Spectrophotometric nanomolar determination of glucose by using C-dots/$$\hbox {Fe}_{3}\hbox {O}_{4}$$ magnetic nanozyme

Abstract

C-dots/Fe 3 O 4 magnetic nanocomposite was known as peroxidase-like nanozyme. In this work, C-dots/Fe 3 O 4 magnetic nanocomposite was used to determine glucose in the presence of glucose oxidase (GOx). The color change of 3,3 ,5,5-tetramethylbenzidine (TMB) was used as an indication of glucose in a colorimetric assay. The nanozymatic activity of C-dots/Fe 3 O 4 magnetic nanocomposite to detect glucose was depended on TMB and C-dots/Fe 3 O 4 nanozyme concentration and pH. The linear range and detection limit to determine glucose using C-dots/Fe 3 O 4 magnetic nanocomposite were obtained as 5 × 10 −8 to 1 × 10 −3 M and 50 nM, respectively. Also, the sensing system shows high selectivity towards the detection of glucose in the presence of fructose, lactose and maltose as interferences. The results indicate that this assay is simple, cheap, and highly sensitive and selective for glucose determination.

Recent advancements in noninvasive glucose monitoring and closed-loop management systems for diabetes

“…The detection of glucose is realized by the change in color and fluorescence or chemiluminescence of oxidative products of H 2 O 2 . In recent years, many nanocomposites like C-dots/V 2 O 5 53 and C-dots/Fe 3 O 4 54 have been used for glucose detection. Moreover, noble metals and their composites like Pt-PIL-MWCNTs (Fig.…”