Spectroscopic Microvascular Blood Detection From the Endoscopically Normal Colonic Mucosa: Biomarker for Neoplasia Risk

Roy, Hemant K.; Gomes, Andrew; Turzhitsky, Vladimir; Goldberg, Michael J.; Rogers, Jeremy D.; Ruderman, Sarah; Young, Kim Lea; Kromine, Alex; Brand, Randall E.; Jameel, Mohammed; Vakil, Parmede; Hasabou, Nahla; Backman, Vadim

doi:10.1053/j.gastro.2008.06.046

Cited by 61 publications

(60 citation statements)

References 30 publications

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“…[6][7][8][9][10] These techniques are able to provide information regarding the tissue substructure, which can serve as biomarkers for the presence of intraepithelial neoplasia. Recently, Backman and Roy have proposed measurement of the field effect of colon carcinogenesis using light scattering techniques as a means of risk stratification and prescreening for CRC.…”

Section: Introductionmentioning

confidence: 99%

Detection of intestinal dysplasia using angle-resolved low coherence interferometry

et al. 2011

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Abstract. Angle-resolved low coherence interferometry (a/LCI) is an optical biopsy technique that allows for depth-resolved, label-free measurement of the average size and optical density of cell nuclei in epithelial tissue to assess the tissue health. a/LCI has previously been used clinically to identify the presence of dysplasia in Barrett's Esophagus patients undergoing routine surveillance. We present the results of a pilot, ex vivo study of tissues from 27 patients undergoing partial colonic resection surgery, conducted to evaluate the ability of a/LCI to identify dysplasia. Performance was determined by comparing the nuclear morphology measurements with pathological assessment of co-located physical biopsies. A statistically significant correlation between increased average nuclear size, reduced nuclear density, and the presence of dysplasia was noted at the basal layer of the epithelium, at a depth of 200 to 300 μm beneath the tissue surface. Using a decision line determined from a receiver operating characteristic, a/LCI was able to separate dysplastic from healthy tissues with a sensitivity of 92.9% (13/14), a specificity of 83.6% (56/67), and an overall accuracy of 85.2% (69/81). The study illustrates the extension of the a/LCI technique to the detection of intestinal dysplasia, and demonstrates the need for future in vivo studies. C 2011 Society of Photo-Optical Instrumentation Engineers (SPIE).

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Section: Introductionmentioning

confidence: 99%

Detection of intestinal dysplasia using angle-resolved low coherence interferometry

et al. 2011

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“…A field effect has been noted in light-scattering spectroscopy of the colon. 54 As the number of dysplastic ovaries in this study was small, however, a firm conclusion cannot be drawn.…”

Section: Discussionmentioning

confidence: 75%

Analysis of second-harmonic-generation microscopy in a mouse model of ovarian carcinoma

et al. 2012

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“…Hemoglobin concentration measured from this depth was diagnostic for early detection of colonic neoplasia. 2 For simplicity, we will maintain the same optical properties as the first case study since the optical properties of the colon have been found to be similar. 49 In general, the optical properties will need to be adjusted based on the tissue or organ being investigated.…”

Section: Case Studiesmentioning

confidence: 99%

“…Our optical and histological investigations in human patients and animal models of colon carcinogenesis have shown diagnostic alterations in hemoglobin concentration that are present in the mucosa but attenuated at deeper submucosal depths. [1][2][3][4][5] Studies with angle-resolved low-coherence interferometry demonstrate that elevation of nuclear diameter associated with dysplasia in Barrett's esophagus was only detectable at a depth of 200 to 300 μm and not observable at 0 to 100 μm or 100 to 200 μm. 6 Several fiber-optic probe modalities have been developed to target the mucosal layer with a wide field of view.…”

Section: Introductionmentioning

confidence: 99%

“…[12][13][14] These methods have proven successful in diagnosing epithelial lesions including those in the colon, oral, and other types of mucosae. 1,2,9,13,[15][16][17][18] On the other hand, diffuse reflectance spectroscopy (DRS) methods typically have a sampling depth on the order of several millimeters and are consequently not very selective to the mucosal layer. However, the deeper sampling depth of DRS may be advantageous for other applications.…”

Section: Introductionmentioning

confidence: 99%

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Algorithm for automated selection of application-specific fiber-optic reflectance probes

Gomes¹,

Backman²

2013

J. Biomed. Opt

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Abstract. Several optical techniques and fiber-optic probe systems have been designed to measure the optical properties of tissue. While a wide range of options is often beneficial, it poses a problem to investigators selecting which method to use for their biomedical application of interest. We present a methodology to optimally select a probe that matches the application requirements. Our method is based both on matching a probe's mean sampling depth with the optimal diagnostic depth of the clinical application and on choosing a probe whose interrogation depth and path length is the least sensitive to alterations in the target medium's optical properties. Satisfying these requirements ensures that the selected probe consistently assesses the relevant tissue volume with minimum variability. To aid in probe selection, we have developed a publicly available graphical user interface that takes the desired sampling depth and optical properties of the medium as its inputs and automatically ranks different techniques in their ability to robustly target the desired depth. Techniques investigated include single fiber spectroscopy, differential path length spectroscopy, polarization-gating, elastic light scattering spectroscopy, and diffuse reflectance. The software has been applied to biological case studies.

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Spectroscopic Microvascular Blood Detection From the Endoscopically Normal Colonic Mucosa: Biomarker for Neoplasia Risk

Cited by 61 publications

References 30 publications

Detection of intestinal dysplasia using angle-resolved low coherence interferometry

Detection of intestinal dysplasia using angle-resolved low coherence interferometry

Analysis of second-harmonic-generation microscopy in a mouse model of ovarian carcinoma

Algorithm for automated selection of application-specific fiber-optic reflectance probes

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