Spectroscopic characteristics and energy transfer of bacterial pigment: (pyocyanin/curcumin)

Wadday, Ali Kadhim; Saleh, Zeyad A.; Al-Marjani, Mohammed Fraj

doi:10.1063/1.5141438

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…The absorption spectrum was analyzed in a wavelength range from 295 to 800 nm ( Figure 2 A). The results showed that the absorption spectrum of the exhausted broth of P. aeruginosa GG-7R grown in the absence of spiramycin was similar to that found in other P. aeruginosa strains [ 41 ]. The absorption between 295 and 421 nm and between 548 and 800 nm was markedly reduced when the bacterium was cultured with spiramycin (30 μg/mL).…”

Section: Resultssupporting

confidence: 59%

Spiramycin Disarms Pseudomonas aeruginosa without Inhibiting Growth

et al. 2023

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Spiramycin is a 16-membered macrolide antibiotic currently used in therapy to treat infections caused by Gram-positive bacteria responsible for respiratory tract infections, and it is also effective against some Gram-negative bacteria and against Toxoplasma spp. In contrast, Pseudomonas aeruginosa, which is one of the pathogens of most concern globally, is intrinsically resistant to spiramycin. In this study we show that spiramycin inhibits the expression of virulence determinants in P. aeruginosa in the absence of any significant effect on bacterial multiplication. In vitro experiments demonstrated that production of pyoverdine and pyocyanin by an environmental strain of P. aeruginosa was markedly reduced in the presence of spiramycin, as were biofilm formation, swarming motility, and rhamnolipid production. Moreover, treatment of P. aeruginosa with spiramycin sensitized the bacterium to H2O2 exposure. The ability of spiramycin to dampen the virulence of the P. aeruginosa strain was confirmed in a Galleria mellonella animal model. The results demonstrated that when G. mellonella larvae were infected with P. aeruginosa, the mortality after 24 h was >90%. In contrast, when the spiramycin was injected together with the bacterium, the mortality dropped to about 50%. Furthermore, marked reduction in transcript levels of the antimicrobial peptides gallerimycin, gloverin and moricin, and lysozyme was found in G. mellonella larvae infected with P. aeruginosa and treated with spiramycin, compared to the larvae infected without spiramycin treatment suggesting an immunomodulatory activity of spiramycin. These results lay the foundation for clinical studies to investigate the possibility of using the spiramycin as an anti-virulence and anti-inflammatory drug for a more effective treatment of P. aeruginosa infections, in combination with other antibiotics.

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Section: Resultssupporting

confidence: 59%

Spiramycin Disarms Pseudomonas aeruginosa without Inhibiting Growth

et al. 2023

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“…1 f , E. coli has no absorption band in the visible region. However, there are many bacteria that have inherent absorption bands in the visible region, such as Pseudomonas aeruginosa ( P. aeruginosa ), Staphylococcus aureus ( S. aureus ), Micrococcus luteus ( M. luteus ), and Kocuria oceani ( K. oceani ) 53 – 56 . We consider that by choosing the wavelength of pulsed laser to match the absorption bands, the transient photothermal inactivation of these pathogenic bacteria can be made without the staining treatment.…”

Section: Discussionmentioning

confidence: 99%

Transient photothermal inactivation of Escherichia coli stained with visible dyes by using a nanosecond pulsed laser

Kohmura

Igami

Tatsuno

et al. 2020

Sci Rep

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Efficient inactivation of Escherichia coli (E. coli) under visible (532 nm) pulsed light irradiation was achieved by fusion of a visible light-absorbing dye with E. coli. Inactivation experiments showed that 3-log inactivation of E. coli was obtained within 20 min under a 50 kJ/cm2 dose. This treatment time and dose magnitude were 10 times faster and 100 times lower, respectively, than the values previously obtained by using a visible femtosecond laser. The mechanism of bacterial death was modeled based on a transient photothermal evaporation effect, where a quantitative evaluation of the temperature increase was given based on the heat transfer equation. As a result of this theoretical analysis, the maximum temperature of the bacteria was correlated with the absorption ratio, pulse energy, and surface-to-volume ratio. An increase in the surface-to-volume ratio with the decreasing size of organic structures leads to the possibility of efficient inactivation of viruses and bacteria under low-dose and non-harmful-visible pulsed light irradiation. Hence, this method can be applied in many fields, such as the instantaneous inactivation of pathogenic viruses and bacteria in a safe and simple manner without damaging large organic structures.

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“…This is because the implants are thicker than the tissue section. The strong bacterial TPEF signal was from the pyocyanin pigment (excitation/emission at 360/460 nm 63,64 ), known to be produced by P. aeruginosa, and this was confirmed by imaging bacterial suspension samples 65,66 . The TPEF signal observed in Fig.…”

Section: Discussionmentioning

confidence: 71%

Label-free multimodal imaging of infected Galleria mellonella larvae

Quansah

Ramoji

Thieme

et al. 2022

Sci Rep

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Non-linear imaging modalities have enabled us to obtain unique morpho-chemical insights into the tissue architecture of various biological model organisms in a label-free manner. However, these imaging techniques have so far not been applied to analyze the Galleria mellonella infection model. This study utilizes for the first time the strength of multimodal imaging techniques to explore infection-related changes in the Galleria mellonella larvae due to massive E. faecalis bacterial infection. Multimodal imaging techniques such as fluorescent lifetime imaging (FLIM), coherent anti-Stokes Raman scattering (CARS), two-photon excited fluorescence (TPEF), and second harmonic generation (SHG) were implemented in conjunction with histological HE images to analyze infection-associated tissue damage. The changes in the larvae in response to the infection, such as melanization, vacuolization, nodule formation, and hemocyte infiltration as a defense mechanism of insects against microbial pathogens, were visualized after Enterococcus faecalis was administered. Furthermore, multimodal imaging served for the analysis of implant-associated biofilm infections by visualizing biofilm adherence on medical stainless steel and ePTFE implants within the larvae. Our results suggest that infection-related changes as well as the integrity of the tissue of G. mellonella larvae can be studied with high morphological and chemical contrast in a label-free manner.

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Spectroscopic characteristics and energy transfer of bacterial pigment: (pyocyanin/curcumin)

Cited by 8 publications

References 30 publications

Spiramycin Disarms Pseudomonas aeruginosa without Inhibiting Growth

Spiramycin Disarms Pseudomonas aeruginosa without Inhibiting Growth

Transient photothermal inactivation of Escherichia coli stained with visible dyes by using a nanosecond pulsed laser

Label-free multimodal imaging of infected Galleria mellonella larvae

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