Spermatic and oxidative profile of domestic cat (<i>Felis catus</i>) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Angrimani, Daniel de Souza Ramos; Nagai, Ken Kawaoka; Rui, Bruno Rogério; Bicudo, L. C.; Losano, João Diego de Agostini; Brito, Maíra Morales; Francischini, Maria Claudia Pereda; Nichi, Marcílio

doi:10.1111/rda.13086

Cited by 8 publications

(5 citation statements)

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“…All samples used in this study presented adequate sperm quality in the evaluations of total motility, progressive motility and viability (Angrimani et al, ; Emerenciano et al, ; Jelinkova, Vitasek, Novotny, & Bartoskova, ). The variation in the percentage of normal sperm (55 to 78.5% in this study) is common among felines, and the same animal may present a normospermic and teratospermic sample as a result of environmental stress, diet and/or age (Pukazhenthi et al, ).…”

Section: Discussionmentioning

confidence: 85%

Morphological and morphometric characterization of domestic cat epididymal sperm

Barbosa

Silva

Tabosa

et al. 2019

Reprod Domestic Animals

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Sperm morphometry is the tool that confers objectivity to the morphological evaluation by accurately measuring the dimensions of the gamete and its structures. Thus, the aim of the study was to perform a morphometric characterization of the domestic cat sperm. Therefore, sperm samples were collected from twenty pairs of epididymis in a TRIS extender at 37ºC. An aliquot of the sample was used to make a smear with Rose Bengal solution, and afterwards, the morphology and morphometry were analysed. In the morphology, were quantified the percentage of normal sperm cells, morphological changes of head, midpiece and tail. In morphometry, each normal sperm cell was measured for length, width, area and perimeter of head and midpiece, tail length and total length. The parameters ellipticity, elongation, regularity and rugosity were also determined. The percentage of normal sperm was 67.21%. Of the abnormalities, the curled/folded tail, followed by the curved midpiece, abnormal shaped head and detached head were the most quantified. The sperm head presented 5.56 ± 0.01 μm and 3.10 ± 0.01 μm of length and width, respectively. The head area was 16.94 ± 0.05 μm2, while the perimeter was 16.16 ± 0.03 μm. In the derived parameters, the values were as follows: ellipticity of 1.81 ± 0.00; elongation of 21.39 ± 0.12; regularity of 0.81 ± 0.00; and rugosity of 0.14 ± 0.00. The midpiece presented length and width of 7.96 ± 0.01 μm and 0.76 ± 0.01 μm, respectively. The mean length of the sperm tail was 45.12 ± 0.06 μm, and the total cell size was 58.67 ± 0.06 μm. Thus, it was concluded that the cat sperm is an elongated cell, with high rugosity and regularity. The spermatic tail represents more than ¾ of the total length of the cell and the midpiece exceeds the length of the head.

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Section: Discussionmentioning

confidence: 85%

Morphological and morphometric characterization of domestic cat epididymal sperm

Barbosa

Silva

Tabosa

et al. 2019

Reprod Domestic Animals

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“…In comparison, post-thaw epididymal cat spermatozoa did not experience an increase in lipid peroxidation after 6 h of incubation at 37°C ( 31 ), while frozen-thawed human spermatozoa showed increased lipid peroxidation after being incubated for 15 to 60 mins at the same temperature ( 32 ). Additionally, cold storage of equine spermatozoa for 48 h led to a significant increase in lipid peroxidation ( 33 ), whereas cat epididymal sperm stored at 5°C maintained high quality for up to 48 h of cooling and were not exposed to oxidative stress until after 72 h of cooling ( 34 ). It can be hypothesized that high levels of endogenous antioxidant activity may be present in cat spermatozoa and/or epididymal fluid that may neutralize excessive ROS concentrations during semen processing and cryopreservation.…”

Section: Discussionmentioning

confidence: 99%

Mito-Tempo improves acrosome integrity of frozen-thawed epididymal spermatozoa in tomcats

Ali Hassan,

Banchi,

Domain

et al. 2023

Front. Vet. Sci.

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IntroductionIn tomcats, epididymal spermatozoa provide an additional source of male gametes available for cryopreservation. While this procedure is feasible, the survival rate and motility of epididymal cat spermatozoa are both low after thawing. Cryopreservation is known to induce oxidative stress in spermatozoa, with mitochondria and the plasma membrane being the two major generation sites, and an imbalanced presence of free radicals is a possible cause for this low survival rate. Different antioxidants have been tested before for their effect on cryopreserved cat spermatozoa quality, with varying results. Here, we used Mito-Tempo, which is a synthetic mitochondria-targeted antioxidant and a specific scavenger of the mitochondrial superoxide system. By supplementing Mito-Tempo with the freezing extender, we aimed to improve the sperm quality of frozen-thawed cat epididymal spermatozoa.MethodsEpididymal spermatozoa obtained from twelve tomcats were assessed for motility and concentration. Prior to freezing, samples were diluted in TRIS buffered extender with egg yolk and glycerol and divided into five aliquots supplemented with 0 (control), 0.5, 5, 50, and 1005M of Mito-Tempo. After thawing, sperm motility, concentration, morphology, plasma membrane integrity, acrosome integrity, and mitochondrial membrane potential were evaluated. A Friedman rank sum test with a Bonferroni post-hoc test was used to determine statistical in-between group differences in post-thaw semen parameters.Results and discussionThe results indicated a slight improvement in acrosome integrity across all groups that were supplemented with Mito-Tempo, with the group that received 55M of Mito-Tempo showing the greatest improvement [(median of 67.99%, IQR of 5.55) compared to the control group (median of 65.33%, IQR of 7.75; P = 0.05)]. For all other sperm parameters, no significant differences (P > 0.05) were detected between different Mito-Tempo concentrations. These findings highlight the protective effect of Mito-Tempo on acrosome integrity and suggest that 55M is the most effective concentration for maintaining acrosome integrity. Since Mito-Tempo has shown a positive effect on multiple sperm parameters in other species, such as men, boars, roosters, rams, and bulls, we need to conclude that species-specificity may play a role here.

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“…In this study, the decline in post-thaw viability and functionality results from the physical-osmotic changes undergone by the sperm cells and the formation/dissolution of ice crystals during freezing-thawing, which can cause structural changes in the membrane, generate ruptures, or loss of osmoregulation capacity (Figueroa et al, 2016). Changes in the plasma membrane also result from the lipid peroxidation, due to the overproduction of ROS in the cryopreservation process, and the interaction of these with the membrane biomolecules, especially fatty acids (Angrimani et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa

Gomes

Fernandes

Izzo

et al. 2021

RSD

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This study evaluated the effect of different concentrations of Brazilian green propolis on the cryopreservation of epididymal sperm from domestic cats. Spermatozoa were collected from cauda epididymis by slicing technique in a TRIS extender, preheated to 37ºC. Then, the sperm were evaluated and divided randomly between the control group (without adding propolis in the extender) and the treatment groups (adding different concentrations of propolis extract in the extender): P1 (0.1 mg/mL), P2 (0.3 mg/mL) e P3 (0.6 mg/mL). Subsequently, sperm were cryopreserved. The evaluated parameters were sperm kinetics by the Computer Assisted Semen Analysis, vigor, viability, membrane functionality, chromatin condensation and morphology. The parameters were measured before and after cryopreservation. Propolis didn’t show toxicity to the sperm in any concentrations, with no changes observed in the motility pattern. There was no significant influence of propolis on motility, vigor, percentage of viable spermatozoa, chromatin quality and other kinetic parameters in the cryopreserved samples. In sperm morphology, it was demonstrated a reduction in the percentage of normal cells in P1 and P2 groups in relation to the fresh sample, control and P3 group. In addition, an interesting effect from the propolis groups was observed on possible growth inhibition of microorganisms in the contaminated samples. Propolis didn’t provide superiority to the sperm parameters evaluated after thawing, except for the values of plasma membrane functionality, which were better. The propolis is an interesting component to be incorporated in the cryoprotectant medium due to its non-toxicity and the potential inhibition of microbial growth.

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Spermatic and oxidative profile of domestic cat (Felis catus) epididymal sperm subjected to different cooling times (24, 48 and 72 hours)

Cited by 8 publications

References 59 publications

Morphological and morphometric characterization of domestic cat epididymal sperm

Morphological and morphometric characterization of domestic cat epididymal sperm

Mito-Tempo improves acrosome integrity of frozen-thawed epididymal spermatozoa in tomcats

Brazilian green propolis doesn't have a beneficial effect on the cryopreservation of domestic cat epididymal spermatozoa

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