-peak. Y-peak's electronic transitions are indicative of charge transfer complex formation between DNA and solvent system. gamma-Irradiation induces hyperchromicity due to strand separation at lower doses. A-T base pairs are first to undergo coiled state as shown by delta Tm spread. Strand chopping and saturation of double bonds of the exposed bases by free radicals (H degrees and OH degrees) give rise to hypochromic regions at X-peak. Rise in ionic strength and the concentration of DNA has protective effect against gamma-damage. Y-peak is found to be a function of solvent, whereas, X-peak is independent of solvent nature.

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Section: Behaviour Of Y/x Ratio On V-irradiationsupporting

confidence: 88%

Section: Irradiation Effect On Y-peakmentioning

confidence: 82%

Section: Ratio: Y/xmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Dependence of?-irradiated Y-peak and melting of DNA on concentration and ionic environment

Attri

Mookerjee

1981

Radiat Environ Biophys

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“…To determine the structural alteration of DNA during the reaction with fructose, UV spectroscopy and fluorescence study were employed. Ultraviolet spectroscopy is often used to determine the conformational changes of chromophores occurring in DNA 26 – 28 . 5, 10, and 15 days after glycation, the trend of the kinetic changes at 260 nm continued to increase.…”

Section: Discussionmentioning

confidence: 99%

Rosmarinic acid inhibits DNA glycation and modulates the expression of Akt1 and Akt3 partially in the hippocampus of diabetic rats

Alrubaye

Motovali-Bashi

Miroliaei

2021

Sci Rep

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Non-enzymatic glycation of DNA and the associated effects are among pathogenic factors in diabetes mellitus. Natural polyphenols have anti-diabetic activity. Herein, the protective role of one of the phytochemicals, rosmarinic acid (RA), was evaluated in glycation (with fructose) of human DNA and expression of Akt genes in the hippocampus of diabetic rats. In-vitro studies using fluorescence, agarose gel electrophoresis, fluorescence microscopy, and thermal denaturation analyses revealed that glycation causes DNA damage and that RA inhibits it. In-vivo studies were performed by induction of diabetes in rats using streptozotocin. The diabetic rats were given RA daily through gavage feeding. The expression of Akt genes (inhibitors of apoptosis) in the hippocampus was evaluated using RT-qPCR. In diabetic rats, Akt1 and Akt3 were significantly down-regulated compared to the control group. Treating the diabetic rats with RA returned the expression of Akt1 and Akt3 relatively to the normal condition. Past studies have shown that diabetes induces apoptosis in the hippocampal neurons. Given that glycation changes the genes expression and causes cell death, apoptosis of the hippocampal neurons can be due to the glycation of DNA. The results also suggest that RA has reliable potency against the gross modification of DNA under hyperglycemic conditions.

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Structure of Viral Nucleic Acids in Situ

Tikchonenko

1975

Structure and Assembly

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Spectrophotometric investigation of DNA in the ultraviolet

Cited by 13 publications

References 14 publications

Dependence of?-irradiated Y-peak and melting of DNA on concentration and ionic environment

Dependence of?-irradiated Y-peak and melting of DNA on concentration and ionic environment

Rosmarinic acid inhibits DNA glycation and modulates the expression of Akt1 and Akt3 partially in the hippocampus of diabetic rats

Structure of Viral Nucleic Acids in Situ

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