Spectroscopic, structural and thermodynamic properties of chlorpyrifos bound to serum albumin: A comparative study between BSA and HSA

Han, Xiaojiao; Tian, Fang; Ge, Yushu; Jiang, Feng-Lei; Lai, Lu; Li, Dongwei; Yu, Qiuliyang; Wang, Jia; Chen, Lin; Liu, Yi

doi:10.1016/j.jphotobiol.2011.12.010

Cited by 183 publications

(43 citation statements)

References 29 publications

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“…In the absence or presence of Pd(II) complexes, the fluorescence intensity ratios of peak B and peak C are 1.59:1, 1.78:1 for complex I and 2.05:1, 1.88:1 for complex II, respectively. The above phenomena and the analysis of the fluorescence characteristic of the peaks revealed that binding of Pd(II) complexes with HSA induced a slight unfolding of the polypeptide and some micro environmental changes of HSA when the binding between HSA and Pd(II) complexes formed and the binding site was near tryptophan and tyrosine residues on HSA and Pd(II) complexes [52][53][54][55].…”

Section: Three-dimensional Fluorescencementioning

confidence: 99%

Biophysical study on the interaction between two palladium(II) complexes and human serum albumin by Multispectroscopic methods

Saeidifar

Mansouri‐Torshizi

Saboury

2015

Journal of Luminescence

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Section: Three-dimensional Fluorescencementioning

confidence: 99%

Biophysical study on the interaction between two palladium(II) complexes and human serum albumin by Multispectroscopic methods

Saeidifar

Mansouri‐Torshizi

Saboury

2015

Journal of Luminescence

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“…The fluorescence Figure 1. Structures of carbendazim and chlorpyrifos spectra revealed that chlorpyrifos causes the quenching of the fluorescence emission of serum albumin which determined at (λex: 275nm and λem: 350nm) as reported by Han et al [17]. A numerous publications and research papers focus on separation methods to detect carbendazim and chlorpyrifos for simultaneous determination using RP-HPLC-UV/Vis [23,25] and LC-MS-MS [7].…”

Section: Introductionmentioning

confidence: 66%

“…Unfortunately, Chlorpyrifos with a thiophosphoryl (P=S) functional group is important causes of morbidity and mortality following intentional self-poisoning or in cases of occupational or environmental exposure [1,5,16,17]. The American Department of Pesticide Regulation (ADPR) gave chlorpyrifos a high priority for risk assessment for its acute toxicity [18].…”

Section: Introductionmentioning

confidence: 99%

“…The American Department of Pesticide Regulation (ADPR) gave chlorpyrifos a high priority for risk assessment for its acute toxicity [18]. Recently studies have found that carbendazim cause infertility and destroy the testicles of human males [19,20], and Chlorpyrifos cause lung cancer [15,21,22] and acts by interfering with cholinesterase, an enzyme that is essential for the proper working of the nervous system of both humans and insects [1,5,15,17,18,23]. Effects of carbendazim and chlorpyrifos were seen in liver, thyroid, thymus and blood in the combination groups [23].…”

Section: Introductionmentioning

confidence: 99%

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Determination of Carbendazim and Chlorpyrifos in Selected Fruits and Vegetables Samples Using QuEChERS-HPLC-FD

Hazer¹,

Akkbik²,

Demir³

et al. 2016

Eurasian J Anal Chem

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A new method was developed to determine pesticide residues in fruit and vegetable extracts with short time analysis. An optimum results were achieved using binary mobile phase consisting of (methanol: water; 95:5, v/v) and fluorescence detection (λEx and λEm set at 280 and 340 nm, respectively) was used. The dynamic range was between 0.100 to 10 mg L -1 with relative standard deviation less than 0.45%, (n=4). Limits of detection and recoveries for carbendazim and chlorpyrifos were 0.073 mg L -1 (84.2-106.5%) and 0.062 mg L -1 (85.7-90.3%), respectively. The results revealed that the concentrations of carbendazim and chlorpyrifos residue in all collected samples were below than the EU legal limit.

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“…Using the HSA modified gold electrode, the interaction of the drugs and HSA was studied by measuring the peak current of electrolyte solution (Han et al, 2012). The peak current decreased with the addition of both drugs.…”

Section: Discussionmentioning

confidence: 99%

Antifungal efficiency of miconazole and econazole and the interaction with transport protein: A comparative study

Zhang

Cheng

2014

Pharmaceutical Biology

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Context: Miconazole (MIZ) and econazole (ECZ) are clinically used as antifungal drugs. Objective: The drug effect and binding property with transport protein human serum albumin of MIZ and ECZ were studied. Materials and methods: The antifungal efficiency was investigated by microdiluting drug solutions from 0 to 48 mmol L À1 through microcalorimetry and voltammetry studies. Transmission electron microscopy was used for morphological observations of C. albicans. The interaction with HSA was studied by electrochemical methods, fluorescence spectrometry, electron microscopy, and molecular simulation. , the peak currents increased to 4.887 and 6.024 mA. The peak currents of C. albicans in the presence of 20 and 48 mmol L À1 ECZ were 4.701 and 5.544 mA. The docking scores for MIZ and ECZ of the best binding conformation in site I and site II were 5.60, 4.79, 5.63, and 5.85. Discussion and conclusion: Strong inhibition to the metabolism of C. albicans and destructive effect was proved for both drugs. The lower IC 50 , growth rate constant of C. albicans, and higher peak current, reveal stronger antifungal activity of MIZ. Both drugs show an efficient quenching effect to intrinsic fluorescence residues of protein. MIZ mainly binds on site I while ECZ on site II. Molecular modeling experiments give further insight of the binding mechanism.

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Spectroscopic, structural and thermodynamic properties of chlorpyrifos bound to serum albumin: A comparative study between BSA and HSA

Cited by 183 publications

References 29 publications

Biophysical study on the interaction between two palladium(II) complexes and human serum albumin by Multispectroscopic methods

Biophysical study on the interaction between two palladium(II) complexes and human serum albumin by Multispectroscopic methods

Determination of Carbendazim and Chlorpyrifos in Selected Fruits and Vegetables Samples Using QuEChERS-HPLC-FD

Antifungal efficiency of miconazole and econazole and the interaction with transport protein: A comparative study

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