Structure of Viral Nucleic Acids in Situ

Tikchonenko, T.I.

doi:10.1007/978-1-4684-2709-7_1

Cited by 7 publications

(10 citation statements)

References 293 publications

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“…These micrographs suggest that the reported distribution of T2 toroid sizes may actually be a result of partial toroid decondensation after their expulsion from phage heads. If we consider bacteriophage toroids as having a circular cross section, an approximation supported by previous investigations of toroidal DNA condensates (Arscott et al, 1990), then a toroid volume calculated from the mean value of dimensions reported for T2 toroids is only 50% of the expected volume for a fully condensed T2 genome (Tikchonenko, 1975), whereas the volume of a T2 toroid that lies at the upper end of the reported dimensions is large enough to account for 96% of the expected volume of a fully condensed T2 genome. Thus we believe that the most probable dimensions for a toroid inside a T2 head are those of the largest volume toroid observed by Klimenko et al (1967), namely 1300 A in outside diameter with a 500 A diameter hole.…”

Section: Introductionmentioning

confidence: 74%

“…Some investigators (Tikchonenko, 1975;Lepault et al, 1987) have expressed the opinion that although they may be related to the organization of DNA within intact phage heads, the presence of toroids in phage lysates is most likely the result of DNA rearrangement after its expulsion from phage heads. However, this presumed rearrangement has never been proven.…”

Section: Introductionmentioning

confidence: 99%

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Double-stranded DNA organization in bacteriophage heads: an alternative toroid-based model

Hud¹

1995

Biophysical Journal

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Studies of the organization of double-stranded DNA within bacteriophage heads during the past four decades have produced a wealth of data. However, despite the presentation of numerous models, the true organization of DNA within phage heads remains unresolved. The observations of toroidal DNA structures in electron micrographs of phage lysates have long been cited as support for the organization of DNA in a spool-like fashion. This particular model, like all other models, has not been found to be consistent will all available data. Recently we proposed that DNA within toroidal condensates produced in vitro is organized in a manner significantly different from that suggested by the spool model. This new toroid model has allowed the development of an alternative model for DNA organization within bacteriophage heads that is consistent with a wide range of biophysical data. Here we propose that bacteriophage DNA is packaged in a toroid that is folded into a highly compact structure.

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Section: Introductionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Double-stranded DNA organization in bacteriophage heads: an alternative toroid-based model

Hud¹

1995

Biophysical Journal

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“…Using IR-and Raman spectroscophy [l-3] and X-ray diffraction [4,5] it has been established that the overwhelming part of DNA within bacteriophages has the B-form. As yet, however, it remains obscure, whether all intraphage DNA has the double-stranded B-form or whether some part of the DNA bases is not involved in complementary interactions [5].…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

“…As yet, however, it remains obscure, whether all intraphage DNA has the double-stranded B-form or whether some part of the DNA bases is not involved in complementary interactions [5]. Here, bromoacetaldehyde (BAA) was used to study the secondary structure of DNA in h phage.…”

Section: Introduction 2 Materials and Methodsmentioning

confidence: 99%

Fluorescence study of secondary structure of DNA within bacteriophage λ

Shurdov¹,

Kiyanov²

1983

FEBS Letters

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Bromoacetaldehyde (BAA) was used to study the secondary structure of DNA in A-phage particles. It was determined that about 1% of the adenines in the intraphage A-DNA reacts readily with BAA, thus, they are placed in DNA sites with disturbed complementary interactions. These adenines are close to the tryptophan residues of the phage protein. Fluorescence emission of EA in the intraphage DNA is dramatically quenched. This, apparently, indicates the interaction between CA and Trp-and/or Tyrand/or Met-residues of phage protein.

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“…One such approach is the study of RNA-protein crosslinking in virus particles. As intravirus TMV RNA is highly resistant to chemical modification (for a review see Tikchonenko, 1975), UV-irradiation seems to be the obvious choice. Previously, in the literature there were some indications of possible UV-induced RNA-protein crosslinking in TMV but no definite conclusions could be drawn (Goddard et al, 1966;Streeter and Gordon, 1968).…”

Section: Introductionmentioning

confidence: 99%

Rna—protein Crosslink and Rna Chain Break Formation on Uv‐irradiation of Tobacco Mosaic Virus

Добров

Arbieva

Khromov

et al. 1986

Photochem & Photobiology

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Abstract— The ability of UV‐irradiation (254 nm) to induce formation of RNA‐protein crosslinks in tobacco mosaic virus (TMV) particles have been studied by Cs2SO4 density gradient centrifugation, analytical centrifugation, nitrocellulose filter binding and two‐dimensional peptide mapping. RNA‐protein crosslinks were found to be formed on UV‐irradiation of TMV, but the parallel process of UV‐induced RNA chain breakage complicated their quantitation. Using speciall devised equations, the quantum yield of RNA‐protein crosslink formation was found to be 0.65 × 10−5 and that of RNA chain break formation 0.95 × 10−5.

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Structure of Viral Nucleic Acids in Situ

Cited by 7 publications

References 293 publications

Double-stranded DNA organization in bacteriophage heads: an alternative toroid-based model

Double-stranded DNA organization in bacteriophage heads: an alternative toroid-based model

Fluorescence study of secondary structure of DNA within bacteriophage λ

Rna—protein Crosslink and Rna Chain Break Formation on Uv‐irradiation of Tobacco Mosaic Virus

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