“…Using images obtained by specular microscopy, it is possible to examine the corneal endothelium and obtain data related to endothelial cell density and morphology. This technique has been used to quantify endothelial parameters in humans and other animal species including pigs (TAMAYO-ARANGO et al, 2009), rabbits (SAILSTAD & PEIFFER, 1981;OJEDA et al, 2001), dogs (GWIN et al, 1982;PIGATTO et al, 2006;RODRIGUES et al, 2006), horses (ANDREW et al, 2001;LEDBETTER & SCARLETT, 2009), llamas and alpacas (ANDREW et al, 2002), chinchillas (BERCHT et al, 2015), and cats (FRANZEN et al, 2010), among others. The high cost of specular microscopes and the difficulty in obtaining good images in injured endothelial areas are common challenges or barriers to the use of this technique (ANDREW et al, 2001;PIGATTO et al, 2005a;SAAD et al, 2008).…”