Sperm motility modulated by Trpv1 regulates zebrafish fertilization

Chen, Ying; Wang, Han-Tsing; Wang, Fang; Chen, Chen; Zhang, Peng; Song, Dandan; Luo, Tao; Xu, Hong; Zeng, Xuhui

doi:10.1016/j.theriogenology.2020.03.032

Cited by 14 publications

(18 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a study of de Toni et al [42], human spermatozoa migrating towards a temperature gradient of 31-37 • C showed higher levels of TRPV1 at the protein and mRNA levels. In zebrafish, a targeted blockage of TRPV1 resulted in a suppression of sperm motility and its recovery after channel reactivation [43]. Accordingly, in Labeo rohita, the activation of TRPV1 by endogenous activator N-arachidonoyl dopamine (NADA) improved the quality and duration of sperm motility [40].…”

Section: Transient Receptor Potential Vanilloid (Trpv)mentioning

confidence: 99%

“…Experiments with the blocking and activation of TRPV1 have indicated that this ion channel not only activates basic and hyperactivated motility, but also capacitation and acrosome reactions in boar spermatozoa [44]. The immunostaining of TRPV1 has indicated this channel to be localized in the sperm head, the acrosome, neck, and flagellum [40,[42][43][44]. The activation of the TRPV4 channel depends on the extracellular osmolality, mechanical stimuli (e.g., membrane stretching and shear stress), pH, and lipids [36,45].…”

Section: Transient Receptor Potential Vanilloid (Trpv)mentioning

confidence: 99%

See 1 more Smart Citation

Structure and Function of Ion Channels Regulating Sperm Motility—An Overview

Nowicka-Bauer

Szymczak-Cendlak

2021

IJMS

View full text Add to dashboard Cite

Sperm motility is linked to the activation of signaling pathways that trigger movement. These pathways are mainly dependent on Ca2+, which acts as a secondary messenger. The maintenance of adequate Ca2+ concentrations is possible thanks to proper concentrations of other ions, such as K+ and Na+, among others, that modulate plasma membrane potential and the intracellular pH. Like in every cell, ion homeostasis in spermatozoa is ensured by a vast spectrum of ion channels supported by the work of ion pumps and transporters. To achieve success in fertilization, sperm ion channels have to be sensitive to various external and internal factors. This sensitivity is provided by specific channel structures. In addition, novel sperm-specific channels or isoforms have been found with compositions that increase the chance of fertilization. Notably, the most significant sperm ion channel is the cation channel of sperm (CatSper), which is a sperm-specific Ca2+ channel required for the hyperactivation of sperm motility. The role of other ion channels in the spermatozoa, such as voltage-gated Ca2+ channels (VGCCs), Ca2+-activated Cl-channels (CaCCs), SLO K+ channels or voltage-gated H+ channels (VGHCs), is to ensure the activation and modulation of CatSper. As the activation of sperm motility differs among metazoa, different ion channels may participate; however, knowledge regarding these channels is still scarce. In the present review, the roles and structures of the most important known ion channels are described in regard to regulation of sperm motility in animals.

show abstract

Section: Transient Receptor Potential Vanilloid (Trpv)mentioning

confidence: 99%

Section: Transient Receptor Potential Vanilloid (Trpv)mentioning

confidence: 99%

Structure and Function of Ion Channels Regulating Sperm Motility—An Overview

Nowicka-Bauer

Szymczak-Cendlak

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…Embryos and larvae subjected to nanocomposite treatment at 1000 µg/mL and raised for reproduction after month six showed an average fertility rate of 40.3%, defined as the number of viable embryos (Nv) at 24 hpf over the total number of eggs collected (Nt), calculated using the formula (Nv*100%)/Nt [ 34 ]. The control group raised under the same conditions had an average fertility rate of 33%.…”

Section: Resultsmentioning

confidence: 99%

Toxicity of Modified Magnetite-Based Nanocomposites Used for Wastewater Treatment and Evaluated on Zebrafish (Danio rerio) Model

et al. 2022

View full text Add to dashboard Cite

Magnetite-based nanocomposites are used for biomedical, industrial, and environmental applications. In this study, we evaluated their effects on survival, malformation, reproduction, and behavior in a zebrafish animal model. Nanoparticles were synthesized by chemical coprecipitation and were surface-functionalized with (3-aminopropyl) triethoxysilane (APTES), L-cysteine (Cys), and 3-(triethoxysilyl) propylsuccinic anhydride (CAS). All these nanocomposites were designed for the treatment of wastewater. Zebrafish embryos at 8 h post-fertilization (hpf) and larvae at 4 days post-fertilization (dpf) were exposed to the magnetic nanocomposites Fe3O4 MNP (magnetite), MNP+APTES, MNP+Cys, MNP+APTES+Cys, and MNP+CAS, at concentrations of 1, 10, 100, and 1000 µg/mL. Zebrafish were observed until 13 dpf, registering daily hatching, survival, and malformations. Behavior was tested at 10 dpf for larvae, and reproduction was analyzed later in adulthood. The results showed that the toxicity of the nanocomposites used were relatively low. Exploratory behavior tests showed no significant changes. Reproduction in adults treated during development was not affected, even at concentrations above the OECD recommendation. Given the slight effects observed so far, these results suggest that nanocomposites at the concentrations evaluated here could be a viable alternative for water remediation because they do not affect the long-term survival and welfare of the animals.

show abstract

“…This includes aquaporins that facilitate the efflux of water for motility activation (Boj et al, 2015;Chauvigné et al, 2013), or the mitochondrial efflux of hydrogen peroxide for the maintenance of ATP production and flagellar contractions (Chauvigné et al, 2015(Chauvigné et al, , 2021. Other upregulated genes encode ion channels involved in sperm motility, such as Trpv1 (Majhi et al, 2013;Chen et al, 2020) and different cyclic nucleotide-gated channels (Fechner et al, 2015), or which are potentially implicated in cell volume regulation, such as Vdac1 (Triphan et al, 2008), Lrrc8d (Jentsch, 2016) and Trpv4 (Benfenati et al, 2011). Each of these two processes is considered important for the activation and maintenance of sperm motility in marine teleosts (Boj et al, 2015;Alavi et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Protamine lacking piscine spermatozoa are transcriptionally active

Castro-Arnau

Chauvigné

Gómez‐Garrido

et al. 2021

Preprint

View full text Add to dashboard Cite

Transcriptional quiescence of post-meiotic spermatozoa associated with protamine-mediated chromatin condensation is widely recognized in animals. How sperm acquire the extratesticular maturational competence to move and fertilize the egg is therefore thought to occur via non-transcriptional mechanisms. Here, using transcriptional profiling during spermatozoon differentiation in a fish that does not condense chromatin with protamines, we uncover spatially distinct roles of the GnRH receptor and PDGF signaling pathways between the somatic epithelia of the extratesticular ducts and the maturing spermatozoa. In vitro induction and inhibition experiments demonstrate that the endocrine signaling pathways are conserved in different lineages of fish and activate de novo transcription of spermatozoon genes required for the acquisition of full motility. These experiments further confirmed that mitochondrial translation is important for sperm maturation in anamniotes as in amniotes, but that transcriptional quiescence of post-meiotic spermatozoa is not a pan vertebrate phenomenon. On the contrary, the data show that the identified signal transduction pathways between the soma and the sperm upregulate effector genes essential for maturational competence and male fertility.

show abstract

Sperm motility modulated by Trpv1 regulates zebrafish fertilization

Cited by 14 publications

References 41 publications

Structure and Function of Ion Channels Regulating Sperm Motility—An Overview

Structure and Function of Ion Channels Regulating Sperm Motility—An Overview

Toxicity of Modified Magnetite-Based Nanocomposites Used for Wastewater Treatment and Evaluated on Zebrafish (Danio rerio) Model

Protamine lacking piscine spermatozoa are transcriptionally active

Contact Info

Product

Resources

About