Sperm‑associated antigen 5 is a potential biomarker for poor prognosis in breast cancer

Zhou, Xiaoli; Jia, Lizhou; Sun, Yangyang; Xu, Lingyun; Wang, Xudong; Tang, Qi

doi:10.3892/ol.2018.9729

Cited by 6 publications

(9 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Protein expression was visualized under a microscope (Olympus Corp. Tokyo, Japan), and photos were taken for subsequent analyses. Histopathological evaluations were performed as described previously 28…”

Section: Methodsmentioning

confidence: 99%

<p>Osteoglycin (OGN) Inhibits Cell Proliferation and Invasiveness in Breast Cancer via PI3K/Akt/mTOR Signaling Pathway</p>

Zhang

Dong

et al. 2019

OTT

View full text Add to dashboard Cite

IntroductionPrevious studies have indicated that the small leucine-rich proteoglycan (SLR) osteoglycin (OGN) is downregulated in various cancers, including squamous cervical carcinoma, gastric cancer, and colorectal adenoma, indicating that OGN is a putative tumor suppressor. However, its exact role in the pathology of human cancers, especially breast cancer (BC), is not clear.MethodsThe expression of OGN in BC tissues was examined using qRT-PCR. Online databases were employed to analyze the correlation between OGN expression and clinicopathological characteristics. CCK-8 assay, colony formation assay, transwell migration and invasion assays were applied to detect cell proliferation, colony formation, migration and invasion of BC cells, respectively. Xenograft tumor models were constructed to explore the role of OGN on tumor growth in vivo.ResultsOGN expression was reduced in 24 paired BC samples compared with normal tissue. Decreased expression of OGN was correlated with greater pathological grade, a more aggressive tumor subtype, and poor overall survival. In vitro experiments showed that OGN overexpressed by plasmid transfection significantly inhibited cell proliferation, colony formation, migration, and invasion of BC cell lines. In xenograft tumor models, overexpression of OGN repressed the growth of MCF-7 cells in vivo and alleviated the compression of the tumor on surrounding structures. We also observed that OGN expression reversed EMT via repressing the PI3K/Akt/mTOR pathway.ConclusionThis study revealed that OGN could function as a tumor suppressor during breast carcinogenesis, and we contribute new evidence to the body of research on the SLRP family.

show abstract

Section: Methodsmentioning

confidence: 99%

<p>Osteoglycin (OGN) Inhibits Cell Proliferation and Invasiveness in Breast Cancer via PI3K/Akt/mTOR Signaling Pathway</p>

Zhang

Dong

et al. 2019

OTT

View full text Add to dashboard Cite

show abstract

“…Sperm-associated antigen 5 (SPAG5), primarily described as a component of the mitotic spindle required for entry into anaphase [7][8][9][10][11][12], acts as a driver oncogene in various cancer types [13][14][15][16][17], including triple-negative breast cancer [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation

Canu¹,

Donzelli²,

Sacconi³

et al. 2020

Cell Death Differ

View full text Add to dashboard Cite

Sperm-associated antigen 5 (SPAG5) is an important driver of the cell mitotic spindle required for chromosome segregation and progression into anaphase. SPAG5 has been identified as an important proliferation marker and chemotherapy-sensitivity predictor, especially in estrogen receptor-negative breast cancer subtypes. Here, we report that SPAG5 is a direct target of miR-10b-3p, and its aberrantly high expression associates with poor disease-free survival in two large cohorts of breast cancer patients. SPAG5 depletion strongly impaired cancer cell cycle progression, proliferation, and migration. Interestingly, high expression of SPAG5 pairs with a YAP/TAZ-activated signature in breast cancer patients. Reassuringly, the depletion of YAP, TAZ, and TEAD strongly reduced SPAG5 expression and diminished its oncogenic effects. YAP, TAZ coactivators, and TEAD transcription factors are key components of the Hippo signaling pathway involved in tumor initiation, progression, and metastasis. Furthermore, we report that SPAG5 is a direct transcriptional target of TEAD/YAP/TAZ, and pharmacological targeting of YAP and TAZ severely reduces SPAG5 expression. Collectively, our data uncover an oncogenic feedback loop, comprising miR-10b-3p, SPAG5, and YAP/TAZ/TEAD, which fuels the aberrant proliferation of breast cancer.

show abstract

“…Sperm‐associated antigen 5 (SPAG5), also termed mitotic spindle‐associated protein 5, was first identified in 2001. SPAG5 can bind to microtubules and influences the separation of sister chromatids by regulating spindles, thus affecting the cell cycle.…”

Section: Introductionmentioning

confidence: 99%

Expression, immune infiltration and clinical significance of SPAG5 in hepatocellular carcinoma: A gene expression‐based study

Chen

et al. 2020

The Journal of Gene Medicine

View full text Add to dashboard Cite

Background Overexpression of sperm‐associated antigen 5 (SPAG5) is a marker of poor prognosis in numerous tumors and is recognized as an index of tumor proliferation; however, its expression in liver cancer remains unclear. Methods The Oncomine (https://www.oncomine.org) and Timer (https://cistrome.shinyapps.io/timer) databases were used to analyze the expression of SPAG5 in liver hepatocellular carcinoma (HCC) and normal liver tissues. The relationship between the expression of SPAG5 and immune infiltration of HCC was investigated using the Timer and GEPIA (http://gepia.cancer‐pku.cn) databases, and the mechanism was analyzed using Gene Set Enrichment Analysis. A Kaplan–Meier Plotter (http://kmplot.com/analysis) was used to evaluate the effect of SPAG5 on the prognosis of patients with HCC. Results The results revealed that the SPAG5 expression level was positively correlated with the infiltration levels of CD8+ T cells, macrophages, neutrophils, and especially B cells and dendritic cells. In addition, SPAG5 expression was significantly associated with T cell exhaustion. The overall survival time, progression‐free survival time, recurrence‐free survival time and disease‐specific survival time were significantly reduced for HCC patients with high SPAG5 expression (p < 0.01) and high expression of SPAG5 was significantly associated with a poor overall survival time and progression‐free survival time of grade and stage II–III HCC (p < 0.05) but not with stage I HCC (p > 0.05). Additionally, the expression of SPAG5 is related to the p53 and cell cycle signal pathways. Conclusions In conclusion, SPAG5 is not only a marker of immune infiltration and poor prognosis, but also a potential therapeutic target for liver cancer.

show abstract

Sperm‑associated antigen 5 is a potential biomarker for poor prognosis in breast cancer

Cited by 6 publications

References 25 publications

<p>Osteoglycin (OGN) Inhibits Cell Proliferation and Invasiveness in Breast Cancer via PI3K/Akt/mTOR Signaling Pathway</p>

<p>Osteoglycin (OGN) Inhibits Cell Proliferation and Invasiveness in Breast Cancer via PI3K/Akt/mTOR Signaling Pathway</p>

Aberrant transcriptional and post-transcriptional regulation of SPAG5, a YAP-TAZ-TEAD downstream effector, fuels breast cancer cell proliferation

Expression, immune infiltration and clinical significance of SPAG5 in hepatocellular carcinoma: A gene expression‐based study

Contact Info

Product

Resources

About