Spectroscopic study of the reaction mechanism of buspirone interaction with iodine and tetracyanoethylene reagents and its applications

Abstract

The reactions between the drug buspirone (busp) in its base form and iodine amphoteric reagent (n-donor and/or sigma-acceptor) and with tetracyanoethylene as a pi-acceptor reagent (TCNE) have been studied spectrophotometrically at different reactant concentrations, time intervals, temperatures, and with different solvents and wavelengths, with the aim of selecting the conditions that give the most suitable molar extinction coefficients. This study aims chiefly to throw light on the nature of these reactions and to select the most proper conditions for spectrophotometric application of these reagents to determine this biologically active drug used in treating different diseases. The reaction mechanism involves the formation of busp-I(2) outer and inner sphere complexes. The separated busp-I(2) solid product obtained was investigated using elemental analyses, FT-IR, thermal analyses (TA) and electron ionization mass spectrometry (EI-MS) and was found to be biologically active. The reaction mechanism of busp-TCNE involves the formation of a charge transfer (CT) complex. The analytical parameters of the proposed spectrophotometric procedures were calculated. These procedures were applied in the analysis of busp in its formulations as a drug used to treat psychiatric illnesses. The values of the Sandell sensitivity, standard deviation (SD), relative standard deviation (RSD) and recovery percentage show the high sensitivity of these procedures. This study also presents a promising new busp-I(2) drug derivative that can be used more efficiently for the same purposes as its parent. It gives a clear idea about the possible metabolites and metabolic pathways of busp and its derivative that may occur in vivo.

Analytical Determination of Buspirone Hydrochloride in Pure Solutions, Pharmaceutical Preparation and Urine Samples

“…The optimum pH ranges for complete formation of the ion-associates which showed the highest absorbance values, at their respective λ max were (3-5), (4)(5), (3)(4)(5)(6) and (3-6), for Bu-HCl ion associates with PA, BBT, AR and BPR respectively as shown in (Figure 2). From the results shown in (Figure 2) we can observe that there are suiTable ranges of stability of the absorbances of the drug-coloring reagent ion-associates which indicates that small change in pH will not affect on the efficiency of the formation of ion associates.…”

“…Several analytical methods have been applied to determine Buspirone hydrochloride (Bu-HCl) quantitatively in their dosage forms including spectrophotometric method [2][3][4][5], liquid chromatography mass spectrometry LC/MS [6][7][8][9], High Performance Liquid Chromatogrphy HPLC [10][11][12][13][14][15][16][17][18], gas chromatography [19] gas chromatography mass spectrometry GCMS [20][21][22], ion selective electrode [20][21][22][23] and potentiometry [24][25][26].…”

Insights into the binding of buspirone to human serum albumin using multi-spectroscopic and molecular docking techniques

“…Buspirone hydrochloride (BSH), an azaspirodecanedione compound, is an anxiolytic drug and partial serotonin receptor agonist used to manage anxiety disorders and alleviate symptoms [ 1 , 2 ]. It seems that its anxiolytic effects are related to its effect on the neurotransmitter serotonin (5-HT) [ 3 ].…”

Aggregation of Gold Nanoparticles for Spectrophotometric Determination of Bisoprolol Hemifumarate, Buspirone HCl and Doxazosin Mesylate

“…Various analytical methods have been employed for its determination in raw material, pharmaceuticals and biological fluids. These methods include chromatography [10], spectrofluorimetry [11] and spectrophotometry [12][13][14]. .…”