Sperm morphology of beef bulls evaluated by two different methods

Freneau, Gustavo Eduardo; Chenoweth, Peter J.; Ellis, R. W.; Rupp, Gary P.

doi:10.1016/j.anireprosci.2009.08.015

Cited by 54 publications

(41 citation statements)

References 22 publications

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“…This disqualification index is justified by the fact that the evaluated animals were in the phase of transition to sexual maturity characterized by the physical aspects (rectilinear sperm motility, vigor, and turbulence) and morphological properties of the semen as found in the present experiment (Table 1). The values obtained, presented low standard deviation and are similar to those described by Brito et al (2004), Freneau et al (2006), and Freneau et al (2010), who also used young Nelore bulls in their experiments.…”

Section: Resultssupporting

confidence: 73%

Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls

Cardeal

Alberton

Boscarato

et al. 2017

SCA

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Determination of sperm concentration using the Neubauer chamber (hemocytometric method) is a direct method for counting cells and also the most reliable. However, the process is time-consuming rendering it the least practical method when large numbers of ejaculates need to be processed. The spectrophotometer measures sperm concentrations as optical density and its main advantages are practicality and speed. This paper aimed to compare the results between evaluators using the hemocytometric method and the spectrophotometer for measuring sperm concentrations in young Nelore bulls. In total, 73 ejaculations from 20 young Nelore bulls were collected by electroejaculation. After soundness examination, 10 µL of the semen was diluted in 2 mL saline formaldehyde for measuring the sperm concentration per mL by the hemocytometric method (measured by three different evaluators) and the spectrophotometer method at 550 nm wavelength. No differences were detected in the results of sperm concentration measurements per mL among the evaluators using the hemocytometric method and the spectrophotometer (P > 0.05). The intraclass correlation was high (0.9), showing high replicability among the evaluator measurements. These results demonstrate that measurements performed using the spectrophotometer are reliable and can substitute the hemocytometric method in future for performing sperm concentration measurements in young Nelore bulls, thus improving and standardizing the techniques used in andrology laboratories. Key words: Absorbance. Andrology. Bos indicus. Neubauer chamber. Semen. ResumoA determinação da concentração espermática pela câmara de Neubauer (método hematocitométrico) é um método direto para contar células, e também é o mais confiável. Entretanto, o processo é demorado, o que o torna um método menos prático quando a quantidade de ejaculados a ser processado é muito grande. O espectrofotômetro é um dispositivo que mede a concentração de espermatozoides através

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Section: Resultssupporting

confidence: 73%

Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls

Cardeal

Alberton

Boscarato

et al. 2017

SCA

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“…Eosin-nigrosin staining is also recommended by WHO as a basic method for evaluating human sperm morphology (Björndahl et al 2003(Björndahl et al , 2004. Apart from sperm morphology assessments, this method also enables the identification of living and dead sperm (Sprecher & Coe 1996, Zambelli & Cunto 2006, Freneau et al 2010. Nevertheless, both staining methods allow only for observation of the spermatozoon as a uniform structure and enable measurements of only the sperm head and tail.…”

Section: Discussionmentioning

confidence: 99%

Comparison of different chromatin staining techniques for bull sperm

Andraszek¹,

Banaszewska

Czubaszek³

et al. 2014

Arch. Anim. Breed.

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Morphological analysis of semen is a very important step in fertility assessment, but many semen defects are not detectable at the morphological level. These include pathological changes in sperm chromatin structure. During mammalian spermiogenesis, histone proteins associated with DNA structure are replaced by specific protamines, with which chromatin does not form nucleosomal complexes. In the fully developed, mature sperm, the histones are replaced with protamines. Disruptions of nucleoprotein structure can be treated as possible indicators of the biological value of spermatozoa. The experimental material consisted of sperm from one-and-a-half-year-old bulls, isolated post mortem from the tail of the epididymis. The smears were stained with silver nitrate (AgNO 3 ), acridine orange (AO), aniline blue (AB) and chromomycin A3 (CMA3). Sperm dimensions largely depend on individual variability among the bulls. In most cases, differences in sperm dimensions were identified between individuals, which was confirmed in the statistics. Sperm with elevated, abnormal histone levels were proportionally quite scarce (1.4 %). Studies of nuclear proteins in the context of infertility demonstrate the important influence of normal chromatin structure on sperm functions.

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“…These abnormalities vary from morphological defects that are evident on clinical examination to those which are more subtly defective (Chenoweth et al 2005). An understanding of the significance of specific types of sperm abnormalities and the numbers in which these are detected in the ejaculate allows the diagnostician to make a prognosis of fertility and might indicate a course of treatment to assist in the recovery of a male with abnormal sperm production (Freneau et al 2010). It should be noted that there is currently a substantial amount of support for the recommendation that the minimum percentage of morphologically normal sperm in an ejaculate should lie in the range of 70-80% in order to achieve optimum fertility (Kuster et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

et al. 2013

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Pesq. Vet. Bras. 33 (7) The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm.

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Sperm morphology of beef bulls evaluated by two different methods

Cited by 54 publications

References 22 publications

Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls

Comparative study between the hemocytometric and spectrophotometric methods for measuring the sperm concentration of young Nelore bulls

Comparison of different chromatin staining techniques for bull sperm

Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

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