Spectroscopic assessment of oxidative damage in biomolecules and tissues

Caliskan, Sükrü; Oldenhof, Harriëtte; Brogna, Raffaele; Rashidfarokhi, Bita; Sieme, Harald; Wolkers, Willem F.

doi:10.1016/j.saa.2020.119003

Cited by 9 publications

(5 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We previously used NBT staining to detect oxidative damage in ovarian cortex tissues stored in liquid 13 . In liquid, NBT reduction in these tissues was detected within several hours, and could only be effectively stopped at low subzero temperatures.…”

Section: Discussionmentioning

confidence: 99%

“…Nitroblue tetrazolium (NBT) was used to visualize and quantify accumulation of reactive oxygen species (ROS) in tissues, during dried storage as previously described 13 . NBT forms formazan upon reacting with ROS/superoxide which is evident as a blue/purple precipitate.…”

Section: Methodsmentioning

confidence: 99%

“…PCA was performed in different spectral regions, including the 3000–2800 and 1800–900 cm −1 regions. Prior to PCA, these spectral regions were vector normalized as previously described 13 . PCA was carried out using standard functionality of the statistical programming environment R (version 4.0.3) 34 .…”

Section: Methodsmentioning

confidence: 99%

“…The reduction of NBT to formazan upon reacting with ROS/superoxide is evident as a blue precipitate 11 , 12 . The intensity of tissue blue coloration can be quantified to provide a measure for storage related oxidation processes 13 . Fourier transform infrared spectroscopy (FTIR) can be used to detect changes in biochemical composition associated with oxidative damage in tissues and it has the advantage that it requires minimal sample preparation 9 .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Fourier transform infrared spectroscopy coupled with machine learning classification for identification of oxidative damage in freeze-dried heart valves

Liu¹,

Caliskan

Rashidfarokhi³

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

Freeze-drying can be used to ensure off-the-shelf availability of decellularized heart valves for cardiovascular surgery. In this study, decellularized porcine aortic heart valves were analyzed by nitroblue tetrazolium (NBT) staining and Fourier transform infrared spectroscopy (FTIR) to identify oxidative damage during freeze-drying and subsequent storage as well as after treatment with H2O2 and FeCl3. NBT staining revealed that sucrose at a concentration of at least 40% (w/v) is needed to prevent oxidative damage during freeze-drying. Dried specimens that were stored at 4 °C depict little to no oxidative damage during storage for up to 2 months. FTIR analysis shows that fresh control, freeze-dried and stored heart valve specimens cannot be distinguished from one another, whereas H2O2- and FeCl3-treated samples could be distinguished in some tissue section. A feed forward artificial neural network model could accurately classify H2O2 and FeCl3 treated samples. However, fresh control, freeze-dried and stored samples could not be distinguished from one another, which implies that these groups are very similar in terms of their biomolecular fingerprints. Taken together, we conclude that sucrose can minimize oxidative damage caused by freeze-drying, and that subsequent dried storage has little effects on the overall biochemical composition of heart valve scaffolds.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Fourier transform infrared spectroscopy coupled with machine learning classification for identification of oxidative damage in freeze-dried heart valves

Liu¹,

Caliskan

Rashidfarokhi³

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…For the study of the crosslinking agent's impact on the overall secondary protein structure, the spectral fingerprint of all groups was determined. From the acquired spectra, the region of interest (1700-1600 cm −1 ) was selected, which includes absorbance bands (C=O stretching and NH bending) resulting from α-helical (~1650 cm −1 ) and β-sheet (~1630 cm −1 ) structures as described elsewhere [30]. The amide-I band region 1700-1600 cm −1 is used to describe the correlation between the band shape and the secondary structure contents.…”

Section: Protein Fingerprint Region Analysismentioning

confidence: 99%

A Glutaraldehyde-Free Crosslinking Method for the Treatment of Collagen-Based Biomaterials for Clinical Application

Steitz,

Zouhair,

Khan

et al. 2023

Bioengineering

View full text Add to dashboard Cite

Biological bioprostheses such as grafts, patches, and heart valves are often derived from biological tissue like the pericardium. These bioprostheses can be of xenogenic, allogeneic, or autologous origin. Irrespective of their origin, all types are pre-treated via crosslinking to render the tissue non-antigenic and mechanically strong or to minimize degradation. The most widely used crosslinking agent is glutaraldehyde. However, glutaraldehyde-treated tissue is prone to calcification, inflammatory degradation, and mechanical injury, and it is incapable of matrix regeneration, leading to structural degeneration over time. In this work, we are investigating an alternative crosslinking method for an intraoperative application. The treated tissue‘s crosslinking degree was evaluated by differential scanning calorimetry. To confirm the findings, a collagenase assay was conducted. Uniaxial tensile testing was used to assess the tissue’s mechanical properties. To support the findings, the treated tissue was visualized using two-photon microscopy. Additionally, fourier transform infrared spectroscopy was performed to study the overall protein secondary structure. Finally, a crosslinking procedure was identified for intraoperative processing. The samples showed a significant increase in thermal and enzymatic stability after treatment compared to the control, with a difference of up to 22.2 °C and 100%, respectively. Also, the tissue showed similar biomechanics to glutaraldehyde-treated tissue, showing greater extensibility, a higher failure strain, and a lower ultimate tensile strength than the control. The significant difference in the structure band ratio after treatment is proof of the introduction of additional crosslinks compared to the untreated control with regard to differences in the amide-I region. The microscopic images support these findings, showing an alteration of the fiber orientation after treatment. For collagen-based biomaterials, such as pericardial tissue, the novel phenolic crosslinking agent proved to be an equivalent alternative to glutaraldehyde regarding tissue characteristics. Although long-term studies must be performed to investigate superiority in terms of longevity and calcification, our novel crosslinking agent can be applied in concentrations of 1.5% or 2.0% for the treatment of biomaterials.

show abstract

Infrared spectroscopic analysis of hydrogen-bonding interactions in cryopreservation solutions

Caliskan

Oldenhof

Temeloglu

et al. 2023

Biochimica et Biophysica Acta (BBA) - General Subjects

View full text Add to dashboard Cite

Spectroscopic assessment of oxidative damage in biomolecules and tissues

Cited by 9 publications

References 49 publications

Fourier transform infrared spectroscopy coupled with machine learning classification for identification of oxidative damage in freeze-dried heart valves

Fourier transform infrared spectroscopy coupled with machine learning classification for identification of oxidative damage in freeze-dried heart valves

A Glutaraldehyde-Free Crosslinking Method for the Treatment of Collagen-Based Biomaterials for Clinical Application

Infrared spectroscopic analysis of hydrogen-bonding interactions in cryopreservation solutions

Contact Info

Product

Resources

About