Spectrophotometry and colorimetry profiling of pure phenol red and cell culture medium on <scp>pH</scp> variation

Raffay, Razemy; Husin, Norfaiqah; Omar, Ahmad

doi:10.1111/cote.12626

Cited by 6 publications

(3 citation statements)

References 26 publications

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“…Phenol red is ideal for this application not only because of its high sensitivity to pH changes, but also because its yellow–red range is more evident than those seen on pH test strips. 25 As anticipated, the addition of 2 µL urine from PSS mice was sufficient to shift the color of 200 µL phenol red solution (0.1 mg/mL, pH 7.4) to yellow ( Figure 4C ). Conversely, non-PSS mice urine modestly shifted the phenol red color to orange–red ( Figure 4C ).…”

Section: Resultssupporting

confidence: 67%

Urine-based Detection of Congenital Portosystemic Shunt in C57BL/6 Mice

et al. 2023

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Sporadic occurrence of congenital portosystemic shunt (PSS) at a rate of ∼1 out of 10 among C57BL/6 mice, which are widely used in biomedical research, results in aberrancies in serologic, metabolic, and physiologic parameters. Therefore, mice with PSS should be identified as outliers in research. Accordingly, we sought methods to, reliably and efficiently, identify PSS mice. Serum total bile acids ≥ 40 µM is a bona fide biomarker of PSS in mice but utility of this biomarker is limited by its cost and invasiveness, particularly if large numbers of mice are to be screened. This led us to investigate if assay of urine might serve as a simple, inexpensive, non-invasive means of PSS diagnosis. Metabolome profiling uncovered that Krebs cycle intermediates, i.e., citrate, α-ketoglutarate, and fumarate, were strikingly and distinctly elevated in the urine of PSS mice. We leveraged the iron-chelating and pH-lowering properties of such metabolites as the basis for three urine-based PSS screening tests: urinary iron-chelation assay, pH strip test, and phenol red assay. Our findings demonstrate the feasibility of using these colorimetric assays, whereby their readout can be assessed by direct observation, to diagnose PSS in an inexpensive, rapid, and non-invasive manner. Application of our urinary PSS screening protocols can aid biomedical research by enabling stratification of PSS mice, which, at present, likely confound numerous ongoing studies.

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Section: Resultssupporting

confidence: 67%

Urine-based Detection of Congenital Portosystemic Shunt in C57BL/6 Mice

et al. 2023

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“…Cells were inspected employing an inverted optical microscope DMiL (Leica) equipped with a 3.1 Mpixel camera Optikam Pro 3 (Optika). Clarity and pH of the cell culture medium were also assessed visually 47 and a colorimetric cell viability MTT assay 48 was performed.…”

Section: Cytotoxicity Controlmentioning

confidence: 99%

Antiviral and Antibacterial Efficacy of Nanocomposite Amorphous Carbon Films with Copper Nanoparticles

Bakhet,

Tamulevičienė,

Vasiliauskas

et al. 2023

Preprint

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Copper compound-rich films and coatings are effective against widespread viruses and bacteria. Even though the killing mechanisms are still debated it is agreed that the metal ion, nanoparticle release, and surface effects are of paramount importance in the antiviral and antibacterial efficacy of the surfaces. In this work we have investigated the behaviour of the reactive magnetron sputtered nanocomposite diamond-like carbon thin films with copper nanoparticles (DLC:Cu). The films were etched employing oxygen plasma and/or exposed to ultra-pure water aiming to investigate the differences of the Cu release in the medium and changes in film morphology. Pristine films were more effective in the Cu release reaching up to 1.3 mg/L/cm2concentration. Plasma processing resulted in the oxidation of the films which released less Cu but after exposure to water, their average roughness increased more, up to 5.5 nm. Pristine and O2plasma processed DLC:Cu films were effective against both model coronavirus and herpesvirus after 1-hour contact time and reached virus reduction up to 2.23 and 1.63 log10, respectively. Pristine DLC:Cu films were more effective than plasma-processed ones against herpesvirus, while less expressed difference was found for coronavirus. A bactericidal study confirmed that pristine DLC:Cu films were effective against gram-negativeE. coliand gram-positiveE. faecalisbacteria. After 3 hours 100% antibacterial efficiency (ABE) was obtained forE. coliand 99.83% forE. faecalis. After 8 hours and longer exposures, 100% ABE was reached. The half-life inactivation of viruses was 8.10 – 11.08 minutes and forE. faecalis19.5 – 30.2 minutes.

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“…Phenol red (C 19 H 14 O 5 S; CAS: 143-74-8; molar mass 354.38 g mol –1 ; Figure ) is a conventional pH indicator, which can be used to determine the pH of seawater and other water qualities in an ecological environment . Scientists also often use phenol red as a medium indicator, − which can not only monitor the pH value of cell growth but also distinguish normal cells from cancer cells by using the color change of phenol red in the medium . Moreover, phenol red is a weak estrogen that can affect hormone response, such as regulating prolactin secretion in vitro and promoting cell proliferation in different cell lines .…”

Section: Introductionmentioning

confidence: 99%

Solubility Determination and Analysis of Phenol Red in Monosolvent and Binary Mixed Solvent at T = 278.15–323.15 K

Cheng,

Zhang,

Yang

et al. 2023

J. Chem. Eng. Data

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The solubility and thermodynamic characteristics of phenol red were determined by the static equilibrium method combined with HPLC. The equilibrium solubility of phenol red in 12 monosolvents (methanol, ethanol, npropanol, isopropanol, n-butanol, isobutanol, acetonitrile, methyl acetate, ethyl acetate, DMF, water, and acetone) was measured at a temperature range of 278.15−323.15 K and a constant pressure of 101.1 kPa. The results showed that in the experimental temperature range, the solubility of phenol red is positively correlated with the temperature and reached the maximum and minimum values in DMF and isobutanol. Ethanol, acetonitrile, and water were randomly selected to form three groups of binary mixed solvents, the monosolvent with high solubility is used as the cosolvent in the binary mixed solvent, and the solubility data were measured under the same experimental conditions. Interestingly, in all binary mixed solvents, the solubility data of phenol red showed a trend of first increasing and then decreasing with the increase of the proportion of cosolvent. The Buchowski−Ksiazaczak λh model and Apelblat model were used to fit the solubility data of phenol red in monosolvent, and the average root mean square deviation (RMSD) values are 3.8775 × 10 −2 and 4.6592 × 10 −3 . The Jouyban−Acree model, CNIBS/R-K model, and Sun model were used to fit the solubility data of phenol red in binary mixed solvent, and the average RMSD values were 9.4265 × 10 −6 , 5.1215 × 10 −6 , and 9.4265 × 10 −6 . Furthermore, the solvation of phenol red was investigated by the KAT-LSER model. The results showed that the acidity of the hydrogen bond had a significant effect on the solubility, and the contribution rate was 35.5%.

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Spectrophotometry and colorimetry profiling of pure phenol red and cell culture medium on pH variation

Cited by 6 publications

References 26 publications

Urine-based Detection of Congenital Portosystemic Shunt in C57BL/6 Mice

Urine-based Detection of Congenital Portosystemic Shunt in C57BL/6 Mice

Antiviral and Antibacterial Efficacy of Nanocomposite Amorphous Carbon Films with Copper Nanoparticles

Solubility Determination and Analysis of Phenol Red in Monosolvent and Binary Mixed Solvent at T = 278.15–323.15 K

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