Sperm penetration into immature mouse oocytes and nuclear changes during maturation: an EM study

Szöllősi, Dániel; Szöllösi, Maria S.; Czołowska, Renata; Tarkowski, Andrzej

doi:10.1016/0248-4900(90)90328-z

Cited by 28 publications

(36 citation statements)

References 46 publications

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“…Szollosi et al (1990) have reported that although the sperm centriole is introduced into mouse oocytes at fertilization, it is not capable of behaving-as a centrosome and organizing microtubules, as was shown in the case of the human. Further differences in the expression of the embryonic genome, which occurs at the two-cell stage in the mouse (Dvorak et al, 1985) and between the four to eight-cell stage in the human (Sathananthan, 1992;Tesarik et al, 1988) may well necessitate the identification of an alternative mammalian model for both examining basic mechanisms and developing innovative techniques leading to successful ART.…”

Section: Resultsmentioning

confidence: 89%

Ultrastructural changes during meiotic maturation in mammalian oocytes: Unique aspects of the human oocyte

Sathananthan

1994

Microscopy Res & Technique

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This paper reviews the process of peri-ovulatory oocyte maturation and the ultrastructural organization of the human egg and compares it with that of the mouse. The main thrust of the paper is on the human, since there are several reviews on the mouse. Both preovulatory and postovulatory events at fertilization, as well as some of the aberrant features of maturation are covered. Some changes induced by oocyte culture and cooling in the human are also included. The report attempts to focus on unique features of the human oocyte and shows a variety of ultrastructural differences between human and murine oocytes, which may well reflect differences in their physiology and biochemistry. Based on these differences and further observations on the process of fertilization of both species, particularly with respect to the inheritance of paternal centrioles, it is concluded that the mouse may not be a suitable model for the development and refinement of current procedures in human assisted reproductive technology.

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Section: Resultsmentioning

confidence: 89%

Ultrastructural changes during meiotic maturation in mammalian oocytes: Unique aspects of the human oocyte

Sathananthan

1994

Microscopy Res & Technique

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“…In rodent oocytes and early embryos, the presence of conspicuous intranuclear particles or granules has previously been reported (Palombi and Viron, 1977;Fakan and Odartchenko, 1980;Szollosi et al, 1990). In early embryos, it was concluded that the appearance of some of these particles was related to the progress of the nuclear changes during fertilization (Szollosi et al, 1990;Takeuchi and Takeuchi, 1989).…”

Section: Discussionmentioning

confidence: 87%

“…In early embryos, it was concluded that the appearance of some of these particles was related to the progress of the nuclear changes during fertilization (Szollosi et al, 1990;Takeuchi and Takeuchi, 1989). Certain categories of such granules were found to be similar to interchromatin or perichromatin granules, both of which are extranucleolar particles rich in ribonucleoproteins (Fakan, 1986), but a special category of larger granules (300-500 A in diameter) has also been identified in groups in the nucleoplasm of early mouse embryos, sometimes in the immediate vicinity of nucleoli (Fakan and Odartchenko, 1980).…”

Section: Discussionmentioning

confidence: 97%

DNA Synthesis and pronucleus development in pig zygotes obtained in vivo: An autoradiographic and ultrastructural study

Laurinčík

Hyttel²,

Kopečný

1995

Molecular Reproduction Devel

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Porcine zygotes flushed from oviducts 48, 52, 56, 60, or 64 hr after hCG were incubated 30 min in 3H-thymidine, transferred to nonradioactive medium for 2 hr, and incubated for 30 min with 14C-thymidine. After this procedure, ova were prepared (i.e., at 51, 55, 59, 63, or 67 hr after hCG) for autoradiography and ultrastructural observations, respectively. The first autoradiographic labelling, i.e., DNA synthesis, was observed at 56-56.5 hr after hCG, while the latest labelling was seen at 60-60.5 hr. At 51 hr after hCG, formation of the pronuclear envelope was observed, while no nucleolus precursor bodies or prestages to these structures were found. At 55 hr a few clusters of small electron-dense granules were observed, together with condensed chromatin in the pronuclei. At 59 hr the apposed regions of both pronuclei contained nucleolus precursor bodies and condensed chromatin, in close contact with both clusters of small granules and clusters of an additional category of large granules and the nuclear envelope. Additionally, large accumulations of the small granules were found in the vicinity of similarly sized accumulations of the large granules without chromatin association. At 63 hr the spherical accumulations of large granules on some occasions presented a central vacuole, and condensed chromatin and clusters of small granules were attached to its periphery. Within the vacuole, electron-dense material was found.(ABSTRACT TRUNCATED AT 250 WORDS)

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“…However, the decondensastion of sperm chromatin does not necessarily indicate that this chromatin can undergo transformation into male pronucleus. In collaboration with Dan and Maryna Szollosi, we have demonstrated that the breakdown of the spermatozoon's nuclear envelope is a prerequisite for the transformation of the sperm nucleus into functional pronucleus (Borsuk and Tarkowski, 1989;Maleszewski 1990, 1992, Szollosi et al, 1990, Borsuk, 1991. However, Szollosi et al (1994) showed that the decondensation of sperm chromatin is not fully preventable by the lack of sperm nuclear envelope breakdown.…”

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confidence: 99%

Mammalian and avian embryology at Warsaw University (Poland) from XIX century to the present

Tarkowski

Maleszewski²,

Rogulska³

et al. 2008

Int. J. Dev. Biol.

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KEY WORDS: mouse, regulative development, chimaerism, cell lineage, fertilization The beginnings: XIX century -1939Poland lost independence in the XVIII century after she was partitioned between Russia, Prussia and Austria from 1772 to 1795. In the times that followed, many Polish scientists studied and often lived abroad, and many foreigners came to work in Polish academic institutions. In 1816 Alexander I, the Emperor of Russia and the usurper King of Poland, granted permission for the establishment of Warsaw University. The University followed the tradition of three earlier institutions of higher education: Collegium Nobilium, opened in 1766, the School of Law (founded in 1808) and the School of Medicine (founded in 1809). The University still bears the emblem described as "an ancient Polish eagle with a crown, with spread wings, a palm frond in its left claws and laurel branch in its right, the symbols of patient diligence and honour, surrounded by five stars representing its five academic faculties". These faculties were theology, law, medicine, philosophy, and the liberal arts. After the fall of the November 1830 uprising, in which a majority of students had participated, the University was closed in 1831. In 1857 there was a brief period of Int. J. Dev. Biol. 52: 121-134 (2008) +48-22-55-41-210. e-mail: akt@biol.uw.edu.pl # Note: This article is not a review of the principle interests of this Department, and therefore, we quote other authors very selectively, for instance, when they carried out concurrently similar studies, or when their studies are essential for the understanding of the rationale and/or implications of our experiments. liberalization that resulted in the opening of the Main School (Szkola Glowna) with the departments of medical-surgical, law and administration, philological-historical, and mathematics-physics. The Main School was closed in 1869, on the wave of increasing oppression and russification after the fall of the second, the January 1863 uprising. However, in the short period of the existence of the Main School as many as three thousand students graduated and the School had great impact on the development of Polish science and culture. In 1870, the Russian Imperial University was opened in Warsaw. The majority of faculty teachers were Russians and the official (teaching) language was Russian. Steadily the University expanded: new buildings were constructed; academic potential increased and precious zoological and botanical collections were created.The Department of Comparative Anatomy was founded in 1870. It was headed by Russian scientists: Mitrofan Ganin (1870-84), Mitrofan Uljanin (1884-1888) and Pawel Mitrofanow (Mitrophanow) . Mitrofanow ( Fig. 1) was an outstanding avian embryologist working mainly on early embryos; in papers published in 1895 and 1899, he presented evidence that 122 A. Tarkowski et al. which in 1918 -when Poland regained independencebecame one of the first legally functioning Polish national institutions. Soon, many distinguished specialists and...

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Sperm penetration into immature mouse oocytes and nuclear changes during maturation: an EM study

Cited by 28 publications

References 46 publications

Ultrastructural changes during meiotic maturation in mammalian oocytes: Unique aspects of the human oocyte

Ultrastructural changes during meiotic maturation in mammalian oocytes: Unique aspects of the human oocyte

DNA Synthesis and pronucleus development in pig zygotes obtained in vivo: An autoradiographic and ultrastructural study

Mammalian and avian embryology at Warsaw University (Poland) from XIX century to the present

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