Spectrophotometric method for the determination of ethanol in beverages and beer samples using cerium (IV) as reagent

In this work different ways of optimally producing bioethanol at various pH with thermotolerant yeasts and their cocultures using a non-human edible starchy food as feedstock was examined. African wild cocoyam, Xanthosoma roseum, sourced from abandoned farmlands in Obukpa, Nsukka, Nigeria was used as the substrate, while strains of Kluyveromyces marxianus and Pichia stipitis were used to ferment them. First the tubers were gelatinized by boiling under pressure above 100oC before hydrolysis with concentrated H2SO4. The hydrolysates were then fermented at 35oC with the thermotolerant yeasts for five days at different pH. Results obtained showed that gelatinized sample of the substrate gave optimum glucose yield when hydrolysed with 1M H2SO4 for 60 minutes. Kluyveromyces marxianus produced more ethanol than Pichia stipitis at all the four fermentation pH values tested. However, optimum ethanol production was obtained when the two yeast strains were used as coculture at pH 4.5. The peak time for ethanol production was 96 hours for the individual yeast cultures while that of their coculture was 72 hours. The results of the study indicated that wild cocoyam is an excellent feedstock for bioethanol production with many advantages including being non-edible, thereby eliminating concerns for food security, and containing high amount of carbohydrate. The study also revealed that fermenting sugar hydrolysates with a coculture of microorganisms during bioethanol production is a more efficient process than using individual cultures.

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“…The method described by Lau and Luk [14] was used. One (1) mL of the sample filtrate was taken into a test tube.…”

Section: Determination Of Sugar Concentrationmentioning

confidence: 99%

“…The pure isolates obtained afterwards were stored on agar slants at 4 o C for subsequent use. The yeasts isolates were then characterized and identified on the basis of their colony morphology and biochemical characteristics using Mycology Atlas [15] as guide.…”

Section: Thermotolerant Yeast Isolation and Identificationmentioning

confidence: 99%

Studies on Bioethanol Production with Thermo Tolerant Yeast Isolates and their Co-Cultures using African Wild Cocoyam as Feedstock

Chukwudi

OkeChukwu

Ifeanyi

et al. 2021

AJB2T

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“…Residual glucose in fermented culture was determined with glucose kits according to by method of Young and Friedman (2001). Bioethanol in fermented culture was determined colormetrically according to the method of Lau and Luk (1994). Yield of bioethanol and productivity were calculated according to Gamal et al (1991) using the following equations: Yield (%) = Bioethanol produced (gl -1 ) x100…”

Section: Chemical Determinationsmentioning

confidence: 99%

Bioconversion of Various Agro-Industrial Byproducts Into Bioethanol by Two Local Yeast Strains Using Static Culture Technique

Tayeb

Abdelhady

Salem

et al. 2016

Egyptian Journal of Agricultural Research

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ome agro-industrial byproducts [sugar cane molasses (SM), corncobs waste (CW), sugar cane bagasse (SB), sawdust (SD), sugar beet pulp (SBP) and fruit juice waste (FJW)] were used as substrates for bioethanol production by Clavispora lusitaniae Gr45 and Saccharomyces cerevisiae B1 after 3 days incubation at 30°C in static batch culture. Different treatments of these materials as a sole carbon source were applied with or without nitrogen sources (yeast extract or ammonium sulfate). The highest bioethanol concentration when SM is used (13.05 gl -1 ) was obtained by Sacch. cerevisiae at the treatments: [yeast fermentation medium (YFM) -glucose + 4 % SM], (yeast extract + 6 % SM) and (10% SM), which represent about 10.6 % increase comparing to YFM medium (control). The addition of acid CW hydrolyzate as the sole carbon source to YFM medium was the best of the CW treatments by Cl. lusitaniae Gr45 which recorded the highest bioethanol concentration, productivity, yield and conversion coefficient being, 13.32 gl -1 , 0.18 gl -1 h -1 , 26.64 % and 26.65 %; respectively. These figures were increased to 15.8 gl -1 , 0.22 gl -1 h -1 , 31.6 % and 35.9 %, respectively, on YFM medium containing acid SB hydrolyzate as the sole carbon source. All acid SD hydrolyzate treatments increased bioethanol production by Cl. lusitaniae Gr45 and decreased it by Sacch. cerevisiae B1. The highest bioethanol production obtained on SD was on SD hydrolyzate containing yeast extract followed by SD hydrolyzate only and SD hydrolyzate containing ammonium sulfate being 16.5, 13.76 and 13.54 gl -1 ; respectively. Using SBP as sole carbon source added to YFM medium decreased significantly the growth and bioethanol production by both strains. Generally, FJW was the best carbon source in YFM medium which increased bioethanol production to about 68 % and 38 % by Sacch. cerevisiae B1 and Cl. lusitaniae Gr45; respectively using static culture technique.

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“…Baker's yeast was inoculated at 2 % w/v in either flasks or bioreactor cultivations. Flasks were incubated in an anaerobic incubator (Hirayama Manufacturing Corp., Tokyo, Japan) at 30°C for 4 d. The overall bioethanol concentration was determined colormetrically according to the method of Lau & Luk (1994).…”

Section: Bioethanol Production From Biotreated Agro-industrial Wastesmentioning

confidence: 99%

Fungal Biotreatment of Agro-Industrial Wastes for the Production of Bioethanol in Bioreactor

2014

Egypt. J. Microbiol.

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IOETHANOL production from lignocellulosic feedstocks is considered a promising strategy to increase global production of biofuels without impacting food supplies. This work aimed to evaluate bioethanol production by baker's yeast using a medium containing the hydrolysate of fungal biotreatment of five different lignocellulosic feedstocks with some amendments. The pretreatment of lignocellulosic feedstocks using 5 % w/v NaOH, 1 % v/v H 2 SO 4 and sodium hypochlorite: H 2 O 2 (10:1) prior to fungal biotreatment was studied. For bioethanol production, batch, fed-batch (two strategies) and continuous cultivations of baker's yeast on the fungal biotreated rice straw hydrolysate was evaluated in bioreactor. In batch and pulsed fed-batch cultivations, the highest bioethanol concentration, conversion coefficient, bioethanol yield and productivity were (0.41 % v/v, 36.9 % v/w, 36.9 % v/w and 0.114 ml/l/h, respectively), while in fed-batch cultivation with continuous feeding these parameters were (0.45 % v/v, 40 % v/w, 40.5 v/w % and 0.015 ml/l/h, respectively). The highest bioethanol concentration (0.52 % v/v) was obtained in continuous culture at dilution rate of 0.03 h-1 , while conversion coefficient, yield and productivity were 31.2 % v/w, 31.4 % v/w and 0.022 ml/l/h, respectively.

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Spectrophotometric method for the determination of ethanol in beverages and beer samples using cerium (IV) as reagent

Cited by 7 publications

References 5 publications

Studies on Bioethanol Production with Thermo Tolerant Yeast Isolates and their Co-Cultures using African Wild Cocoyam as Feedstock

Studies on Bioethanol Production with Thermo Tolerant Yeast Isolates and their Co-Cultures using African Wild Cocoyam as Feedstock

Bioconversion of Various Agro-Industrial Byproducts Into Bioethanol by Two Local Yeast Strains Using Static Culture Technique

Fungal Biotreatment of Agro-Industrial Wastes for the Production of Bioethanol in Bioreactor

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