Sperm protein (sp50) binds to acromosome and plasma membranes in a Ca
            <sup>2+</sup>
            ‐dependent manner: Possible role in acrosome reaction

Hernández, Enrique Othón; Roa-Espitia, Ana Lilia; Trejo, Juana Cruz; Mújica, Adela

doi:10.1002/(sici)1098-2795(199603)43:3<366::aid-mrd11>3.0.co;2-#

“…Membrane proteins were analyzed by SDS-PAGE [Laemmli, 1970] and transferred to nitrocellulose membranes [Towbin et al, 1979] for immunostaining with anti-actin, anti-spectrin or anti-Na ϩ ,K ϩ -ATPase antibodies using a previous protocol [Moreno-Fierros et al, 1992]. Actin was detected with the monoclonal anti-actin antibody diluted 1:10, spectrin with anti-human spectrin antibody diluted 1:200 and the Na ϩ ,K ϩ -ATPase pump with anti-Na ϩ ,K ϩ -ATPase antibody diluted 1:10 with blocking solution [Hernández et al, 1996].…”

Section: Western Blottingmentioning

confidence: 99%

Involvement of an F-actin skeleton on the acrosome reaction in guinea pig spermatozoa

Hernández‐González¹,

Lecona-Valera

²

,

Escobar-Herrera

³

et al. 2000

Cell Motil. Cytoskeleton

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“…In mice, varying stages of germ cells exist in pubertal seminiferous tubule [2]. The expression of high Mr CBPs such as molecular chaperone involved in spermatogenesis [4], protein phosphatase [11] calmodulin [9], Ca 2+ sequestering molecule [10], antigen involved in membrane fusion of spermatozoa [13], and acrosomal component [12] have been reported in testis. Therefore, this result coincided with the structural and functional differentiation of cellular components of seminiferous epithelia at puberty.…”

Section: Discussionmentioning

confidence: 73%

Postnatal Changes in the Calcium Binding Proteins of Mouse Testis

Gye

¹

,

Kim²,

Ahn³

et al. 2001

Archives of Andrology

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To define the changes in calcium-binding proteins (CBPs) of mouse testis during postnatal development, testicular protein extracts were analyzed by mobility shift after Ca2+ binding (MSACB) in the 2-dimensional SDS-PAGE. In the adult testis extracts, 29 kinds of protein spots showed mobility shift in the gel containing Ca2+. Among them, 2 spots showed nonspecific mobility shift and 4 spots showed Zn2+ shift. Drastic changes in expression of high-Mr CBPs (Mr > 25 kDa) were observed in testis 3 weeks after birth, suggesting that their expressions were developmentally changed. There was no obvious difference in the qualitative patterns of CBP between 4-week-old testis and adult testis. There was a large mass of CBPs (Mr = 20-25 kDa) expressed constitutively in testis throughout the postnatal development. Degree of MSACB (Mr on the diagonal line/Mr of shifted point in Ca2+ gel) of each spot was in the range of 1.1-1.7 in 12% acrylamide gel. These results suggest that changes in CBPs of testis are closely related with progression of spermatogenesis in mouse testis.

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“…In mice, varying stages of germ cells exist in pubertal seminiferous tubule [2]. The expression of high Mr CBPs such as molecular chaperone involved in spermatogenesis [4], protein phosphatase [11] calmodulin [9], Ca 2+ sequestering molecule [10], antigen involved in membrane fusion of spermatozoa [13], and acrosomal component [12] have been reported in testis. Therefore, this result coincided with the structural and functional differentiation of cellular components of seminiferous epithelia at puberty.…”

Section: Discussionmentioning

confidence: 99%

Postnatal Changes in the Calcium Binding Proteins of Mouse Testis

Gye

¹

2001

Archives of Andrology

2

0

View full text Add to dashboard Cite

To define the changes in calcium-binding proteins (CBPs) of mouse testis during postnatal development, testicular protein extracts were analyzed by mobility shift after Ca 2+ binding (MSACB) in the 2-dimensional SDS-PAGE. In the adult testis extracts, 29 kinds of protein spots showed mobility shift in the gel containing Ca 2+. Among them, 2 spots showed nonspecific mobility shift and 4 spots showed Zn 2+ shift. Drastic changes in expression of high-M r CBPs (M r > 25 kDa) were observed in testis 3 weeks after birth, suggesting that their expressions were developmentally changed. There was no obvious difference in the qualitative patterns of CBP between 4-week-old testis and adult testis. There was a large mass of CBPs (M r = 20-25 kDa) expressed constitutively in testis throughout the postnatal development. Degree of MSACB (M r on the diagonal line/M r of shifted point in Ca 2+ gel) of each spot was in the range of 1.1-1.7 in 12% acrylamide gel. These results suggest that changes in CBPs of testis are closely related with progression of spermatogenesis in mouse testis. Keywords calcium binding protein, mouse, testis Calcium plays an important role in many biological processes. Many of these events are probably mediated by interactions with a variety of calcium binding proteins (CBPs). Several CBPs in male germ cells, including sperm, were identified. These proteins include molecular chaperone involved in spermatogenesis [4], protein phosphatase [11], calmodulin [9], Ca 2+ sequestering molecule [10], antigen involved in membrane fusion of spermatozoa [13], and acrosomal component [12]. During spermatogenesis, the expression of CBPs is important for the functional differentiation of germ cells as well as general cellular process [14]. During The present study was supported by the Ministry of Public Health and Welfare of Korea (HMP-98-M-1-0017) .

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Sperm protein (sp50) binds to acromosome and plasma membranes in a Ca ²⁺ ‐dependent manner: Possible role in acrosome reaction

Cited by 8 publications

References 46 publications

Involvement of an F-actin skeleton on the acrosome reaction in guinea pig spermatozoa

Involvement of an F-actin skeleton on the acrosome reaction in guinea pig spermatozoa

Postnatal Changes in the Calcium Binding Proteins of Mouse Testis

Postnatal Changes in the Calcium Binding Proteins of Mouse Testis

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Sperm protein (sp50) binds to acromosome and plasma membranes in a Ca 2+ ‐dependent manner: Possible role in acrosome reaction

Cited by 8 publications

References 46 publications

Involvement of an F-actin skeleton on the acrosome reaction in guinea pig spermatozoa

Involvement of an F-actin skeleton on the acrosome reaction in guinea pig spermatozoa

Postnatal Changes in the Calcium Binding Proteins of Mouse Testis

Postnatal Changes in the Calcium Binding Proteins of Mouse Testis

Contact Info

Product

Resources

About

Sperm protein (sp50) binds to acromosome and plasma membranes in a Ca ²⁺ ‐dependent manner: Possible role in acrosome reaction