Sperm motility of Rhamdia quelen studied using computer-assisted analysis by open-source software

Sanches, Eduardo Antônio; Bombardelli, Robie Allan; Marcos, Ronan Maciel; Neumann, Giovano; Toledo, César Pereira Rebechi de; Romagosa, Elizabeth

doi:10.1111/j.1365-2109.2010.02598.x

Cited by 30 publications

(31 citation statements)

References 9 publications

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“…That fact may be observed when compared with other species, such as Rhamdia quelen, which exhibits oocytes with average diameter from 1.0mm (Pereira et al, 2006) (Bombardelli et al, 2006). Sanches et al (2010), studying the same species, observed the VAP of 58.67±9.01µm s -1 and progression of 1,202.80±163.89µm 15 seconds after activation, unlike S. parahybae, which despite exhibiting larger eggs, also exhibits higher values of VAP and PROG, and even so needs higher inseminating doses. Therefore, more studies are necessary to clarify those effects on the species.…”

Section: Discussionmentioning

confidence: 98%

“…Furthermore, the computerized analysis of sperm motility was conducted as proposed by Sanches et al (2010;2013).…”

Section: Methodsmentioning

confidence: 99%

“…The evaluation of sperm motility by the computerized method was carried out as described by Wilson-Leedy & Ingermann (2007) and adapted for neotropical catfishes by Sanches et al (2010;2013), using free software. The process of activation was conducted in Eppendorf tubes (1.5mL), using 1µL of semen and 1,000µL of water from the incubation system.…”

Section: Methodsmentioning

confidence: 99%

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Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

et al. 2016

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The Steindachneridion parahybae is an endangered catfish from Brazil and strategies applied for gametes optimization are necessary. The aim of this study was to assess inseminating doses and water volume upon the fertilization, hatching rates and percentage of normal larvae in S. parahybae. Was used a randomized design in factorial scheme (4×4) with four inseminating doses: 1.0×10 4 , 1.0×10 5 , 1.0×10 6 , 1.0×10 7 spermatozoa oocyte -1 and four volumes of water: 1, 35, 65 and 95mL of water g -1 of oocytes. The combination of doses and volumes were performed in triplicates (n=48). Each incubator (1.5L of useful volume) with 1g of oocytes was considered as an experimental unit. Significant interaction between inseminating doses and volumes of water to the values of the fertilization rates and quadratic effect of doses and volume for the values of hatching rates were observed. The doses and volumes did not influence the percentage of normal larvae (87.70±5.06%). It is recommended the use of 5.5×10 6 spermatozoa oocyte -1 and 1mL of water g -1 of oocytes during in vitro fertilization procedure. These results allowed us to develop new biotechnological strategies applied to the conservation of S. parahybae.O Steindachneridion parahybae é um bagre ameaçado de extinção no Brasil e estratégias aplicadas para a otimização de gametas são necessárias. O objetivo deste estudo foi avaliar doses inseminantes e volume de água sobre os valores das taxas de fertilização, eclosão e larvas normais em S. parahybae. Utilizando-se um delineamento experimental casualizado em esquema fatorial (4×4), com quatro doses inseminantes: 1,0×10 4 ; 1,0×10 5 ; 1,0×10 6 ; 1,0×10 7 espermatozóides ovócito -1 e quatro volumes de água: 1; 35; 65 e 95mL de água g -1 de ovócitos. As combinações de doses e volumes foram realizadas em triplicatas (n=48). Cada incubadora (1,5L de volume útil) contendo 1g de ovócitos foi considerada como uma unidade experimental. Interações significativas entre doses inseminantes e volumes de água para os valores das taxas de fertilização e efeito quadrático das doses e do volume para os valores das taxas de eclosão foram verificadas. As dosagens e os volumes aplicados não influenciaram no percentual de larvas normais (87,70±5,06). Recomenda-se a aplicação de 5,5×10 6 espermatozoides ovócito -1 e a utilização de 1mL de água.g -1 de ovócitos no procedimento de fertilização artificial in vitro. Estes resultados permitiram desenvolver novas estratégias biotecnológicas aplicadas na conservação do S. parahybae.

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Section: Discussionmentioning

confidence: 98%

“…Furthermore, the computerized analysis of sperm motility was conducted as proposed by Sanches et al (2010;2013).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

et al. 2016

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“…The requirements for vitamins may be affected by intrinsic factors to the animal (growth rate, body size, species, reproductive status), diet composition and interaction with other nutrients and are also influenced by the production system (Sanches, Bombardelli, & Marcos, 2010;Combs, 2012).…”

Section: Introductionmentioning

confidence: 99%

<b>Vitamin C supplementation on growth performance and gonadal development in Nile tilapia

et al. 2016

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ABSTRACT. The study aimed to verify the vitamin C supplementation on performance and gonadal development of Nile tilapia. 160 fingerlings distributed in 16 tanks with 65 L capacity and constant water renewal in a closed recirculation system were used. The design was completely randomized with four treatments (0, 150, 300, and 600 mg of monophosphate L-ascorbic acid kg -1 diet) and four replications. We evaluated the weight gain parameters, apparent feed conversion, apparent feed intake and carcass yield to evaluate of production performance. We analyzed the sexual maturation stage and gonadosomatic index (GSI) to evaluate reproductive performance. There was no statistical difference in the performance parameters. It was observed significant difference to the GSI. No significance was found regarding other reproduction parameters. We recommend the use of 150 mg kg Suplementação de vitamina C sobre o desempenho e desenvolvimento gonadal de tilápia-do-Nilo RESUMO. O estudo objetivou verificar a suplementação de vitamina C sobre o desempenho produtivo e desenvolvimento gonadal da tilápia-do-Nilo. Foram utilizados 160 alevinos, distribuídos em 16 aquários com capacidade de 65 L e renovação de água constante em sistema fechado de recirculação. O delineamento foi inteiramente casualizado com quatro tratamentos (0, 150, 300, e 600 mg de monofosfato de ácido L-ascórbico kg -1 de ração ) e quatro repetições. Foram avaliados os parâmetros de ganho em peso, conversão alimentar aparente, consumo aparente de ração e rendimento de carcaça para avaliação de desempenho produtivo. Foram analisados o estádio de maturação sexual e índice gonadossomático (IGS) para avaliação de desempenho reprodutivo. Não houve diferença estatística para os parâmetros de desempenho. Foi observado diferença significativa para o índice gonadossomático. Não foi observada significância em relação a outros parâmetros reprodutivos. Recomenda se a utilização de 150 mg kg -1 de Vitamina C à ração de tilápia-do-Nilo para o índice reprodutivo.Palavras-chave: ácido ascórbico, Oreochromis niloticus, reprodução.

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“…Considering the different approaches of the studies, it seems that specific times or intervals were chosen according to the particular characteristics of each type of sperm, the main objective of the study, or difficulties experienced during milt manipulation. Therefore, of the articles that used the CASA plug-in, the analyses were performed at a single time (Fauvel et al, 2012), at regular intervals for whole duration of motility Mylonas et al, 2013), or at regular intervals during part of the motility period (Sanches et al, 2010;Boryshpolets et al, 2013;Kalbassi et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Contribution of the CASA system, based on open source software, to the assessment of sperm characteristics: informetric and usage-based approaches

et al. 2017

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-By using informetric tools, we evaluated the contribution and impact of the open source computer-assisted sperm analysis (CASA) software on sperm studies, especially pertaining to fish. The article was cited 125 times between 2007 and 2014 in four publication types (articles, doctoral dissertations, master's theses, and books). Among articles, the number of citations increased at rate of three citations per year and the calculated h-index was 17. Of the studies that cited Wilson-Leedy and Ingermann (2007), fish were the main group of organisms studied (61 articles); of these, 34 articles used the open source CASA, covering 23 marine or freshwater species. Studies conducted in 12 countries were published in 21 journals. Four ways of knowledge sharing were identified: by direct partnership with the system developers; by knowledge multipliers, who worked directly with the system developers; by groups that adopted the system by their own initiative; and through expansion of cooperation networks with autodidacts. In regard to specific analytical procedures, motility, curvilinear velocity, average path velocity, and straight-line velocity were the main CASA parameters used to describe sperm movements. As expected, the usage of this tool has been increasing over the years, being a reliable and versatile alternative to more costly similar platforms. However, the potential of the CASA system has not been completely explored and it is our assessment that some results produced by CASA are poorly understood or even misunderstood.Key Words: computational analysis, fish, motility, scientometry, spermatozoa Revista Brasileira de Zootecnia

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Sperm motility of Rhamdia quelen studied using computer-assisted analysis by open-source software

Cited by 30 publications

References 9 publications

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

Inseminating dose and water volume applied to the artificial fertilization of Steindachneridion parahybae (Steindachner, 1877) (Siluriformes: Pimelodidae): Brazilian endangered fish

<b>Vitamin C supplementation on growth performance and gonadal development in Nile tilapia

Contribution of the CASA system, based on open source software, to the assessment of sperm characteristics: informetric and usage-based approaches

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