Spectroscopic evaluation of native, milled, and functionalized β-TCP seeding into dental enamel lesions

Karlinsey, Robert L.; Mackey, Allen C.; Walker, Emily R.; Frederick, Katherine E.

doi:10.1007/s10853-009-3774-3

Cited by 13 publications

(17 citation statements)

References 18 publications

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“…In the present study, although the TMR data trends favor the 500 ppm F+fTCP dentifrice, the statistical separation achieved between the two 500 ppm F dentifrices assessed by CSMH suggests the strength of the white-spot enamel framework is affected differently in the presence of fTCP. This view is supported with previous in vitro and in situ observations [10,14,16,19,22].…”

Section: Discussionsupporting

confidence: 91%

“…In particular, there have been several reports on the promising benefits observed through a combination of fluoride and calcium [1,8,[10][11][12]. A unique approach has been through the use of a functionalized β-tricalcium phosphate (fTCP), which is a low-dose calcium phosphate system that cooperates with fluoride to build stronger, more acid-resistant mineral relative to fluoride alone [13][14][15]. Previous studies suggest the calcium oxide polyhedra manifested in the β-tricalcium phosphate lattice, which are protected with specific organic molecules such as fumaric acid or sodium lauryl sulfate, cooperate with fluoride to bond with loosely bound or broken enamel lattice constituents, including orthophosphate [16,17].…”

Section: Introductionmentioning

confidence: 99%

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In situ remineralization of white-spot enamel lesions by 500 and 1,100 ppm F dentifrices

et al. 2011

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The aim of this study was to evaluate the remineralization potential of three silica-containing NaF dentifrice systems in an intraoral model. Subjects (N = 30) in this randomized, three-phase, 28-day, crossover study served as their own control. Each participant wore a customized orthodontic appliance attached to a mandibular molar and contained one tooth block with caries-like lesion. For each phase, participants engaged in twice-daily brushing for 2 min with one of the following dentifrices: 500 ppm F, 500 ppm F plus functionalized β-tricalcium phosphate (fTCP), and a clinically proven 1,100 ppm F. After each phase, appliances were removed, and specimens were analyzed using surface microhardness (SMH), transverse microradiography (TMR), and cross-sectional microhardness (CSMH). Statistically significant (p < 0.05) remineralization of white-spot lesions relative to baseline occurred for each dentifrice as determined with SMH and TMR. No significant differences (p > 0.05) in SMH were found among the three groups, but trending revealed the 500 ppm F plus fTCP produced 26% and 27% greater SMH recovery relative to 500 and 1,100 ppm F, respectively. Similarly, no significant differences (p > 0.05) in TMR were found among the groups. However, the 500 ppm F plus fTCP dentifrice produced 10% and 38% greater mineral recovery relative to 500 and 1,100 ppm F, respectively, while reducing the lesion depth 30% and 52%, respectively. Significant differences (p < 0.05) in CSMH existed among the three dentifice groups at different enamel depths, but statistical differences (p < 0.05) in relative lesion size were only found between 500 ppm F plus fTCP and 500 ppm F. The combination of fTCP and fluoride in a single-compartment, water-based dentifrice can cooperate with fluoride to produce significant remineralization. These results suggest that the combination of 500 ppm F with fTCP may provide comparable anticaries benefits relative to a 1,100 ppm F dentifrice.

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Section: Discussionsupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

In situ remineralization of white-spot enamel lesions by 500 and 1,100 ppm F dentifrices

et al. 2011

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“…An edge-enhanced contrast due to X-ray propagation was used to superpose five groups and to define the first slice depth with respect to the enamel-air interface (Wilkins et al, 1996;Momose, 2005). is suggested based on previous research of surface and subsurface enamel strengthening (Karlinsey and Pfarrer, 2012;Karlinsey et al, 2010b) and further examinations with non-TCP dentifrices, including evaluations of enamel Figure 11. From the top to the bottom shows cross-sectional views of an enamel sample selected randomly for each treatment group of Clinpro Tooth Crème, Clinpro 5000, Tom's of Maine, Sound Enamel, and White-spot Lesions (microscopy on the left, SR micro-CT original view in the middle, and color filtered SR micro-CT used to visualize the lesion depth on the right).…”

Section: Discussionmentioning

confidence: 99%

“…In particular, 3M ESPE's Clinpro Tooth Crème (0.21% NaF) and Clinpro 5000 (1.1% NaF), which contain the TCP ingredient, are two of the newest professional-grade toothpastes commercially available. Inclusion of the functionalized TCP ingredient in NaF formulations has been shown to produce stronger, more acid-resistant mineral relative to fluoride alone in laboratory and clinical evaluations (Karlinsey et al, 2010a(Karlinsey et al, , b, c, 2009a(Karlinsey et al, , b, 2011aAmaechi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

In vitro assessments of white-spot lesions treated with NaF plus tricalcium phosphate (TCP) toothpastes using synchrotron radiation micro computed tomography (SR micro-CT)

Asaizumi¹,

Uesugi²,

Hoshino³

et al. 2014

J. Dent. Oral Hyg.

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Synchrotron radiation micro-computed tomography (SR micro-CT), considered superior to standard polychromatic micro-CT techniques, was used to assess the densities of bovine enamel white-spot lesions (WSL) treated in a 10-day pH cycling model with either: (A) Clinpro Tooth Crème (0.21% NaF plus TCP), (B) Clinpro 5000 (1.1% NaF plus TCP) or (C) Tom's of Maine (0% NaF) dentifrice. Each day consisted of four 2 min treatments, one 4 h acid challenge (pH=5.0), and immersion in artificial saliva (pH=7.0) between these events. After 10 days, WSL specimens were evaluated for lesion depth using confocal microscopy and lesion density using SR micro-CT with depths ranging from 2.76 to 113.16 μm, in 2.76 μm slice increments. Statistical analyses (Student's t-test) were performed at the 95% confidence level. SR micro-CT analyses revealed the NaF plus TCP dentifrices improved WSL densities relative to the fluoride-free toothpaste, and is consistent with an earlier study utilizing polychromatic micro-CT. In contrast to previous findings, SR micro-CT analyses also revealed significant differences in WSL densities treated with the two NaF dentifrices at enamel depths of 13.80, 16.56, and 19.32 μm. These findings suggest SR micro-CT may be especially suited for detecting density differences in lesions sensitive to fluoride-driven remineralization processes.

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“…β-TCP is biocompatible and bioactive (Ghosh et al 2008). The possibility of functionalizing the β-TCP (fTCP) provides a prospective finding in the research of bioactive and fluoride-compatible mineralizing agents: first, it creates barriers that prevent premature fluoride-calcium interactions; second, it facilitates targeted delivery (Karlinsey et al 2008) of calcium and fluoride ions when applied to the teeth via common dental preparations (e.g., dentifrices, mouthrinse, etc.). Studies have demonstrated that fTCP enhances the benefits of fluoride by offering statistically greater remineralization of dental erosion (Amaechi et al 2010;Karlinsey et al 2009;Karlinsey et al 2010a, b;Karlinsey and Pfarrer 2012;Mathews et al 2012).…”

Section: Introductionmentioning

confidence: 99%

The effectiveness of an NaF rinse containing fTCP on eroded enamel remineralization

Oliveira

Mathews²,

Ramalingam³

2016

J Public Health

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Objective The authors investigated the effectiveness of a functionalized tricalcium phosphate (fTCP) combined with a low fluoride level in a mouthrinse to reharden eroded enamel lesions. Methods Ninety enamel slabs attached in pairs to removable appliances were randomly assigned to three treatment groups (n = 30/group): (T1) NaF rinse (225 ppm F + 40 ppm fTCP), (T2) NaF rinse (225 ppm F; ACT ® ), and (T3) no mouthrinse (saliva). While wearing the in situ appliance for 14 days, subjects brushed their teeth with 1100 ppm F toothpaste (Crest©) for 2 min, rinsed with 15 ml of water for 10s, and then rinsed with 15 ml of their assigned treatment mouthrinse. Treatment efficacy was evaluated using surface microhardness (SMH) and transverse microradiography (TMR). Intra-and intergroup comparisons (α = 0.05) were performed using the t-test and ANOVA followed by the Tukey test (HSD). Results With SMH, intragroup comparison (t-test) indicated significant rehardening of the eroded lesion with exposure to T1 (p < 0.001) and T2 (p < 0.01) but not with T3 However, with TMR, remineralization was only significant (p = 0.01) with T1, but not with T2 and T3. In the intergroup comparison with percentage change in SMH, T1 was significantly different from T3 (p < 0.01; Tukey HSD) but not from T2, and T2 was significantly different from T3. Intergroup comparison based on percentage mineral gain showed that T2 (p = 0.02) and T3 (p = 0.01) differed significantly from fTCP, but not between each other. Conclusion Addition of low level fluoride to functionalized β-tricalcium phosphate promoted rehardening of eroded enamel lesions.

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Spectroscopic evaluation of native, milled, and functionalized β-TCP seeding into dental enamel lesions

Cited by 13 publications

References 18 publications

In situ remineralization of white-spot enamel lesions by 500 and 1,100 ppm F dentifrices

In situ remineralization of white-spot enamel lesions by 500 and 1,100 ppm F dentifrices

In vitro assessments of white-spot lesions treated with NaF plus tricalcium phosphate (TCP) toothpastes using synchrotron radiation micro computed tomography (SR micro-CT)

The effectiveness of an NaF rinse containing fTCP on eroded enamel remineralization

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