Sperm of Fruit Fly Drosophila melanogaster under Space Flight

Zhdankina, Yulia S.; Kotov, Oleg V.

doi:10.3390/ijms23147498

Cited by 7 publications

(4 citation statements)

References 46 publications

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“…This was confirmed by data on the motility of spermatozoa of the fruit fly Drosophila melanogaster after a 12-day space flight [ 100 ]. Due to a number of technical problems, it is not possible to conduct lifetime experiments at the landing site.…”

Section: Gametsmentioning

confidence: 56%

“…Such protein kinases, the action of which will lead to a decrease in the motor activity of spermatozoa, can be, for example, glycogen synthase kinase 3 (GSK3) and phosphatidylinositol 3-kinase (PI3K). It should be noted, as the main result of this experiment, that the use of 6-DMAP makes it possible to restore the reduced speed of spermatozoa in the flight group to the control level [ 100 ].…”

Section: Gametsmentioning

confidence: 99%

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Single Cell in a Gravity Field

Ogneva

2022

Life

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The exploration of deep space or other bodies of the solar system, associated with a long stay in microgravity or altered gravity, requires the development of fundamentally new methods of protecting the human body. Most of the negative changes in micro- or hypergravity occur at the cellular level; however, the mechanism of reception of the altered gravity and transduction of this signal, leading to the formation of an adaptive pattern of the cell, is still poorly understood. At the same time, most of the negative changes that occur in early embryos when the force of gravity changes almost disappear by the time the new organism is born. This review is devoted to the responses of early embryos and stem cells, as well as terminally differentiated germ cells, to changes in gravity. An attempt was made to generalize the data presented in the literature and propose a possible unified mechanism for the reception by a single cell of an increase and decrease in gravity based on various deformations of the cortical cytoskeleton.

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Section: Gametsmentioning

confidence: 56%

Section: Gametsmentioning

confidence: 99%

Single Cell in a Gravity Field

Ogneva

2022

Life

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“…The shape of cells is determined primarily by the cytoskeleton. In real and simulated micro-(2D or 3D clinorotation by random position machine) and hypergravity (by centrifugation), a change in the structure of all types of filaments involved in the formation of the cytoskeleton was shown, and experiments were performed on cells of various types and ls of differentiation [4][5][6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

The Mechanoreception in Drosophila melanogaster Oocyte under Modeling Micro- and Hypergravity

Ogneva

2023

Cells

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The hypothesis about the role of the cortical cytoskeleton as the primary mechanosensor was tested. Drosophila melanogaster oocytes were exposed to simulated microgravity (by 3D clinorotation in random directions with 4 rotations per minute—sµg group) and hypergravity at the 2 g level (by centrifugal force from one axis rotation—hg group) for 30, 90, and 210 min without and with cytochalasin B, colchicine, acrylamide, and calyculin A. Cell stiffness was measured by atomic force microscopy, protein content in the membrane and cytoplasmic fractions by Western blotting, and cellular respiration by polarography. The obtained results indicate that the stiffness of the cortical cytoskeleton of Drosophila melanogaster oocytes decreases in simulated micro- (after 90 min) and hypergravity (after 30 min), possibly due to intermediate filaments. The cell stiffness recovered after 210 min in the hg group, but intact microtubules were required for this. Already after 30 min of exposure to sµg, the cross-sectional area of oocytes decreased, which indicates deformation, and the singed protein, which organizes microfilaments into longitudinal bundles, diffused from the cortical cytoskeleton into the cytoplasm. Under hg, after 30 min, the cross-sectional area of the oocytes increased, and the proteins that organize filament networks, alpha-actinin and spectrin, diffused from the cortical cytoskeleton.

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“…However, we did not evaluate the fecundity of flies either in space flight or on Earth. At the same time, a decrease in the speed of movement of spermatozoa and a violation of the structure of their cytoskeleton in the early period of readaptation to gravity after a space flight [ 19 ] require both an analysis of the structure of the female germ cell and, in general, an assessment of the reproductive potential.…”

Section: Introductionmentioning

confidence: 99%

Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity

Golubkova

Biryukov

Kotov

2022

Cells

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The effect of space flight factors and the subsequent adaptation to the Earth’s gravity on oocytes is still poorly understood. Studies of mammalian oocytes in space present significant technical difficulties; therefore, the fruit fly Drosophila melanogaster is a convenient test subject. In this study, we analyzed the structure of the oocytes of the fruit fly Drosophila melanogaster, the maturation of which took place under space flight conditions (the “Cytomehanarium” experiment on the Russian Segment of the ISS during the ISS-67 expedition). The collection of the oocytes began immediately after landing and continued for 12 h. The flies were then transferred onto fresh agar plates and oocyte collection continued for the subsequent 12 h. The stiffness of oocytes was determined by atomic force microscopy and the content of the cytoskeletal proteins by Western blotting. The results demonstrated a significant decrease in the stiffness of oocytes in the flight group compared to the control (26.5 ± 1.1 pN/nm vs. 31.0 ± 1.8 pN/nm) against the background of a decrease in the content of some cytoskeletal proteins involved in the formation of microtubules and microfilaments. This pattern of oocyte structure leads to the disruption of cytokinesis during the cleavage of early embryos.

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Sperm of Fruit Fly Drosophila melanogaster under Space Flight

Cited by 7 publications

References 46 publications

Single Cell in a Gravity Field

Single Cell in a Gravity Field

The Mechanoreception in Drosophila melanogaster Oocyte under Modeling Micro- and Hypergravity

Drosophila melanogaster Oocytes after Space Flight: The Early Period of Adaptation to the Force of Gravity

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